LOCUS       sequence-174211-        9552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses C151S and C288S double mutant human Keap1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Parvez S, Fu Y, Li J, Long MJ, Lin H, Lee DK, Hu GS, Aye Y
  TITLE     Substoichiometric Hydroxynonenylation of a Single Protein 
            Recapitulates Whole-Cell-Stimulated Antioxidant Response.
  JOURNAL   J Am Chem Soc. 2014 Dec 31.
  PUBMED    25544059
REFERENCE   2  (bases 1 to 9552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2014 Dec 31."
COMMENT     Sequence Label: pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S
FEATURES             Location/Qualifiers
     source          1..9552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     140..159
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(256..572)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(612..634)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     734..753
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     961..978
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     962..978
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3582..3602)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(3609..4499)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             complement(4503..4520)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(4530..4547)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(4560..4568,4569..4571,4572..5145)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4733..4752)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4962..4979
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(5188..5865)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(5250..5273)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5448..5467)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5665..5685
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5717..5735
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(5890..6093)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5919..5939)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6094..6397
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(6518..6537)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6519..6537)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6549..6567)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6550..6567)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6587..6603)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6587..6603)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6600..6622)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6611..6627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6635..6641,6642..6659,6660..6665))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6680..6701
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6818..6835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6989..7577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7069..7088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7748..8539,8540..8608))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8371..8390
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8609..8713)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8801..8882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8826..8845
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(9112..9552,1..159))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9195..9215)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gaagagttct tgcagctcgg tgacccgctc gatgtggcgg tccgggtcga cggtgtggcg
       61 cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt acggcccggg ggacgtcgtc
      121 gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg gaaggtcgtc tccttgtgag
      181 gggtcagggg cgtgggtcag gggatggtgg cggcaccggt cgtggcggcc gacctgcagg
      241 catgcaagct agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
      301 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
      361 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
      421 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
      481 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
      541 gcctgctggg gagcctgggg actttccaca ccaatctttc gtctcgcgcg tttcggtgat
      601 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      661 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      721 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
      781 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
      841 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
      901 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gacacgacgt
      961 tgtaaaacga cggccagtgc caagctagcg gccgcataca aaaaaccaac acacagatcc
     1021 aatgaaaata aaagatcctt tattaagctt agctgaaaac ttctggctca catagcttct
     1081 gtcaccacca atgagatgcc acatgcgctg actacttggg tcacttttaa tttttatgtt
     1141 gctgattgta tgtgtgcatc tcctgtcttg taagcattaa acaaatgaag acatatgcag
     1201 agctgtgaaa ttcactctgc actgaataga caaaaagggt tacatagcat aacaatgagc
     1261 agttggtatc cacgtggttc tcagcattat aaactctggg gaagatgcta cttcgtaacc
     1321 tcaaggaaat aagtaagcaa ggcagaagga acacttaaac aagtattatt caacagacat
     1381 gtttacacta aataattaag gttatattta gggtttaaac tcatccctga atatccttag
     1441 taacaacctg gaggaaaaac tggggtgaat gagaacttaa gttttccaag gattatggtt
     1501 caaatcctct gaaatctata acaggttaaa aaaatgagaa ctcaactttc tagagcaata
     1561 actcttctcg agggcactag cacaaggcgg tagtgatttt cacttttaat atattacata
     1621 taatactaca aaaagactga agttttaagg aaaatccatt attattaaaa gtttagtact
     1681 cttgagtctg gactttctga tcaacaggta cagttctgct ggtcaatctg cttccggcag
     1741 ggctccatgg tgacagccac gcccacccca ctccggcccg atgtcattcg ggtcacctcg
     1801 ctccaggtgt ctgtatctgg gtcgtaacac tccacactgt ccaggaacgt gtgaccatca
     1861 tagcctccaa ggacgtagat tctcccctgg tggacagtga tccccagggc acttcgccgg
     1921 tgcttcatgg gggctacgaa agtccacgtc tctgtttcca catcgtagcg ctccacgctg
     1981 ttcagctggt cctgaccatc atagccccca gcagcataga tacagttgtg caggacgcag
     2041 acgcctgccc cgcttcggat ggtgttcatt gctgtgatca ttcgccactc gttcctctct
     2101 gggtagtaac actcagctga attaaggcgg tttgtcccgt caaagccccc cacggcataa
     2161 aggagacgat tgaggacagc cacgcccacc ccgatccttc gtgtcagcat tggggccacc
     2221 aagtgccact catcccgctc tggctcatac ctctccacac tgttgtggtg gatgcagccg
     2281 tgggagccgc cgacggcata gatgtggcca tcgatgaccc ccaccccgat gcggttacgg
     2341 ggcacgctca tgggggcgca gggcgaccac tgattggtca tggggttgta acagtccagg
     2401 gcgctggagt cggtgttgcc gtcgggcgag ttgttcctgc cgcccacggc gtacaacagc
     2461 ccgcccacca cgcagccggc caggccgctc cgcggcacct gcaggtccgc caaccggagc
     2521 caggtgccgt cactggggtt gtaagcctcc aggtagctga gcgactgtcg gaagtagccg
     2581 cccgcggtgt agatcaggcg gcccaccttg ggcgcccggc agggcatcac ctgcgtgggc
     2641 ttgtgcaggg tgagctcctc gaagatcttg accaggtagt ccttgcagcg ggagtcggac
     2701 tgcaggatct cgctcttctg cagctgcatc tgcaggaagt tcggcgtcaa cgagtggcag
     2761 cgcactgccc gcagcagcgc ctggacgtag aaccgtcgct gttcgcagtc gtacttgacc
     2821 cagttgatgc aggcgtggaa gacctcggac tcgcagcgca cgttcaggtc gtcccggctg
     2881 atgagggtca ccagttggca gtgggacagg ttgaagaact cctcttgctt ggccacctcc
     2941 ccaaaatgca tgtagatgta ctcccgggca cgctggtgca actccacaca gccaatctgc
     3001 tcagcgaagt tggcgatgcc gatggcattg ctggggtcca gctgctgcac caggaagtca
     3061 ctgcaggcac ggacaacgct gtcgatctgg tacatgacag caccgttcat gacgtggagg
     3121 acactcttct cgcccatgga gatggaggcc gtgtaggcga attcaatgag gcgctccatg
     3181 accttggggt ggataccctc aatggacacc acctccatgc cctgctcccg cagcccgttg
     3241 gtgaacatgg ccttgaagac agggctggat gaggccagca ccaccttgtg ggccatgaac
     3301 tgggcggccg gtgcatcctg gtacttgacc tgcagtgtga cgtcacacag ctgctggctg
     3361 agccgcagct cgttcatgat gccaaaggcc tgcttggtat gatcctccag ggtgtagctg
     3421 aaggtgcggt tgccatgctg ggagggcgtc acctccgcct tgcactcagt ggaggcgtac
     3481 atcaccgcgt cccctgcccc ctcagggcac tgtgactgca ggggcaggaa tcggcagcag
     3541 gccccagccc cgctaggcct gggatctggc tgcatccctg agccctggaa atacaagttt
     3601 tctccggagc cggaaatctc gagcgtcgac agccagcgcg cgatctcgct gccgatcagg
     3661 tccgggttgt cttcttgcag cagattcaga cccgggccga tgtccacagc cttgcagtta
     3721 ggcaggcttt tggccaggcg agcggcttcg gccggtggga tcagaacgcc tggggtgccc
     3781 cagaacagca gcttcgggac aggggactgg tgcagccagt ccatgtattc ttcgaccagc
     3841 gcgacgatgt tcgctggctc accggcgatt ggcagctcgt ttgggaagcg ccacagtggc
     3901 tcgcggtcaa cagggttcag gaacggctcg cggtaatggt ccatctcgac ttcagtcagc
     3961 gggcggacga cacccatcgg cagcgtaccc tcgataaaaa cgttctgatc gatgatcagc
     4021 ttgcggccga cgtcggtggt gcggaaggcc tggaaggtct cgcgggcaaa ttctggccat
     4081 tcgtcccagg tcgggatagg gcggatgaac tccataaatg caataccttt gacgcgctct
     4141 ggattgcgct tggcccagtg gaaacccaga gcggagcccc agtcgtgaat gaccaggacg
     4201 acctcttcca gacccagggc ttcgatgaag gcatccatga agcggacgtg gtcgtcgaag
     4261 aaataaccca ggtctggttt gtcggatttg cccataccga tcaggtctgg agcaatgcag
     4321 cgatgggtcg gtgcaacatg cgggatgatg ttgcgccaca cgtaggagga ggtcgggtta
     4381 ccgtgcagga acagcacagg ggtgccatcg cgcggaccaa catcgacgta gtgcatgcgc
     4441 tcgcccagga cttccacata atgggggtcg aatggaaagc cagtaccgat ttctgccata
     4501 tggctaccac gaggcaccag gccgctgctg tgatgatgat gatgatggct gctgcccatg
     4561 gttgtggcca tattatcatc gtgtttttca aaggaaaacc acgtccccgt ggttcggggg
     4621 gcctagacgt ttttttaacc tcgactaaac acatgtaaag catgtgtacc gaggccccag
     4681 atcagatccc atacaatggg gtaccttctg ggcatccttc agccccttgt tgaatacgct
     4741 tgaggagagc catttgactc tttccacaac tatccaactc acaacgtggc actggggttg
     4801 tgccgccttt gcaggtgtat cttatacacg tggcttttgg ccgcagaggc acctgtcgcc
     4861 aggtgggggg ttccgctgcc tgcaaagggt cgctacagac gttgtttgtc ttcaagaagc
     4921 ttccagagga actgcttcct tcacgacatt caacagacct tgcattcctt tggcgagagg
     4981 ggaaagaccc ctaggaatgc tcgtcaagaa gacagggcca ggtttccggg ccctcacatt
     5041 gccaaaagac ggcaatatgg tggaaaataa catatagaca aacgcacacc ggccttattc
     5101 caagcggctt cggccagtaa cgttaggggg ggggagggag aggggcggat cccgggcccg
     5161 cggtaccgtc gactgcagaa ttggccgcta caggaacagg tggtggcggc cctcggcgcg
     5221 ctcgtactgc tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttggagtc
     5281 cacgtagtag tagccgggca gctgcacggg cttcttggcc atgtagatgg acttgaactc
     5341 caccaggtag tggccgccgt ccttcagctt cagggccttg tggatctcgc ccttcagcac
     5401 gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag cccatagtct tcttctgcat
     5461 tacggggccg tcggagggga agttcacgcc gatgaacttc accttgtaga tgaaggagcc
     5521 gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac
     5581 gcgctcccac ttgaagccct cggggaagga cagcttcttg tagtcgggga tgtcggcggg
     5641 gtgcttcacg tacaccttgg agccgtactg gaactggggg gacaggatgt cccaggcgaa
     5701 gggcaggggg ccgcccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
     5761 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgcgcac
     5821 cttgaagcgc atgaactcct tgatgacgtc ctcggaggag gccatggtgg atccacggtt
     5881 cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     5941 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     6001 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     6061 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     6121 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     6181 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     6241 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     6301 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     6361 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     6421 aactaatgac cccgtaattg attactatta ataactaaga tctggtacct tgaattcatg
     6481 cttctcctcc ctttagtgag ggtaattctc tctctctccc tatagtgagt cgtattaatt
     6541 ccttctcttc tatagtgtca cctaaatcgt tgcaattcgt aatcatgtca tagctgtttc
     6601 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6661 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6721 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6781 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6841 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6901 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6961 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7021 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7081 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7141 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7201 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7261 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7321 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7381 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7441 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7501 gcaaaaaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7561 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7621 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7681 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7741 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7801 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7861 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7921 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7981 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8041 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8101 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8161 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8221 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8281 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8341 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8401 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8461 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8521 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8581 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8641 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8701 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     8761 tcatgacatt aacctataaa aataggcgta tcacgagatt gcagtgaaaa aaatgcttta
     8821 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     8881 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     8941 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagagtcgg
     9001 tgggcctcgg gggcgggtgc ggggtcggcg gggccgcccc gggtggcttc ggtcggagcc
     9061 atggggtcgt gcgctccttt cggtcgggcg ctgcgggtcg tggggcgggc gtcaggcacc
     9121 gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac
     9181 ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct ccaggaaggc
     9241 gggcaccccg gcgcgctcgg ccgcctccac tccggggagc acgacggcgc tgcccagacc
     9301 cttgccctgg tggtcgggcg agacgccgac ggtggccagg aaccacgcgg gctccttggg
     9361 ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg gccagccggg aaccgctcaa
     9421 ctcggccatg cgcgggccga tctcggcgaa caccgccccc gcttcgacgc tctccggcgt
     9481 ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac accttgccga tgtcgagccc
     9541 gacgcgcgtg ag
//
