LOCUS       sequence-174257-        8519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral vector encoding cre dependent expression of membrane 
            Tdtomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Lim L, Pakan JMP, Selten MM, Marques-Smith A, Llorca A, Bae SE, 
            Rochefort NL, Marin O
  TITLE     Optimization of interneuron function by direct coupling of cell 
            migration and axonal targeting.
  JOURNAL   Nat Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 
            10.1038/s41593-018-0162-9.
  PUBMED    29915195
REFERENCE   2  (bases 1 to 8519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 
            10.1038/s41593-018-0162-9."
COMMENT     Sequence Label: RV-Cag-Dio-mTdt
FEATURES             Location/Qualifiers
     source          1..8519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        266..645
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        646..849
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     800..820
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             850..1025
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    1088..1287
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1488..1904
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1834..1856
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1872..1888
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1918..2297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2299..2574
                     /label=chicken beta-actin promoter
     primer_bind     3494..3516
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3578..3597
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(3640..3673)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3724..3757)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3766..5196)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    5268..5301
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5352..5385
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5395..5983
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5448..5468)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5866..5877)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6854..6871)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7025..7613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7105..7124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7773..8519,1..45,46..114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8396..8415
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
       61 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      121 cctttttcaa tgatccggcc attagccata ttattcattg gttatatagc ataaatcaat
      181 attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
      241 tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca
      301 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      361 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      421 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      481 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      541 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      601 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      661 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      721 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      781 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      841 agcagagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac
      901 tgagtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat ccgaatcgtg
      961 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccacga cgggggtctt
     1021 tcatttgggg gctcgtccgg gatttggaga cccctgccca gggaccaccg acccaccacc
     1081 gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg tctatgtttg
     1141 atgttatgcg cctgcgtctg tactagttag ctaactagct ctgtatctgg cggacccgtg
     1201 gtggaactga cgagttctga acacccggcc gcaaccctgg gagacgtccc agggactttg
     1261 ggggccgttt ttgtggcccg acctgaggaa gggagtcgat gtggaatccg accccgtcag
     1321 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt
     1381 tgctttcggt ttggaaccga agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc
     1441 tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaattagggc cagactgtta
     1501 ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc
     1561 agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct
     1621 ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga
     1681 tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct
     1741 gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc
     1801 cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc
     1861 ctcgatcctc cctttatcca gccctcactc cttctctagg cgccggaatt cgatgtcgac
     1921 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     1981 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2041 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2101 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2161 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2221 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2281 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     2341 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     2401 gggcgggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2461 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2521 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2581 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     2641 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     2701 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
     2761 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
     2821 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     2881 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     2941 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     3001 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     3061 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     3121 gggctcgccg tgccgggcag gtgggggggg gcggggccgc ctcgggccgg ggagggctcg
     3181 ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc
     3241 cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt
     3301 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg
     3361 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
     3421 ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga
     3481 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     3541 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     3601 tctcatcatt ttggcaaaga attcgctagc ggatccggaa taacttcgta taggatactt
     3661 tatacgaagt tatgcagaat ggtagctgga ttgtagctgc tattagcaat atgaaacctc
     3721 ttaataactt cgtatagcat acattatacg aagttattta attaattact tgtacagctc
     3781 gtccatgccg tacaggaaca ggtggtggcg gccctcggag cgctcgtact gttccacgat
     3841 ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggtg tccacgtagt agtagccggg
     3901 cagttgcacg ggcttcttgg ccatgtagat ggtcttgaac tccaccaggt agtggccgcc
     3961 gtccttcagc ttcagggcct ggtggatctc gcccttcagc acgccgtcgc gggggtacag
     4021 gcgctcggtg gaggcctccc agcccatggt cttcttctgc attacggggc cgtcgggggg
     4081 gaagttggtg ccgcgcatct tcaccttgta gatcagcgtg ccgtcctgca gggaggagtc
     4141 ctgggtcacg gtcaccagac cgccgtcctc gaagttcatc acgcgctccc acttgaagcc
     4201 ctcggggaag gacagcttct tgtaatcggg gatgtcggcg gggtgcttca cgtacgcctt
     4261 ggagccgtac atgaactggg gggacaggat gtcccaggcg aagggcaggg ggccgccctt
     4321 ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc
     4381 gatctcgaac tcgtggccgt tcatggagcc ctccatgcgc accttgaagc gcatgaactc
     4441 tttgatgacg gccatgttgt tgtcctcgga ggaggcggtg ccggagctgc cgctgccggt
     4501 gctgccggtg ccatgcccca ggaacaggtg gtggcggccc tcggagcgct cgtactgttc
     4561 cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc ttggtgtcca cgtagtagta
     4621 gccgggcagt tgcacgggct tcttggccat gtagatggtc ttgaactcca ccaggtagtg
     4681 gccgccgtcc ttcagcttca gggcctggtg gatctcgccc ttcagcacgc cgtcgcgggg
     4741 gtacaggcgc tcggtggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc
     4801 gggggggaag ttggtgccgc gcatcttcac cttgtagatc agcgtgccgt cctgcaggga
     4861 ggagtcctgg gtcacggtca ccagaccgcc gtcctcgaag ttcatcacgc gctcccactt
     4921 gaagccctcg gggaaggaca gcttcttgta atcggggatg tcggcggggt gcttcacgta
     4981 cgccttggag ccgtacatga actgggggga caggatgtcc caggcgaagg gcagggggcc
     5041 gcccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc
     5101 gccctcgatc tcgaactcgt ggccgttcat ggagccctcc atgcgcacct tgaagcgcat
     5161 gaactctttg atgacctcct cgcccttgct caccatgatc ttttggtcct catcattctt
     5221 ttcaacctgt ttggttcttc tcatacagca cagcatggcg cgccgcgata acttcgtata
     5281 aagtatccta tacgaagtta tttgccttaa cccagaaatt atcactgtta ttctttagaa
     5341 tggtgcaaag aataacttcg tataatgtat gctatacgaa gttatgttta aaccaatcaa
     5401 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5461 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5521 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5581 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5641 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5701 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5761 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     5821 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     5881 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5941 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcga
     6001 gcttgttaac atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     6061 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     6121 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
     6181 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     6241 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     6301 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     6361 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     6421 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     6481 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     6541 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     6601 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcrcat ccgacttgtg
     6661 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     6721 ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat
     6781 ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6841 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6901 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6961 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7021 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7441 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7681 tggtcatgag attatcaaaa aggatcttca cctagatcct tttgcggccg caaatcaatc
     7741 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7801 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7861 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7921 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7981 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8041 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8101 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8161 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8221 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8281 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8341 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8401 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8461 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcacc
//
