LOCUS       sequence-174223-        5502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GGvT, a tandem homodimer green fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Wiens M, Shen Y, Li X, Salem M, Smisdom N, Zhang W, Brown A, 
            Campbell RE
  TITLE     A tandem green-red heterodimeric fluorescent protein with high FRET 
            efficiency.
  JOURNAL   Chembiochem. 2016 Oct 26. doi: 10.1002/cbic.201600492.
  PUBMED    27781394
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2016 Oct 26. doi: 10.1002/cbic.201600492."
COMMENT     Sequence Label: pBad-HisB-GGvT
FEATURES             Location/Qualifiers
     source          1..5502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(55..933)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        960..1244
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1167..1186
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1290..1307
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1311..1343
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(1347..1355,1356..1370)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(2862..2879)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3032..3118
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3210..3237
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3256..3347
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3348..3416,3417..4208)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3566..3585)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4379..4967
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4868..4887
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5121..5138
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5153..5293
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5219..5238)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5379..5401
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga
       61 caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct
      121 ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt
      181 gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat
      241 acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga
      301 cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc
      361 tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg
      421 gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc
      481 cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc
      541 gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga
      601 cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata
      661 ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa
      721 aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg
      781 aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag
      841 ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc
      901 cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga
      961 agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt
     1021 ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa
     1081 agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga
     1141 ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga
     1201 tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt tttttgggct
     1261 aacaggagga attaaccatg gggggttctc atcatcatca tcatcatggt atggctagca
     1321 tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag gatccgagct
     1381 cgagaatggt gagcaagggc gaggaagtga tcaaagagtt catgcggttc aaagtgcgga
     1441 tggaaggcag catgaacggc cacgagttcg agatcgaggg cgagggcgaa ggcagacctt
     1501 acgagggaac ccagaccgcc aagctgaaag tgaccaaggg cggacccctg cccttcgcct
     1561 gggatatcct gagccccctg ctgatgtacg gcagcaagat gtacgtgaag caccccgccg
     1621 acatccccga ctacaagaag ctgagcttcc ccgagggctt caaatgggtg cgcgtgatga
     1681 acttcgagga cggcggactg gtgaccgcca cacaggatag ttctctgcag gacggcaccc
     1741 tgatctacga agtgaagatg cggggcacca acttcccccc cgatggcccc gtgatgcaga
     1801 aaaagaccat gggctgggag gccagcaccg agagactgta ccctagagat ggcgtgctga
     1861 agggcgagat ccaccaggcc ctgaagctga aggatggcgg ccactacctg gtggagttca
     1921 agaccatcta catggccaag aaacccgtgc agctgcccgg ctactactac gtggacacca
     1981 agctggacat caccagccac aacgaggact acacccgtgt ggaacagtac gagcggagcg
     2041 agggccggca ccacctgttt ctgtacgaca tggacgagct gtacaagggc agcaccggca
     2101 gcggcagctc cggccccatg gtgtctaaag gcgaagaagt gatcaaagag tttatgcgct
     2161 ttaaagtgcg catggaaggc tccatgaatg ggcatgagtt tgagattgaa ggggaggggg
     2221 agggcaggcc ctatgagggc acacagacag ctaaactgaa agtgacaaaa gggggccctc
     2281 tgccttttgc ttgggacatc ctgtctccac tgctgatgta tgggtctaag atgtatgtga
     2341 aacatcctgc cgatattcca gattataaga agctgtcctt tccagagggg tttaaatggg
     2401 tgcgcgtgat gaattttgaa gatgggggcc tggttacagc tacccaggac agtagcctgc
     2461 aggatggaac actgatctat gaagtgaaaa tgagagggac caattttccc cctgacggac
     2521 ctgtgatgca gaaaaaaaca atgggatggg aagcctctac cgagcggctg tacccaaggg
     2581 acggggtgct gaaaggggaa attcatcagg ctctgaaact gaaagacggg gggcattatc
     2641 tggtggagtt taaaacaatc tatatggcta aaaagcctgt gcagctgcct gggtactatt
     2701 atgtggatac aaaactggat atcacctctc acaatgagga ttatacaaga gtggaacagt
     2761 atgagagatc cgaggggcgc catcatctgt ttctgtatga tatggatgaa ctgtataagt
     2821 gataagcttg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
     2881 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
     2941 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
     3001 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     3061 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     3121 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
     3181 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
     3241 gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3301 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3361 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3421 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3481 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3541 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     3601 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3661 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3721 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3781 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3841 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3901 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3961 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4021 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4081 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4141 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4201 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4261 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4321 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4381 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaacaacca ccgctaccag
     4441 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4501 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4561 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4621 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4681 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4741 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4801 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4861 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4921 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4981 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5041 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5101 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     5161 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     5221 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     5281 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5341 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5401 gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa
     5461 gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg at
//
