LOCUS       sequence-174186-        2865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNAi expression vector based on H1 promoter and pUC18 vector 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Mathupala SP, Parajuli P, Sloan AE
  TITLE     Silencing of monocarboxylate transporters via small interfering 
            ribonucleic acid inhibits glycolysis and induces cell death in 
            malignant glioma: an in vitro study.
  JOURNAL   Neurosurgery. 2004 Dec;55(6):1410-9; discussion 1419.
  PUBMED    15574223
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neurosurgery."; date: "2004-12"; volume: "55(6)"; pages: "1410-9; 
            discussion 1419"
COMMENT     Sequence Label: pUC18-H1 RNAi
FEATURES             Location/Qualifiers
     source          1..2865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      9..597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     498..517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     751..768
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    885..906
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(921..926,927..944,945..951)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    959..975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     964..986
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     983..999
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     983..999
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1015..1229
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     1150..1169
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(1248..1265)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1248..1264)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1257..1279)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1473..1492)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1592..1614
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1652..1670)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1738..1842
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1843..1911,1912..2703)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2061..2080)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
       61 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      121 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
      181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      661 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      721 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      781 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      841 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      901 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      961 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga attcgaacgc
     1021 tgacgtcatc aacccgctcc aaggaatcgc gggcccagtg tcactaggcg ggaacaccca
     1081 gcgcgcgtgc gccctggcag gaagatggct gtgagggaca ggggagtggc gccctgcaat
     1141 atttgcatgt cgctatgtgt tctgggaaat caccataaac gtgaaatgtc tttggatttg
     1201 ggaatcgtat aagaactgta tgagaccaca gatctagaaa gcttggcact ggccgtcgtt
     1261 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     1321 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     1381 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     1441 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     1501 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     1561 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     1621 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     1681 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     1741 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1801 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1861 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1921 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1981 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2041 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2101 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2161 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2221 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2281 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2341 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2401 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2461 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2521 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2581 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2641 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2701 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2761 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2821 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaa
//
