LOCUS       sequence-174200-          94 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for bzCas13b and crRNA with both HEPN domains 
            mutated. New spacers can be cloned by digesting with BsaI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 94)
  AUTHORS   Smargon AA, Cox DB, Pyzocha NK, Zheng K, Slaymaker IM, Gootenberg 
            JS, Abudayyeh OA, Essletzbichler P, Shmakov S, Makarova KS, Koonin 
            EV, Zhang F
  TITLE     Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase 
            Differentially Regulated by Accessory Proteins Csx27 and Csx28.
  JOURNAL   Mol Cell. 2017 Feb 16;65(4):618-630.e7. doi: 
            10.1016/j.molcel.2016.12.023. Epub 2017 Jan 5.
  PUBMED    28065598
REFERENCE   2  (bases 1 to 94)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.12.023"; journalName: "Mol Cell"; date: 
            "2017-02-16- 16"; volume: "65"; issue: "4"; pages: "618-630.e7"
COMMENT     Sequence Label: pBZCas13b-HEPN
FEATURES             Location/Qualifiers
     source          1..94
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gttggaactg ctctcatttt ggagggtaat cacaacgaga ccatggcgga aggtctcagt
       61 tggaactgct ctcattttgg agggtaatca caac
//
