LOCUS       sequence-174232-        8629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses his tagged human Keap1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Parvez S, Fu Y, Li J, Long MJ, Lin H, Lee DK, Hu GS, Aye Y
  TITLE     Substoichiometric Hydroxynonenylation of a Single Protein 
            Recapitulates Whole-Cell-Stimulated Antioxidant Response.
  JOURNAL   J Am Chem Soc. 2014 Dec 31.
  PUBMED    25544059
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2014 Dec 31."
COMMENT     Sequence Label: pMIR-Dsred-IRES-His Keap
FEATURES             Location/Qualifiers
     source          1..8629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     109..128
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(225..541)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      239..374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(581..603)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     703..722
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     930..947
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     931..947
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3548..3565)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(3575..3592)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(3605..3613,3614..3616,3617..4191)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3778..3797)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4007..4024
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(4234..4911)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(4296..4319)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(4494..4513)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4711..4731
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4763..4781
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(4936..5139)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4965..4985)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5140..5443
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(5564..5583)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5565..5583)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5595..5613)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5596..5613)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5633..5649)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5633..5649)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5646..5668)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5657..5673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5681..5687,5688..5705,5706..5711))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5726..5747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5864..5881)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6035..6623)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6115..6134)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6794..7585,7586..7654))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7417..7436
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7655..7759)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7847..7928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     7872..7891
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(8158..8629,1..128))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8241..8261)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 atgtggcggt ccgggtcgac ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg
       61 agggtgcgta cggcccgggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac
      121 tcggtcatgg aaggtcgtct ccttgtgagg ggtcaggggc gtgggtcagg ggatggtggc
      181 ggcaccggtc gtggcggccg acctgcaggc atgcaagcta gctttttgca aaagcctagg
      241 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
      301 tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga
      361 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
      421 atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
      481 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
      541 caatctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
      601 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
      661 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
      721 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
      781 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      841 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      901 taacgccagg gttttcccag acacgacgtt gtaaaacgac ggccagtgcc aagctagcgg
      961 ccgcatacaa aaaaccaaca cacagatcca atgaaaataa aagatccttt attaagctta
     1021 gctgaaaact tctggctcac atagcttctg tcaccaccaa tgagatgcca catgcgctga
     1081 ctacttgggt cacttttaat ttttatgttg ctgattgtat gtgtgcatct cctgtcttgt
     1141 aagcattaaa caaatgaaga catatgcaga gctgtgaaat tcactctgca ctgaatagac
     1201 aaaaagggtt acatagcata acaatgagca gttggtatcc acgtggttct cagcattata
     1261 aactctgggg aagatgctac ttcgtaacct caaggaaata agtaagcaag gcagaaggaa
     1321 cacttaaaca agtattattc aacagacatg tttacactaa ataattaagg ttatatttag
     1381 ggtttaaact catccctgaa tatccttagt aacaacctgg aggaaaaact ggggtgaatg
     1441 agaacttaag ttttccaagg attatggttc aaatcctctg aaatctataa caggttaaaa
     1501 aaatgagaac tcaactttct agagcaataa ctcttctcga gggcactagc acaaggcggt
     1561 agtgattttc acttttaata tattacatat aatactacaa aaagactgaa gttttaagga
     1621 aaatccatta ttattaaaag tttagtactc ttgagtctgg actttctgat caacaggtac
     1681 agttctgctg gtcaatctgc ttccggcagg gctccatggt gacagccacg cccaccccac
     1741 tccggcccga tgtcattcgg gtcacctcgc tccaggtgtc tgtatctggg tcgtaacact
     1801 ccacactgtc caggaacgtg tgaccatcat agcctccaag gacgtagatt ctcccctggt
     1861 ggacagtgat ccccagggca cttcgccggt gcttcatggg ggctacgaaa gtccacgtct
     1921 ctgtttccac atcgtagcgc tccacgctgt tcagctggtc ctgaccatca tagcccccag
     1981 cagcatagat acagttgtgc aggacgcaga cgcctgcccc gcttcggatg gtgttcattg
     2041 ctgtgatcat tcgccactcg ttcctctctg ggtagtaaca ctcagctgaa ttaaggcggt
     2101 ttgtcccgtc aaagcccccc acggcataaa ggagacgatt gaggacagcc acgcccaccc
     2161 cgatccttcg tgtcagcatt ggggccacca agtgccactc atcccgctct ggctcatacc
     2221 tctccacact gttgtggtgg atgcagccgt gggagccgcc gacggcatag atgtggccat
     2281 cgatgacccc caccccgatg cggttacggg gcacgctcat gggggcgcag ggcgaccact
     2341 gattggtcat ggggttgtaa cagtccaggg cgctggagtc ggtgttgccg tcgggcgagt
     2401 tgttcctgcc gcccacggcg tacaacagcc cgcccaccac gcagccggcc aggccgctcc
     2461 gcggcacctg caggtccgcc aaccggagcc aggtgccgtc actggggttg taagcctcca
     2521 ggtagctgag cgactgtcgg aagtagccgc ccgcggtgta gatcaggcgg cccaccttgg
     2581 gcgcccggca gggcatcacc tgcgtgggct tgtgcagggt gagctcctcg aagatcttga
     2641 ccaggtagtc cttgcagcgg gagtcggact gcaggatctc gcacttctgc agctgcatct
     2701 gcaggaagtt cggcgtcaac gagtggcagc gcacggcccg cagcagcgcc tggacgtaga
     2761 accgtcgctg ttcgcagtcg tacttgaccc agttgatgca ggcgtggaag acctcggact
     2821 cgcagcgcac gttcaggtcg tcccggctga tgagggtcac cagttggcag tgggacaggt
     2881 tgaagaactc ctcttgcttg gccacctccc caaaatgcat gtagatgtac tcccgggcac
     2941 gctggtgcaa ctccacacag ccaatctgct cagcgaagtt ggcgatgccg atggcattgc
     3001 tggggtccag ctgctgcacc aggaagtcac tgcaggcacg gacaacgctg tcgatctggt
     3061 acatgacagc accgttcatg acgtggagga cacacttctc gcccatggag atggaggccg
     3121 tgtaggcgaa ttcaatgagg cgctccatga ccttggggtg gataccctca atggacacca
     3181 cctccatgcc ctgctcccgc agcccgttgg tgaacatggc cttgaagaca gggctggatg
     3241 aggccagcac caccttgtgg gccatgaact gggcggccgg tgcatcctgg tacttgacct
     3301 gcagtgtgac gtcacacagc tgctggctga gccgcagctc gttcatgatg ccaaaggcct
     3361 gcttggtatg atcctccagg gtgtagctga aggtgcggtt gccatgctgg gagggcgtca
     3421 cctccgcctt gcactcagtg gaggcgtaca tcaccgcgtc ccctgccccc tcagggcact
     3481 gtgactgcag gggcaggaat cggcagcagg ccccagcccc gctaggcctg ggatctggct
     3541 gcatatggct accacgaggc accaggccgc tgctgtgatg atgatgatga tggctgctgc
     3601 ccatggttgt ggccatatta tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc
     3661 ggggggccta gacgtttttt taacctcgac taaacacatg taaagcatgt gtaccgaggc
     3721 cccagatcag atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat
     3781 acgcttgagg agagccattt gactctttcc acaactatcc aactcacaac gtggcactgg
     3841 ggttgtgccg cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg
     3901 tcgccaggtg gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa
     3961 gaagcttcca gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg
     4021 agaggggaaa gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc
     4081 acattgccaa aagacggcaa tatggtggaa aataacatat agacaaacgc acaccggcct
     4141 tattccaagc ggcttcggcc agtaacgtta gggggggggg agggagaggg gcggatcccg
     4201 ggcccgcggt accgtcgact gcagaattgg ccgctacagg aacaggtggt ggcggccctc
     4261 ggcgcgctcg tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
     4321 ggagtccacg tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt
     4381 gaactccacc aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt
     4441 cagcacgccg tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tagtcttctt
     4501 ctgcattacg gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa
     4561 ggagccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
     4621 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc
     4681 ggcggggtgc ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca
     4741 ggcgaagggc agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta
     4801 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
     4861 gcgcaccttg aagcgcatga actccttgat gacgtcctcg gaggaggcca tggtggatcc
     4921 acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     4981 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     5041 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     5101 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     5161 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     5221 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     5281 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     5341 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     5401 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     5461 gctatgaact aatgaccccg taattgatta ctattaataa ctaagatctg gtaccttgaa
     5521 ttcatgcttc tcctcccttt agtgagggta attctctctc tctccctata gtgagtcgta
     5581 ttaattcctt ctcttctata gtgtcaccta aatcgttgca attcgtaatc atgtcatagc
     5641 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5701 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5761 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     5821 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     5881 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5941 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6001 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6061 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6121 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6181 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6241 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6301 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6361 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6421 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6481 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6541 gatccggcaa aaaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6601 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6661 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6721 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6781 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6841 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6901 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6961 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7021 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7081 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7141 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7201 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7261 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7321 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7381 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7441 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7501 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7561 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7621 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7681 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7741 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     7801 ttattatcat gacattaacc tataaaaata ggcgtatcac gagattgcag tgaaaaaaat
     7861 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     7921 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     7981 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag
     8041 agtcggtggg cctcgggggc gggtgcgggg tcggcggggc cgccccgggt ggcttcggtc
     8101 ggagccatgg ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg gcgggcgtca
     8161 ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc
     8221 ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag
     8281 gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc
     8341 cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc acgcgggctc
     8401 cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc
     8461 gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc
     8521 cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc
     8581 gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcg
//
