LOCUS       sequence-174252-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA against TOM40..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Yano H, Baranov SV, Baranova OV, Kim J, Pan Y, Yablonska S, Carlisle
            DL, Ferrante RJ, Kim AH, Friedlander RM
  TITLE     Inhibition of mitochondrial protein import by mutant huntingtin.
  JOURNAL   Nat Neurosci. 2014 Jun;17(6):822-31. doi: 10.1038/nn.3721. Epub 2014
            May 18.
  PUBMED    24836077
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3721"; 
            journalName: "Nat Neurosci"; date: "2014-06"; volume: "17"; issue: 
            "6"; pages: "822-31"
COMMENT     Sequence Label: pBSU6-shTOM40.3
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..14
                     /estimated_length=13
     primer_bind     32..48
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        45..358
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     277..297
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(435..451)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(488..508)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(488..506)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(527..543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(527..543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(540..562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    551..567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(575..581,582..599,600..605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    620..641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(758..775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gnnnnnnnnn nnnnggcgat tgggnnnccg ctctagaact agtggatccg acgccgccat
       61 ctctaggccc gcgccggccc cctcgcacag acttgtggga gaagctcggc tactcccctg
      121 ccccggttaa tttgcatata atatttccta gtaactatag aggcttaatg tgcgataaaa
      181 gacagataat ctgttctttt taatactagc tacattttac atgataggct tggatttcta
      241 taagagatac aaatactaaa ttattatttt aaaaaacagc acaaaaggaa actcacccta
      301 actgtaaagt aattgtgtgt tttgagacta taaatatccc ttggagaaaa gccttgtttg
      361 gcactgtcat gtctctagca ttcaagctta gctagagaca tgacagtgcc tttttgaatt
      421 cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg agctccagct
      481 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
      541 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      601 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      661 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      721 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      781 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      841 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aangncagga
      901 accgtaaaaa ggccgcgttg ctggcgtttt tccatagnnt ccgcccccct gacgagcatc
      961 acaaaaatcg acgctcnagt canangtggc ga
//
