LOCUS       sequence-174202-       10025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked 
            alleles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Voutev R, Mann RS
  TITLE     Bxb1 phage recombinase assists genome engineering in Drosophila 
            melanogaster.
  JOURNAL   Biotechniques. 2017 Jan 1;62(1):37-38. doi: 10.2144/000114494.
  PUBMED    28118814
REFERENCE   2  (bases 1 to 10025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2017-01-1- 1"; volume: "62"; issue: "1"; 
            pages: "37-38"
COMMENT     Sequence Label: pRVV651
FEATURES             Location/Qualifiers
     source          1..10025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1360..1362,1363..1365,1366..2079)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1405..1426)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1666..1685)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2013..2034
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2101..2222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2112..2131)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2166..2185
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    2329..2376
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     promoter        complement(2580..2598)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2619..2635)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2619..2635)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2632..2654)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2643..2659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2667..2673,2674..2691,2692..2697))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2712..2733
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2850..2867)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3021..3609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3101..3120)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3780..4571,4572..4640))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4403..4422
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4641..4745)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4771..5226)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4908..4929)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5120..5139
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5353..5375
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5367..5384
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5368..5384
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5391..5410
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5391..5409
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5443..5461)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5913..5984,6386..6659,6734..7388,7450..7765,7969..8100,
                     8171..8785)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    9613..9660
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 gtagagaaga cagtgctatt tcaaaagcaa actaacaagg gtcttaaatt ccaaaacacc
       61 aatcctaaca agccttggac ttttgtaagt ttagatcaaa ggtggcattg cattcaatgt
      121 catggtaaga agtaggtcgt ctaggtagaa atcctcattc agccggtcaa gtcagtacga
      181 gaaaggtctc aatttgaaat tgtcttaaaa atattttatt gttttgtact gtggtgagtt
      241 taaacgaaaa acacaaaaaa aaagtgatac acagaaatca taaaaaattt taatacaagg
      301 tattcgtacg tatcaaaaac atttcggcac aatttttttt ctctgtacta aagtgttacg
      361 aacactacgg tattttttag tgattttcaa cggacaccga aggtatataa acagcgttcg
      421 cgaacggtcg ccttcaaaac caattgacat ttgcagcagc aagtacaagt agaaagtaaa
      481 gcgcaatcag cgaaaaattt atacttaatt gttggtgatt aaagtacaat taaaagaaca
      541 ttctcgaaag tcacaagaaa cgtaagtttt taactcgctg ttaccaatta gtaataagag
      601 caacaagacg ttgagtaatt tcaagaaaaa ctgcatttca aggtctttgt tcggccattt
      661 ttttttattc aacgctctac gtaattacaa aataagaaat tggcagccac gcatcttgtt
      721 ttcccaatga attggcatca aaacgcaaac aaatctataa ataaaacttg cgtgttgatt
      781 ttcgccaaga tttattggca aattgtgaaa ttcgcagtga cgcatttgaa aattcgagaa
      841 atcacgaacg cactcgagca tttgtgtgca tgttattagt tagttgttta gttaattgaa
      901 gtattttacc aacgaaatcc acttattttt agctgaaata gagtaggttg cttaaacaaa
      961 gccacgtctg aaaatttctt attgcttgta gttgtgacgt caccatatac acacaaaata
     1021 atgtgtatgc atgcatttca gctgtgtata tatacatgca cacactcgca acacgaaaac
     1081 gatgacgaag caacggaaca aaggtttctc aactaccctt tgttccctgt ttcttcgctt
     1141 tcctttgttc caatattcgt agagggttaa taggggtttc tcaacaaagt tggcgtcgat
     1201 aaataagttt cccattttta ttccccagcc aggaagttag tttcaatagt tttgtaattt
     1261 caacgaaact catttgattt cgtactaatt ttccacatct ctattttctg cccgcagaat
     1321 aatccaaggg cggatccccc gggctgcagg aattcaaaaa tggtgagcaa gggcgaggag
     1381 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1441 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1501 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1561 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1621 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1681 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1741 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1801 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggc caacttcaag
     1861 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1921 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1981 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2041 gccgggatca ctctcggcat ggacgagctg tacaagtagg gtacctctag acagacatga
     2101 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     2161 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2221 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     2281 tttaaagcga agttcctatt cttcaaaagg tataggaact tcaccggtgg tttgtctggt
     2341 caaccaccgc ggtctcagtg gtgtacggta caaaccggcg cgccgctagc catatgggga
     2401 ttggtggcga cgactcctgg agcccgtcag tatcggcgga attacagctg agcgccggtc
     2461 gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg ccatgtctgc
     2521 ccgtatttcg cgtaaggaaa tccctcgagg gtacctctag ttctagccag cttttgttcc
     2581 ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga
     2641 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2701 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2761 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2821 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2881 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2941 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3001 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3061 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3121 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3181 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3241 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3301 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3361 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3421 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3481 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3541 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3601 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3661 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3721 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3781 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3841 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3901 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3961 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4021 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4081 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4141 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4201 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4261 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4321 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4381 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4441 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4501 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4561 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4621 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4681 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4741 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     4801 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     4861 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     4921 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     4981 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     5041 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     5101 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     5161 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     5221 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     5281 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     5341 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
     5401 cactataggg cgaattgggt acgtaccggg cccctagtat gtatgtaagt taataaaacc
     5461 catttttgcg gaaagtagat aaaaaaaaca tttttttttt ttactgcact ggatatcatt
     5521 gaacttatct gatcagtttt aaatttactt cgatccaagg gtatttgatg taccaggttc
     5581 tttcgattac ctctcactca aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt
     5641 ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg tccgctatct ctttcgccac
     5701 cgtttgtagc gttacgtagc gtcaatgtcc gccttcagtt gcattttgtc agcggtttcg
     5761 tgacgaagct ccaagcggtt tacgccatca attaaacaca aagtgctgtg ccaaaactcc
     5821 tctcgcttct tatttttgtt tgttttttga gtgattgggg tggtgattgg ttttgggtgg
     5881 gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca agaggatcag gagctattaa
     5941 ttcgcggagg cagcaaacac ccatctgccg agcatctgaa caatgtgagt agtacatgtg
     6001 catacatctt aagttcactt gatctatagg aactgcgatt gcaacatcaa attgtctgcg
     6061 gcgtgagaac tgcgacccac aaaaatccca aaccgcaatt gcacaaacaa atagtgacac
     6121 gaaacagatt attctggtag ctgttctcgc tatataagac aatttttgag atcatatcat
     6181 gatcaagaca tctaaaggca ttcattttcg actatattct tttttacaaa aaatataaca
     6241 accagatatt ttaagctgat cctagatgca caaaaaataa ataaaagtat aaacctactt
     6301 cgtaggatac ttcggggtac tttttgttcg gggttagatg agcataacgc ttgtagttga
     6361 tatttgagat cccctatcat tgcagggtga cagcggagcg gcttcgcaga gctgcattaa
     6421 ccagggcttc gggcaggcca aaaactacgg cacgctccgg ccacccagtc cgccggagga
     6481 ctccggttca gggagcggcc aactagccga gaacctcacc tatgcctggc acaatatgga
     6541 catctttggg gcggtcaatc agccgggctc cggatggcgg cagctggtca accggacacg
     6601 cggactattc tgcaacgagc gacacatacc ggcgcccagg aaacatttgc tcaagaacgg
     6661 tgagtttcta ttcgcagtcg gctgatctgt gtgaaatctt aataaagggt ccaattacca
     6721 atttgaaact cagtttgcgg cgtggcctat ccgggcgaac ttttggccgt gatgggcagt
     6781 tccggtgccg gaaagacgac cctgctgaat gcccttgcct ttcgatcgcc gcagggcatc
     6841 caagtatcgc catccgggat gcgactgctc aatggccaac ctgtggacgc caaggagatg
     6901 caggccaggt gcgcctatgt ccagcaggat gacctcttta tcggctccct aacggccagg
     6961 gaacacctga ttttccaagc catggtgcgg atgccacgac atctgaccta tcggcagcga
     7021 gtggcccgcg tggatcaggt gatccaggag ctttcgctca gcaaatgtca gcacacgatc
     7081 atcggtgtgc ccggcagggt gaaaggtctg tccggcggag aaaggaagcg tctggcattc
     7141 gcctccgagg ctctaaccga tccgccgctt ctgatctgcg atgagcccac ctccggactg
     7201 gactccttta ccgcccacag cgtcgtccag gtgctgaaga agctgtcgca gaagggcaag
     7261 accgtcatcc tgaccattca tcagccgtct tccgagctgt ttgagctctt tgacaagatc
     7321 cttctgatgg ccgagggcag ggtagctttc ttgggcactc ccagcgaagc cgtcgacttc
     7381 ttttcctagt gagttcgatg tgtttattaa gggtatctag tattacataa catctcaact
     7441 cctatccagc gtgggtgccc agtgtcctac caactacaat ccggcggact tttacgtaca
     7501 ggtgttggcc gttgtgcccg gacgggagat cgagtcccgt gatcggatcg ccaagatatg
     7561 cgacaatttt gccattagca aagtagcccg ggatatggag cagttgttgg ccaccaaaaa
     7621 tctggagaag ccactggagc agccggagaa tgggtacacc tacaaggcca cctggttcat
     7681 gcagttccgg gcggtcctgt ggcgatcctg gctgtcggtg ctcaaggaac cactcctcgt
     7741 aaaagtgcga cttattcaga caacggtgag tggttccagt ggaaacaaat gatataacgc
     7801 ttacaattct tggaaacaaa ttcgctagat tttagataga attgcctgat tccacaccct
     7861 tcttagtttt tttcaatgag atgtatagtt tatagttttg cagaagataa ataaatttca
     7921 tttaactcgc gaatattaat gagatgcgag taacatttta atttgcagat ggttgccatc
     7981 ttgattggcc tcatcttttt gggccaacaa ctcacgcaag tgggtgtgat gaatatcaac
     8041 ggagccatct tcctcttcct gaccaacatg acctttcaaa acgtctttgc cacgataaat
     8101 gtaagtcatg tttagaatac atttgcattt caataattta ctaactttct aatgaatcga
     8161 ttcgatttag gtgttcacct cagagctgcc agtttttatg agggaggccc gaagtcgact
     8221 ttatcgctgt gacacatact ttctgggcaa aacgattgcc gaattgccgc tttttctcac
     8281 agtgccactg gtcttcacgg cgattgccta tccgatgatc ggactgcggg ccggagtgct
     8341 gcacttcttc aactgcctgg cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg
     8401 atatctaata tcctgcgcca gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt
     8461 tatcatacca ttcctgctct ttggcggctt cttcttgaac tcgggctcgg tgccagtata
     8521 cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc tgctgattaa
     8581 ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc
     8641 cagttcgggc aaggtcatcc tggagacgct taacttctcc gccgccgatc tgccgctgga
     8701 ctacgtgggt ctggccattc tcatcgtgag cttccgggtg ctcgcatatc tggctctaag
     8761 acttcgggcc cgacgcaagg agtagccgac atatatccga aataactgct tgtttttttt
     8821 ttttaccatt attaccatcg tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt
     8881 atatttgtgc caataaaaac aagatatgac ctatagaata caagtatttc cccttcgaac
     8941 atccccacaa gtagactttg gatttgtctt ctaaccaaaa gacttacaca cctgcatacc
     9001 ttacatcaaa aactcgttta tcgctacata aaacaccggg atatattttt tatatacata
     9061 cttttcaaat cgcgcgccct cttcataatt cacctccacc acaccacgtt tcgtagttgc
     9121 tctttcgctg tctcccaccc gctctccgca acacattcac cttttgttcg acgaccttgg
     9181 agcgactgtc gttagttccg cgcgattcgg ttcgctcaaa tggttccgag tggttcattt
     9241 cgtctcaata gaaattagta ataaatattt gtatgtacaa tttatttgct ccaatatatt
     9301 tgtatatatt tccctcacag ctatatttat tctaatttaa tattatgact ttttaaggta
     9361 attttttgtg acctgttcgg agtgattagc gttacaattt gaactgaaag tgacatccag
     9421 tgtttgttcc ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt gttgtttata
     9481 tatatcaaaa ataacaacta taataataag aatacattta atttagaaaa tgcttggatt
     9541 tcactggaac taggctagat ttaaatgcat gccctgcagg aagcttccta ggaggcctag
     9601 atctgcggcc gcggtttgta ccgtacacca ctgagaccgc ggtggttgac cagacaaacc
     9661 ttaattaaga agttcctatt cttcaaaagg tataggaact tctctagccc ggcttgctgt
     9721 tcttcgcgtt caaaatctcc agctccattt tgctttcggt gcgcttgcaa tcagtactgt
     9781 ccaaaatcga aaatcgccga accgtagtgt gaccgtgcgg ggctctgcga aaataaactt
     9841 ttttaggtat atggccacac acggggaaag cacagtggat tatatgtttt aatattataa
     9901 tatgcaggtt ttcattactt atccagatgt aagcccactt aaagcgattt aacaattatt
     9961 tgccgaaaga gtataaacaa atttcactta aaaatggatt aagaaaagct tgtgtaagat
    10021 tatgc
//
