LOCUS       sequence-174239-        8882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  research of alternative isoforms of the protein dysferlin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8882)
  TITLE     Jain Foundation plasmids
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUC57 DYSF, alt exon 1, 40a
FEATURES             Location/Qualifiers
     source          1..8882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..36
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     28..45
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     29..45
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             736..747
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6356..6372)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6356..6372)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6369..6391)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6380..6396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6404..6410,6411..6428,6429..6434))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6449..6470
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6587..6604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6758..7346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6838..6857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7517..8332)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7517..8332)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8240..8259
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8333..8437)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8505..8523
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8561..8583)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8683..8702
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatg
       61 ctgagggtct tcatcctcta tgccgagaac gtccacacac ccgacaccga catcagcgat
      121 gcctactgct ccgcggtgtt tgcaggggtg aagaagagaa ccaaagtcat caagaacagc
      181 gtgaaccctg tatggaatga gggatttgaa tgggacctca agggcatccc cctggaccag
      241 ggctctgagc ttcatgtggt ggtcaaagac catgagacga tggggaggaa caggttcctg
      301 ggggaagcca aggtcccact ccgagaggtc ctcgccaccc ctagtctgtc cgccagcttc
      361 aatgcccccc tgctggacac caagaagcag cccacagggg cctcgctggt cctgcaggtg
      421 tcctacacac cgctgcctgg agctgtgccc ctgttcccgc cccctactcc tctggagccc
      481 tccccgactc tgcctgacct ggatgtagtg gcagacacag gaggagagga agacacagag
      541 gaccagggac tcactggaga tgaggcggag ccattcctgg atcaaagcgg aggcccgggg
      601 gctcccacca ccccaaggaa actaccttca cgtcctccgc cccactaccc cgggatcaaa
      661 agaaagcgaa gtgcgcctac atctagaaag ctgctgtcag acaaaccgca ggatttccag
      721 atcagggtcc aggtgatcga ggggcgccag ctgccggggg tgaacatcaa gcctgtggtc
      781 aaggttaccg ctgcagggca gaccaagcgg acgcggatcc acaagggaaa cagcccactc
      841 ttcaatgaga ctcttttctt caacttgttt gactctcctg gggagctgtt tgatgagccc
      901 atctttatca cggtggtaga ctctcgttct ctcaggacag atgctctcct cggggagttc
      961 cggatggacg tgggcaccat ttacagagag ccccggcacg cctatctcag gaagtggctg
     1021 ctgctctcag accctgatga cttctctgct ggggccagag gctacctgaa aacaagcctt
     1081 tgtgtgctgg ggcctgggga cgaagcgcct ctggagagaa aagacccctc tgaagacaag
     1141 gaggacattg aaagcaacct gctccggccc acaggcgtag ccctgcgagg agcccacttc
     1201 tgcctgaagg tcttccgggc cgaggacttg ccgcagatgg acgatgccgt gatggacaac
     1261 gtgaaacaga tctttggctt cgagagtaac aagaagaact tggtggaccc ctttgtggag
     1321 gtcagctttg cggggaaaat gctgtgcagc aagatcttgg agaagacggc caaccctcag
     1381 tggaaccaga acatcacact gcctgccatg tttccctcca tgtgcgaaaa aatgaggatt
     1441 cgtatcatag actgggaccg cctgactcac aatgacatcg tggctaccac ctacctgagt
     1501 atgtcgaaaa tctctgcccc tggaggagaa atagaagtgg atgactacct gggcttcctc
     1561 cccacttttg ggccctgcta catcaacctc tatggcagtc ccagagagtt cacaggcttc
     1621 ccagacccct acacagagct caacacaggc aagggggaag gtgtggctta tcgtggccgg
     1681 cttctgctct ccctggagac caagctggtg gagcacagtg aacagaaggt ggaggacctt
     1741 cctgcggatg acatcctccg ggtggagaag taccttagga ggcgcaagta ctccctgttt
     1801 gcggccttct actcagccac catgctgcag gatgtggatg acgccatcca gtttgaggtc
     1861 agcatcggga actacgggaa caagttcgac atgacctgcc tgccgctggc ctccaccact
     1921 cagtacagcc gtgcagtctt tgacgggtgc cactactact acctaccctg gggtaacgtg
     1981 aaacccgtgg tggtgctgtc atcctactgg gaggacatca gccatagaat cgagactcag
     2041 aaccagctgc ttgggattgc tgaccggctg gaagctggcc tggagcaggt ccacctggcc
     2101 ctgaaggcgc agtgctccac ggaggacgtg gactcgctgg tggctcagct gacggatgag
     2161 ctcatcgcag gctgcagcca gcctctgggt gacatccatg agacaccctc tgccacccac
     2221 ctggaccagt acctgtacca gctgcgcacc catcacctga gccaaatcac tgaggctgcc
     2281 ctggccctga agctcggcca cagtgagctc cctgcagctc tggagcaggc ggaggactgg
     2341 ctcctgcgtc tgcgtgccct ggcagaggag ccccagaaca gcctgccgga catcgtcatc
     2401 tggatgctgc agggagacaa gcgtgtggca taccagcggg tgcctgccca ccaagtcctc
     2461 ttctcccggc ggggtgccaa ctactgtggc aagaattgtg ggaagctaca gacaatcttt
     2521 ctgaaatatc cgatggagaa ggtgcctggc gcccggatgc cagtgcagat acgggtcaag
     2581 ctgtggtttg ggctctctgt ggatgagaag gagttcaacc agtttgctga ggggaagctg
     2641 tctgtctttg ctgaaaccta tgagaacgag actaagttgg cccttgttgg gaactggggc
     2701 acaacgggcc tcacctaccc caagttttct gacgtcacgg gcaagatcaa gctacccaag
     2761 gacagcttcc gcccctcggc cggctggacc tgggctggag attggttcgt gtgcccggag
     2821 aagactctgc tccatgacat ggacgccggt cacctgagct tcgtggaaga ggtgtttgag
     2881 aaccagaccc ggcttcccgg aggccagtgg atctacatga gtgacaacta caccgatgtg
     2941 aacggggaga aggtgcttcc caaggatgac attgagtgcc cactgggctg gaagtgggaa
     3001 gatgaggaat ggtccacaga cctcaaccgg gctgtcgatg agcaaggctg ggagtatagc
     3061 atcaccatcc ccccggagcg gaagccgaag cactgggtcc ctgctgagaa gatgtactac
     3121 acacaccgac ggcggcgctg ggtgcgcctg cgcaggaggg atctcagcca aatggaagca
     3181 ctgaaaaggc acaggcaggc ggaggcggag ggcgagggct gggagtacgc ctctcttttt
     3241 ggctggaagt tccacctcga gtaccgcaag acagatgcct tccgccgccg ccgctggcgc
     3301 cgtcgcatgg agccactgga gaagacgggg cctgcagctg tgtttgccct tgagggggcc
     3361 ctgggcggcg tgatggatga caagagtgaa gattccatgt ccgtctccac cttgagcttc
     3421 ggtgtgaaca gacccacgat ttcctgcata ttcgactatg ggaaccgcta ccatctacgc
     3481 tgctacatgt accaggcccg ggacctggct gcgatggaca aggactcttt ttctgatccc
     3541 tatgccatcg tctccttcct gcaccagagc cagaagacgg tggtggtgaa gaacaccctt
     3601 aaccccacct gggaccagac gctcatcttc tacgagatcg agatctttgg cgagccggcc
     3661 acagttgctg agcaaccgcc cagcattgtg gtggagctgt acgaccatga cacttatggt
     3721 gcagacgagt ttatgggtcg ctgcatctgt caaccgagtc tggaacggat gccacggctg
     3781 gcctggttcc cactgacgag gggcagccag ccgtcggggg agctgctggc ctcttttgag
     3841 ctcatccaga gagagaagcc ggccatccac catattcctg gttttgaggt gcaggagaca
     3901 tcaaggatcc tggatgagtc tgaggacaca gacctgccct acccaccacc ccagagggag
     3961 gccaacatct acatggttcc tcagaacatc aagccagcgc tccagcgtac cgccatcgag
     4021 atcctggcat ggggcctgcg gaacatgaag agttaccagc tggccaacat ctcctccccc
     4081 agcctcgtgg tagagtgtgg gggccagacg gtgcagtcct gtgtcatcag gaacctccgg
     4141 aagaacccca actttgacat ctgcaccctc ttcatggaag tgatgctgcc cagggaggag
     4201 ctctactgcc cccccatcac cgtcaaggtc atcgataacc gccagtttgg ccgccggcct
     4261 gtggtgggcc agtgtaccat ccgctccctg gagagcttcc tgtgtgaccc ctactcggcg
     4321 gagagtccat ccccacaggg tggcccagac gatgtgagcc tactcagtcc tggggaagac
     4381 gtgctcatcg acattgatga caaggagccc ctcatcccca tccagcttgc agacggtctg
     4441 tcgagcttgg cccccactaa cacggcttct cctccatcca gtcctcatga ggaagagttc
     4501 atcgattggt ggagcaaatt ctttgcctcc ataggggaga gggaaaagtg cggctcctac
     4561 ctggagaagg attttgacac cctgaaggtc tatgacacac agctggagaa tgtggaggcc
     4621 tttgagggcc tgtctgactt ttgtaacacc ttcaagctgt accggggcaa gacgcaggag
     4681 gagacagaag atccatctgt gattggtgaa tttaagggcc tcttcaaaat ttatcccctc
     4741 ccagaagacc cagccatccc catgccccca agacagttcc accagctggc cgcccaggga
     4801 ccccaggagt gcttggtccg tatctacatt gtccgagcat ttggcctgca gcccaaggac
     4861 cccaatggaa agtgtgatcc ttacatcaag atctccatag ggaagaaatc agtgagtgac
     4921 caggataact acatcccctg cacgctggag cccgtatttg gaaagatgtt cgagctgacc
     4981 tgcactctgc ctctggagaa ggacctaaag atcactctct atgactatga cctcctctcc
     5041 aaggacgaaa agatcggtga gacggtcgtc gacctggaga acaggctgct gtccaagttt
     5101 ggggctcgct gtggactccc acagacctac tgtgtctctg gaccgaacca gtggcgggac
     5161 cagctccgcc cctcccagct cctccacctc ttctgccagc agcatagagt caaggcacct
     5221 gtgtaccgga cagaccgtgt aatgtttcag gataaagaat attccattga agagatagag
     5281 gctggcagga tcccaaaccc acacctgggc ccagtggagg agcgtctggc tctgcatgtg
     5341 cttcagcagc agggcctggt cccggagcac gtggagtcac ggcccctcta cagccccctg
     5401 cagccagaca tcgagcaggg gaagctgcag atgtgggtcg acctatttcc gaaggccctg
     5461 gggcggcctg gacctccctt caacatcacc ccacggagag ccagaaggtt tttcctgcgt
     5521 tgtattatct ggaataccag agatgtgatc ctggatgacc tgagcctcac gggggagaag
     5581 atgagcgaca tttatgtgaa aggttggatg attggctttg aagaacacaa gcaaaagaca
     5641 gacgtgcatt atcgttccct gggaggtgaa ggcaacttca actggaggtt cattttcccc
     5701 ttcgactacc tgccagctga gcaagtctgt accattgcca agaaggatgc cttctggagg
     5761 ctggacaaga ctgagagcaa aatcccagca cgagtggtgt tccagatctg ggacaatgac
     5821 aagttctcct ttgatgattt tctgggctcc ctgcagctcg atctcaaccg catgcccaag
     5881 ccagccaaga cagccaagaa gtgctccttg gaccagctgg atgatgcttt ccacccagaa
     5941 tggtttgtgt ccctttttga gcagaaaaca gtgaagggct ggtggccctg tgtagcagaa
     6001 gagggtgaga agaaaatact ggcgggcaag ctggaaatga ccttggagat tgtagcagag
     6061 agtgagcatg aggagcggcc tgctggccag ggccgggatg agcccaacat gaaccctaag
     6121 cttgaggacc caaggcgccc cgacacctcc ttcctgtggt ttacctcccc atacaagacc
     6181 atgaagttca tcctgtggcg gcgtttccgg tgggccatca tcctcttcat catcctcttc
     6241 atcctgctgc tgttcctggc catcttcatc tacgccttcc cgaactatgc tgccatgaag
     6301 ctggtgaagc ccttcagctg aatcccaatg gcgcgccgag cttggctcga gcatggtcat
     6361 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6421 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6481 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6541 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6601 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6661 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6721 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6781 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6841 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6901 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6961 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7021 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7081 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7141 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7201 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7261 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7321 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7381 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7441 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7501 aaacttggtc tgacagttag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     7561 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     7621 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     7681 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     7741 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
     7801 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     7861 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     7921 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     7981 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccaggg atcgcagtgg
     8041 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     8101 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     8161 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     8221 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     8281 tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc atactcttcc
     8341 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8401 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8461 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     8521 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     8581 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     8641 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     8701 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     8761 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     8821 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     8881 ta
//
