LOCUS       sequence-174227-        9553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses his-tagged C288S human Keap1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Parvez S, Fu Y, Li J, Long MJ, Lin H, Lee DK, Hu GS, Aye Y
  TITLE     Substoichiometric Hydroxynonenylation of a Single Protein 
            Recapitulates Whole-Cell-Stimulated Antioxidant Response.
  JOURNAL   J Am Chem Soc. 2014 Dec 31.
  PUBMED    25544059
REFERENCE   2  (bases 1 to 9553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2014 Dec 31."
COMMENT     Sequence Label: pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S
FEATURES             Location/Qualifiers
     source          1..9553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     107..126
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(223..539)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      237..372
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(579..601)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     701..720
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     928..945
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     929..945
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3549..3569)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(3576..4466)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             complement(4470..4487)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(4497..4514)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(4527..4535,4536..4538,4539..5113)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4700..4719)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4929..4946
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(5156..5833)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(5218..5241)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5416..5435)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5633..5653
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5685..5703
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(5858..6061)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5887..5907)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6062..6365
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(6486..6505)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6487..6505)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6517..6535)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6518..6535)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6555..6571)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6555..6571)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6568..6590)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6579..6595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6603..6609,6610..6627,6628..6633))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6648..6669
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6786..6803)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6957..7545)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7037..7056)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7716..8507,8508..8576))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8339..8358
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8577..8681)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8769..8850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8794..8813
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(9080..9553,1..126))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9163..9183)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gtggcggtcc gggtcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag
       61 ggtgcgtacg gcccggggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc
      121 ggtcatggaa ggtcgtctcc ttgtgagggg tcaggggcgt gggtcagggg atggtggcgg
      181 caccggtcgt ggcggccgac ctgcaggcat gcaagctagc tttttgcaaa agcctaggcc
      241 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
      301 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
      361 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
      421 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
      481 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacca
      541 atctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
      601 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
      661 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
      721 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
      781 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
      841 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
      901 acgccagggt tttcccagac acgacgttgt aaaacgacgg ccagtgccaa gctagcggcc
      961 gcatacaaaa aaccaacaca cagatccaat gaaaataaaa gatcctttat taagcttagc
     1021 tgaaaacttc tggctcacat agcttctgtc accaccaatg agatgccaca tgcgctgact
     1081 acttgggtca cttttaattt ttatgttgct gattgtatgt gtgcatctcc tgtcttgtaa
     1141 gcattaaaca aatgaagaca tatgcagagc tgtgaaattc actctgcact gaatagacaa
     1201 aaagggttac atagcataac aatgagcagt tggtatccac gtggttctca gcattataaa
     1261 ctctggggaa gatgctactt cgtaacctca aggaaataag taagcaaggc agaaggaaca
     1321 cttaaacaag tattattcaa cagacatgtt tacactaaat aattaaggtt atatttaggg
     1381 tttaaactca tccctgaata tccttagtaa caacctggag gaaaaactgg ggtgaatgag
     1441 aacttaagtt ttccaaggat tatggttcaa atcctctgaa atctataaca ggttaaaaaa
     1501 atgagaactc aactttctag agcaataact cttctcgagg gcactagcac aaggcggtag
     1561 tgattttcac ttttaatata ttacatataa tactacaaaa agactgaagt tttaaggaaa
     1621 atccattatt attaaaagtt tagtactctt gagtctggac tttctgatca acaggtacag
     1681 ttctgctggt caatctgctt ccggcagggc tccatggtga cagccacgcc caccccactc
     1741 cggcccgatg tcattcgggt cacctcgctc caggtgtctg tatctgggtc gtaacactcc
     1801 acactgtcca ggaacgtgtg accatcatag cctccaagga cgtagattct cccctggtgg
     1861 acagtgatcc ccagggcact tcgccggtgc ttcatggggg ctacgaaagt ccacgtctct
     1921 gtttccacat cgtagcgctc cacgctgttc agctggtcct gaccatcata gcccccagca
     1981 gcatagatac agttgtgcag gacgcagacg cctgccccgc ttcggatggt gttcattgct
     2041 gtgatcattc gccactcgtt cctctctggg tagtaacact cagctgaatt aaggcggttt
     2101 gtcccgtcaa agccccccac ggcataaagg agacgattga ggacagccac gcccaccccg
     2161 atccttcgtg tcagcattgg ggccaccaag tgccactcat cccgctctgg ctcatacctc
     2221 tccacactgt tgtggtggat gcagccgtgg gagccgccga cggcatagat gtggccatcg
     2281 atgaccccca ccccgatgcg gttacggggc acgctcatgg gggcgcaggg cgaccactga
     2341 ttggtcatgg ggttgtaaca gtccagggcg ctggagtcgg tgttgccgtc gggcgagttg
     2401 ttcctgccgc ccacggcgta caacagcccg cccaccacgc agccggccag gccgctccgc
     2461 ggcacctgca ggtccgccaa ccggagccag gtgccgtcac tggggttgta agcctccagg
     2521 tagctgagcg actgtcggaa gtagccgccc gcggtgtaga tcaggcggcc caccttgggc
     2581 gcccggcagg gcatcacctg cgtgggcttg tgcagggtga gctcctcgaa gatcttgacc
     2641 aggtagtcct tgcagcggga gtcggactgc aggatctcgc tcttctgcag ctgcatctgc
     2701 aggaagttcg gcgtcaacga gtggcagcgc actgcccgca gcagcgcctg gacgtagaac
     2761 cgtcgctgtt cgcagtcgta cttgacccag ttgatgcagg cgtggaagac ctcggactcg
     2821 cagcgcacgt tcaggtcgtc ccggctgatg agggtcacca gttggcagtg ggacaggttg
     2881 aagaactcct cttgcttggc cacctcccca aaatgcatgt agatgtactc ccgggcacgc
     2941 tggtgcaact ccacacagcc aatctgctca gcgaagttgg cgatgccgat ggcattgctg
     3001 gggtccagct gctgcaccag gaagtcactg caggcacgga caacgctgtc gatctggtac
     3061 atgacagcac cgttcatgac gtggaggaca cacttctcgc ccatggagat ggaggccgtg
     3121 taggcgaatt caatgaggcg ctccatgacc ttggggtgga taccctcaat ggacaccacc
     3181 tccatgccct gctcccgcag cccgttggtg aacatggcct tgaagacagg gctggatgag
     3241 gccagcacca ccttgtgggc catgaactgg gcggccggtg catcctggta cttgacctgc
     3301 agtgtgacgt cacacagctg ctggctgagc cgcagctcgt tcatgatgcc aaaggcctgc
     3361 ttggtatgat cctccagggt gtagctgaag gtgcggttgc catgctggga gggcgtcacc
     3421 tccgccttgc actcagtgga ggcgtacatc accgcgtccc ctgccccctc agggcactgt
     3481 gactgcaggg gcaggaatcg gcagcaggcc ccagccccgc taggcctggg atctggctgc
     3541 atccctgagc cctggaaata caagttttct ccggagccgg aaatctcgag cgtcgacagc
     3601 cagcgcgcga tctcgctgcc gatcaggtcc gggttgtctt cttgcagcag attcagaccc
     3661 gggccgatgt ccacagcctt gcagttaggc aggcttttgg ccaggcgagc ggcttcggcc
     3721 ggtgggatca gaacgcctgg ggtgccccag aacagcagct tcgggacagg ggactggtgc
     3781 agccagtcca tgtattcttc gaccagcgcg acgatgttcg ctggctcacc ggcgattggc
     3841 agctcgtttg ggaagcgcca cagtggctcg cggtcaacag ggttcaggaa cggctcgcgg
     3901 taatggtcca tctcgacttc agtcagcggg cggacgacac ccatcggcag cgtaccctcg
     3961 ataaaaacgt tctgatcgat gatcagcttg cggccgacgt cggtggtgcg gaaggcctgg
     4021 aaggtctcgc gggcaaattc tggccattcg tcccaggtcg ggatagggcg gatgaactcc
     4081 ataaatgcaa tacctttgac gcgctctgga ttgcgcttgg cccagtggaa acccagagcg
     4141 gagccccagt cgtgaatgac caggacgacc tcttccagac ccagggcttc gatgaaggca
     4201 tccatgaagc ggacgtggtc gtcgaagaaa taacccaggt ctggtttgtc ggatttgccc
     4261 ataccgatca ggtctggagc aatgcagcga tgggtcggtg caacatgcgg gatgatgttg
     4321 cgccacacgt aggaggaggt cgggttaccg tgcaggaaca gcacaggggt gccatcgcgc
     4381 ggaccaacat cgacgtagtg catgcgctcg cccaggactt ccacataatg ggggtcgaat
     4441 ggaaagccag taccgatttc tgccatatgg ctaccacgag gcaccaggcc gctgctgtga
     4501 tgatgatgat gatggctgct gcccatggtt gtggccatat tatcatcgtg tttttcaaag
     4561 gaaaaccacg tccccgtggt tcggggggcc tagacgtttt tttaacctcg actaaacaca
     4621 tgtaaagcat gtgtaccgag gccccagatc agatcccata caatggggta ccttctgggc
     4681 atccttcagc cccttgttga atacgcttga ggagagccat ttgactcttt ccacaactat
     4741 ccaactcaca acgtggcact ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg
     4801 cttttggccg cagaggcacc tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc
     4861 tacagacgtt gtttgtcttc aagaagcttc cagaggaact gcttccttca cgacattcaa
     4921 cagaccttgc attcctttgg cgagagggga aagaccccta ggaatgctcg tcaagaagac
     4981 agggccaggt ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataacat
     5041 atagacaaac gcacaccggc cttattccaa gcggcttcgg ccagtaacgt tagggggggg
     5101 ggagggagag gggcggatcc cgggcccgcg gtaccgtcga ctgcagaatt ggccgctaca
     5161 ggaacaggtg gtggcggccc tcggcgcgct cgtactgctc cacgatggtg tagtcctcgt
     5221 tgtgggaggt gatgtccagc ttggagtcca cgtagtagta gccgggcagc tgcacgggct
     5281 tcttggccat gtagatggac ttgaactcca ccaggtagtg gccgccgtcc ttcagcttca
     5341 gggccttgtg gatctcgccc ttcagcacgc cgtcgcgggg gtacaggcgc tcggtggagg
     5401 cctcccagcc catagtcttc ttctgcatta cggggccgtc ggaggggaag ttcacgccga
     5461 tgaacttcac cttgtagatg aaggagccgt cctgcaggga ggagtcctgg gtcacggtca
     5521 ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
     5581 gcttcttgta gtcggggatg tcggcggggt gcttcacgta caccttggag ccgtactgga
     5641 actgggggga caggatgtcc caggcgaagg gcagggggcc gcccttggtc accttcagct
     5701 tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt
     5761 ggccgttcac ggagccctcc atgcgcacct tgaagcgcat gaactccttg atgacgtcct
     5821 cggaggaggc catggtggat ccacggttca ctaaaccagc tctgcttata tagacctccc
     5881 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     5941 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     6001 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     6061 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     6121 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     6181 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     6241 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     6301 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     6361 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     6421 aactaagatc tggtaccttg aattcatgct tctcctccct ttagtgaggg taattctctc
     6481 tctctcccta tagtgagtcg tattaattcc ttctcttcta tagtgtcacc taaatcgttg
     6541 caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6601 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6661 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6721 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6781 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6841 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6901 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6961 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7021 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7081 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7141 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7201 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7261 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7321 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7381 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7441 ggaaaaagag ttggtagctc ttgatccggc aaaaaaacca ccgctggtag cggtggtttt
     7501 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7561 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7621 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7681 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7741 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7801 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7861 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7921 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7981 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8041 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8101 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8161 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8221 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8281 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8341 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8401 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8461 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8521 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8581 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8641 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8701 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     8761 acgagattgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     8821 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     8881 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     8941 atggctgatt atgatcctct agagtcggtg ggcctcgggg gcgggtgcgg ggtcggcggg
     9001 gccgccccgg gtggcttcgg tcggagccat ggggtcgtgc gctcctttcg gtcgggcgct
     9061 gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag gtgcgcggtc
     9121 cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt
     9181 tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc gcctccactc
     9241 cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag acgccgacgg
     9301 tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct tccatctgtt
     9361 gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc tcggcgaaca
     9421 ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg ccgtcgtccg
     9481 cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg
     9541 tgacccgctc gat
//
