LOCUS       sequence-174190-       12553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives Bxb1 expression in the D. melanogaster germ line..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12553)
  AUTHORS   Voutev R, Mann RS
  TITLE     Bxb1 phage recombinase assists genome engineering in Drosophila 
            melanogaster.
  JOURNAL   Biotechniques. 2017 Jan 1;62(1):37-38. doi: 10.2144/000114494.
  PUBMED    28118814
REFERENCE   2  (bases 1 to 12553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2017-01-1- 1"; volume: "62"; issue: "1"; 
            pages: "37-38"
COMMENT     Sequence Label: pRVV210
FEATURES             Location/Qualifiers
     source          1..12553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2094..2105)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5830..5846
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5831..5847
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7506..9008
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     protein_bind    10077..10146
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(10365..10383)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10404..10420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10404..10420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10417..10439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10428..10444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10452..10458,10459..10476,10477..10482))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10497..10518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10635..10652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10806..11394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10886..10905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11565..12356,12357..12425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12188..12207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12426..12530)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccccccct cgacctgcag gtcaacggat cctaccctac caccaacaaa gtctttttgt
      721 ttgccccgtc tgaagtctgg ctccgatcaa aagcaaaaat ttcaattaca gtcgaatctc
      781 aagcgaccag gcgatctcaa attgagcgat taagttgaac tttccctgca cccaaacaag
      841 atcttgtgca tagctttatc aacctacttt cttgcctggt agtagtactc cagaaaatgt
      901 acagttgttg atatatttag aatttatgca tacttacatt ttttccgctt tttccgctca
      961 agaaaattgc gtaaactctt aaccttcaaa aaaaagttga tttattgtta ttttttgctt
     1021 aacataacta gataaagtat tgatttgcca cttgctcata cgtcatgtgg tttttttaac
     1081 cgctgttgcc ctatgttgtt gtctttaaat attctttaca tcaatcgagt gtgtgagaat
     1141 atacccaagt accgtgtgta ggattatgtt aaccttgatg ctgatgatgc caccacccag
     1201 attggcgggc attcacataa gtttttaacc caccatcctg gccacccggt tgggcgggtt
     1261 gatgggtggg aaatagatgg ggaccacttg tctcgtaatt tatgccgttg tgctggttga
     1321 aaatataggc ctttggcacc tccactcctg caggacccac agaatttgta gagacactaa
     1381 tactggagac tacattgcct gaattggcgg gcaaataagt gcgacttgga ggaggcggcg
     1441 aggaagccca gctggtctga ggtttctgtg gcaagacagg acgatattgt ttcgtatata
     1501 acggtggacc cattggcaaa acggcttgtt ttggtattga aacggtattt ggcggcccat
     1561 aggcgttatt cctcaaatca cacacagtat tctcaattaa agtggccaag ggagccgtgt
     1621 aaattcggaa attagtatct gaataatcca agtcagacag caagaaaacg ggcatcctat
     1681 cggatagaac ccaaacgttt ttgttctcat caattttcac atcggccgga aaaactaagc
     1741 caacgtcatc gcgatccaca atgccatgaa attgcggtga gtacggcatt gatgagtgcc
     1801 agcaacccac tgcattttga tctattaaat tgaacagctc aattccatca tcgctcatca
     1861 cacgtgaagt ggtatgggag tttggacccc gttcatctaa ggcaacaaag tcatgatagc
     1921 tatcttccgt cctggtttca tccctcaaaa tcctcgtgga tacggcaaat tgtcgatgac
     1981 ttgctaacgg actaaagtac agggtacgat aaccatccga tcgaatgggc gaaagggaca
     2041 taccaaatat accctcctcg ccccattgga agttaatacc agcgacattg aaatcgcccc
     2101 tcaatggatc ggggaaaaaa tacgaatgtg ccgagaatct ccaggacttg ttcagttccc
     2161 aggagtaagc aatcaagccg tatcccaatt catcggcaaa ataggcatat gcatcatcgc
     2221 aatttttgcc tatatccacg gcaatgttag ctatgaaagt atttggattt gtgtccacgc
     2281 caggtagctc gtatctccga attcgcgtat ccgtggtcaa gtcaaagaca tttaccgcat
     2341 aggagcacgg attagtggtg gtattgccga tgcccacggt tccagtgtcc aaaacccaca
     2401 gccgaccaca ctcatccact ttaatgcggt aggcagtggt aatactgttg gcgcaatctc
     2461 cagctgtatt tgagcgccaa tctggatacg gaattagctc cggtgaaccc gtcaaactgc
     2521 ggtccatgtt tatataggtc agagtggccg gaatcccatc acgccagcgg ggaacagtga
     2581 cgaataaccg attgccaaag tgttcgactc caacaggtag agcattttgc ggaatataat
     2641 ctccactagc cagagcttgg tcctttagtc gggtattcgg gaaagcaaag tccagctggc
     2701 tccaactata tcgctcctga agtttgtaag cagcccaaga cggcgtcacc aaggtgatca
     2761 gggtcacaag gatccaccct ttgtcctgga acattgcact tagctctaag ctgacaatca
     2821 ctcttaacac ttaagccaaa gtgtgttggc aataaaaaaa aatatatttg tttatatatg
     2881 ttcagctata gggggttctt ctctagcctt cggctgtgtg atattttttt aatcctcttc
     2941 tgtggaccgt ggcgcggtaa cgactggtgg ccataatatg tcggccgcgt tttatatgaa
     3001 ggtttttttc tccgaagacg aagacaggcc aatgaaaatg aaaacgaagg cgcgcgccaa
     3061 cttcggtggt tttagtgatt cgggtggttc agtgttcggg taatcaggtg gcttatgctg
     3121 ttcccataga tcggccaact ttgcgttttg tcttccatga ttgattttca cgcatgttta
     3181 ataaatacac tcgaatcccc caatttaatg agtcagacat cacctttcgc tgggtttggt
     3241 atgatttttg gccttcatcg acatttaaaa ctgcgctgat ttgtgccgaa atacctttcg
     3301 gtaccctaaa agattaagac ctattttaaa gttgtgacta ctatttattt aattttaggg
     3361 actttatttg acgatctaat ttcaaacaag taaattgtaa ggccaggagg ctcgtgcata
     3421 gaatgcattg ctctccaaat ttctgaaaat ctgaaagctt aatacatgac agttgtgttc
     3481 tgagaacagt gttcatcttt atcggcgact gcaaaaaatg ttctaatatg attttttagt
     3541 tcaattcatt aaaaaaatca aatcaatatt atatgaaata catgcagaca aaaatccaga
     3601 aatatcaaat aaatcgttat tataacgtgt actatgtaga gtatttaaag caggtgtttc
     3661 cttgtatact attaaattgg aactcgtgct cgtttcaatt gcttaggatt ggatttcgat
     3721 tgggcgtcac cgatgattca caattcactg agggtcaaat atttggtttc cgctagttat
     3781 tggcaggtga ttttgagcat acatatacat aaatataatt gagtgttaca taaaatttca
     3841 aaagggacga aagcgctttt aggtaattcc actagctgtt atttaaaata ttgtggtccc
     3901 tgaaaatcaa ctggatgttg ggggtgccct aagtgggtaa tgctctaatg accatcgccg
     3961 caagtaaatg ttaaaaattc gaaccgaaag cgatcgcata tatctcttgg cccaaatgca
     4021 cctgcttgct aaagaaaaaa ttgcaaaact taagtaaagt aatacaacca gccacacgca
     4081 taataaacat gcaaatgggt aaaaatacgt tttacgacct ccgcagataa agtggtttgc
     4141 attaatcctc aataatctac actttaaata aggaaaacct ctcaaaatat taaaatacaa
     4201 attaaagaat gccgtttaaa tttaatctta tttcttcgga tgtctaaaaa caatttcaaa
     4261 gctaatgtaa ccattactgc tattgaattt gtgtagtttt tggactctat atcatttatg
     4321 caatgctatt ttttcttaga agtggttcaa cttgatccct tgcccgcatt ctaaatcatt
     4381 gcacccacag aaaaataaac aaacacggtg tggcaatcga aaaatatgtt gcacaaaatt
     4441 accggcaatg ctgctcgaat ttattgcgat cggcaggctt attaaaagac acacttgaaa
     4501 taaccagtac agttttatta attaattgtg tgtgttgtgt aatgaaatgg tgtatttact
     4561 aaaaacgcaa ctaatgatgc gatcgctgca gccgttgaac ctataatttt tttcgaattg
     4621 acttgttttc aagtttttgc ttaattgtgt ggggggatgc tgcgtttgtc tgtttggtta
     4681 ctgtttttct ttttcgcttt tcagagcttg cggcaatgat ttatacgcac ttttcccaat
     4741 aattgaaaca agttttctgc cccagcacaa aacaaaacaa catcaagcgg agcgcgctaa
     4801 gtggtcaaaa aaagcttcca caaatcagat ctctttggca taattgacta acgcttatgc
     4861 aaccgaccgg caacattcga cattatttac acacaggatc acttcaactt gggcggaaag
     4921 caaagccgaa taccgcatga cattgcccga cccgtgtttc atgttttatt ttccttattc
     4981 cccacttatg gcagcctgga cattatctcc ctccctcccc tcccccctcc tacactaatt
     5041 tggaaatcca tcgtgactgc aaaacgaaaa acccgttgct caacttctct aacataagcg
     5101 gtacacagca ttttccgttt tgtgttatat atttttgtgt tttttttttt caagttcaat
     5161 gtccatggcc ctgaccgccg ggtcccctcc tttcgagctg ggattatttg gcatggccaa
     5221 attcggaagg ccgcgatcac ttcccgctta cacacaattg cacatgtccc catgacatgc
     5281 actgcgctca aactattagt accccttagt aacttaagcc aaattaaaaa taagttcatt
     5341 aatataaata aaatagtcaa tttccactgt cccgattgaa tagagaaact ttatttgtaa
     5401 attttaaatg tcgccaagga actcgctaga tatactaaac caaaaccgat tgtagctccc
     5461 gaaatcgcaa ccataagtgg aataattaga tgccaacaaa ggctttgttt gcttactgaa
     5521 taaaggttgt cgtaatttga tttctgtgta atcttgcacg aaacggaatc attacctcag
     5581 acataatttg cctgcaattg cggtggtgca cttgtctcgt gcaagcgaaa aaatcagtgc
     5641 tcgaaaaagc ttagtggata tactcgtaca tatactatga atcgtttaga ggggtaggta
     5701 aatcagcggg ctgcgttcga aatttatgag tgactctgcg acgtatttat cttaagccat
     5761 cgtcagttgc atattttatg gctattttaa accactcaat gtggtaatcg ggcgataatc
     5821 atttaatagt cgaggtcgac ggtatcgata agcttgatat ctataacaag aaaatatata
     5881 tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa
     5941 atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt
     6001 tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga
     6061 ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt
     6121 tcaagaatgc atgccctgca ggaagcttcc taggaggcct agatctgcgg ccgcgaaaca
     6181 cttcctcctc cacgctcatt ttggcccact taatttgatc agaggttagg ttaactggcg
     6241 tcggctgtcg gctttaatcg gtgctgcggg tctgcgacag ttgtttatgc ggaagccggg
     6301 ctatttcttt ttttccctcc aataaatgtt gtttactatt tttttcgttt ctgtattcgc
     6361 agaacaagtt gacagaacac cctaacgcgt tttagtgtta ccgggcggtg gcttgtcaag
     6421 aaataccttc ccatggctaa tacaattccc aactggaagt agtttgggtt tcagaagtca
     6481 acagtgttac cgtattttaa tgtacatctg gcttttaagc gagtacacac acatagcatt
     6541 gcgatgtttt ttaaacttgc actgcttgca agcaggggtc cactgtattt caatgaaaga
     6601 acagcgtgtt gggcgttttt gaattatact aattaccttc aataacaatt ccctatatat
     6661 cacttagttt taataaataa atcgtttgag tgtggtctag agatgattta ttatgatgaa
     6721 gtgcggttgt tggggacaag cgggatacgt ctgcgggtca aatcggcggt gggacatagt
     6781 gagagggcaa cggcgaacaa tcaccaacgc gccctggtac gcgagaaatg ttgatatcgc
     6841 acatgacctc agcatccacc ttcagtttga gaacaaatta aatcaggcac ataccagaca
     6901 accaggagaa cactgaggag gcgtcatcaa agatatcttt ggacacgtgg cataaacaag
     6961 ccaaactatt atctaatcaa tatttttata caaattttgt atgtttccat gttatcttcg
     7021 ttgctcctgt tagttactac gtttcatagc ccttaaattg gttgcttaac gtaaaataaa
     7081 attatattgt ataaaaataa agaggaattc acctataaca aacgaaaaag taaataaata
     7141 agaacttccc catgttaaaa atgccaccac catctttaca tataagtatg tacatatgaa
     7201 tgaatcactt aggttgcttg aatattacaa tttcattaat agctaaatca catttgatgt
     7261 gttagtggaa aacggctata tatataaatt atcgaaattg tgaatatcga attgcgatag
     7321 cacaatggga aattccacca ctagattttt ggtactttta acagatcctt ttcggttttg
     7381 cgttgcgcga agtgatctga acttatcaaa gtttgtaagg taatacataa agtgaaaaag
     7441 aattaatttg ctcttgaaag gcaggccaaa ttaaaaaaaa aatatcaata ccggtgaatt
     7501 caaaaatgag agccctggta gtcatccgcc tgtcccgcgt caccgatgct acgacttcac
     7561 cggagcgtca gctggagtct tgccagcagc tctgcgccca gcgcggctgg gacgtcgtcg
     7621 gggtagcgga ggatctggac gtctccgggg cggtcgatcc gttcgaccgg aagcgcagac
     7681 cgaacctggc ccggtggcta gcgttcgagg agcaaccgtt cgacgtgatc gtggcgtacc
     7741 gggtagaccg gttgacccga tcgatccggc atctgcagca gctggtccac tgggccgagg
     7801 accacaagaa gctggtcgtc tccgcgaccg aagcgcactt cgatacgacg acgccgtttg
     7861 cggcggtcgt catcgcgctt atgggaacgg tggcgcagat ggaattagaa gcgatcaaag
     7921 agcggaaccg ttcggctgcg catttcaata tccgcgccgg gaaataccga ggatccctgc
     7981 cgccgtgggg atacctgcct acgcgcgtgg acggggagtg gcggctggtg ccggaccctg
     8041 tgcagcgaga gcgcatcctc gaggtgtatc accgcgtcgt cgacaaccac gagccgctgc
     8101 acctggtggc ccacgacctg aaccggcgtg gtgtcctgtc gccgaaggac tacttcgcgc
     8161 agctgcaagg ccgcgagccg cagggccggg agtggtcggc taccgcgctg aagcgatcga
     8221 tgatctccga ggcgatgctc gggtacgcga ctctgaacgg taagaccgtc cgagacgacg
     8281 acggagcccc gctggtgcgg gctgagccga tcctgacccg tgagcagctg gaggcgctgc
     8341 gcgccgagct cgtgaagacc tcccgggcga agcccgcggt gtctaccccg tcgctgctgc
     8401 tgcgggtgtt gttctgcgcg gtgtgcgggg agcccgcgta caagttcgcc gggggaggac
     8461 gtaagcaccc gcgctaccgc tgccgctcga tggggttccc gaagcactgc gggaacggca
     8521 cggtggcgat ggccgagtgg gacgcgttct gcgaggagca ggtgctggat ctgctcgggg
     8581 acgcggagcg tctggagaaa gtctgggtag ccggctcgga ctccgcggtc gaactcgcgg
     8641 aggtgaacgc ggagctggtg gacctgacgt cgctgatcgg ctccccggcc taccgggccg
     8701 gctctccgca gcgagaagca ctggatgccc gtattgcggc gctggccgcg cggcaagagg
     8761 agctggaggg tctagaggct cgcccgtctg gctgggagtg gcgcgagacc gggcagcggt
     8821 tcggggactg gtggcgggag caggacaccg cggcaaagaa cacctggctt cggtcgatga
     8881 acgttcggct gacgttcgac gtccgcggcg ggctgactcg cacgatcgac ttcggggatc
     8941 tgcaggagta cgagcagcat ctcaggctcg gcagcgtggt cgaacggcta cacaccggga
     9001 tgtcgtaggg tacctctaga gggcgaatcc agctctggag cagaggctct ggcagctttt
     9061 gcagcgttta tataacatga aatatatata cgcattccga tcaaagctgg gttaaccaga
     9121 tagatagata gtaacgttta actagcgcct ggcgcgttcg gttttaaaga gatttagagc
     9181 gttatcccgt gcctatagat cttatagtat agacaacgaa cgatcactca aatccaagtc
     9241 aataattcaa gaatttatgt ctgtttctgt gaaagggaaa ctaattttgt taaagaagac
     9301 ttacaatatc gtaatacttg ttcaatcgtc gtggccgata gaaatatctt acaatccgaa
     9361 agttgatgaa tggaattggt ctgcaactgg tcgccttcat tttgtaaaat gttcgcttgc
     9421 ggccgaaaaa tttcgatata tctacaattg atatacaatc tttactaaat tttgaaaaag
     9481 gaacactttg aatttcgaac tgtcaatcgt atcattagaa tttaatctaa atttaaatct
     9541 tgctaaagga aatagcaagg aacactttcg tcgtcggcta cgcattcatt gtaaaatttt
     9601 aaattttgac attccgcact ttttgataga taagcgaaga gtatttttat tacatgtatc
     9661 gcaagtattc atttcaacac acatatctat atatatatat atatatatat gttatatatt
     9721 tattcaattt tgtttaccat tgatcaattt ttcacacatg aaacaaccgc cagcattata
     9781 taattttttt atttttttaa aaaatgtgta cacatattct gaaaatgaaa aattcaatgg
     9841 ctcgagtgcc aaataaagaa atggttacaa tttaaggaaa caaatgtcct tcttgcgttt
     9901 gaaacaacta atccttttcg ccctcgcggc gtttctcgaa aagggccagg aagatgccat
     9961 cggtaagatc actgtccggc tggcaataca ggacgttctt gccaatattg ggatagtcct
    10021 tgtcgcccac attgtgccac ttgggatcca ctagtgtcga cgatgtaggt cacggtctcg
    10081 aagccgcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca
    10141 cccatctggt ccatcatgat gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg
    10201 cggcgcaccg ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg
    10261 ggacgtgcga cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg gttctcgacg
    10321 gtcacggcgg gcatgtcgac actagttcta gccagctttt gttcccttta gtgagggtta
    10381 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    10441 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    10501 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    10561 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    10621 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    10681 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    10741 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10801 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10861 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10921 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10981 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    11041 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    11101 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    11161 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    11221 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    11281 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    11341 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    11401 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    11461 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    11521 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    11581 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    11641 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    11701 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    11761 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11821 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11881 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11941 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    12001 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    12061 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    12121 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    12181 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    12241 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    12301 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    12361 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    12421 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    12481 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    12541 cgaaaagtgc cac
//
