LOCUS       sequence-174260-        5802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5802)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-Actin-7
FEATURES             Location/Qualifiers
     source          1..5802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..693
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     polyA_signal    1978..2099
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2015..2034)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2069..2088
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2106..2561)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2243..2264)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2455..2474
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2588..2692
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2694..3051
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2694..2885
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2717..2737)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2902..3037
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3086..3880
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3086..3880
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3140..3159)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3750..3769
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4068..4087)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4112..4159
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4488..5076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4977..4996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5251..5554
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5555..5758
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5709..5729
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgagcgagc tgatcaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
       61 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      121 atgaagatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
      181 agcttcatgt acggcagcaa agccttcatc aaccacaccc agggcatccc cgacttcttt
      241 aagcagtcct tccctgaggg cttcacatgg gagagaatca ccacatacga agacgggggc
      301 gtgctgaccg ctacccagga caccagcttc cagaacggct gcatcatcta caacgtcaag
      361 atcaacgggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac acgcggctgg
      421 gaggccaaca ccgagatgct gtaccccgct gacggcggcc tgagaggcca cagccagatg
      481 gccctgaagc tcgtgggcgg gggctacctg cactgctcct tcaagaccac atacagatcc
      541 aagaaacccg ctaagaacct caagatgccc ggcttccact tcgtggacca cagactggaa
      601 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgagatggc tgtggccaag
      661 tactgcgacc tccctagcaa actggggcac agagatgaat ccggactcag atctcgagct
      721 atggatgatg atatcgccgc gctcgtcgtc gacaacggct ccggcatgtg caaggccggc
      781 ttcgcgggcg acgatgcccc ccgggccgtc ttcccctcca tcgtggggcg ccccaggcac
      841 cagggcgtga tggtgggcat gggtcagaag gattcctatg tgggcgacga ggcccagagc
      901 aagagaggca tcctcaccct gaagtacccc atcgagcacg gcatcgtcac caactgggac
      961 gacatggaga aaatctggca ccacaccttc tacaatgagc tgcgtgtggc tcccgaggag
     1021 caccccgtgc tgctgaccga ggcccccctg aaccccaagg ccaaccgcga gaagatgacc
     1081 cagatcatgt ttgagacctt caacacccca gccatgtacg ttgctatcca ggctgtgcta
     1141 tccctgtacg cctctggccg taccactggc atcgtgatgg actccggtga cggggtcacc
     1201 cacactgtgc ccatctacga ggggtatgcc ctcccccatg ccatcctgcg tctggacctg
     1261 gctggccggg acctgactga ctacctcatg aagatcctca ccgagcgcgg ctacagcttc
     1321 accaccacgg ccgagcggga aatcgtgcgt gacattaagg agaagctgtg ctacgtcgcc
     1381 ctggacttcg agcaagagat ggccacggct gcttccagct cctccctgga gaagagctac
     1441 gagctgcctg acggccaggt catcaccatt ggcaatgagc ggttccgctg ccctgaggca
     1501 ctcttccagc cttccttcct gggcatggag tcctgtggca tccacgaaac taccttcaac
     1561 tccatcatga agtgtgacgt ggacatccgc aaagacctgt acgccaacac agtgctgtct
     1621 ggcggcacca ccatgtaccc tggcattgcc gacaggatgc agaaggagat cactgccctg
     1681 gcacccagca caatgaagat caagatcatt gctcctcctg agcgcaagta ctccgtgtgg
     1741 atcggcggct ccatcctggc ctcgctgtcc accttccagc agatgtggat cagcaagcag
     1801 gagtatgacg agtccggccc ctccatcgtc caccgcaaat gcttctaggg atccaccgga
     1861 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     1921 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     1981 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2041 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     2101 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2161 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2221 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2281 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     2341 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     2401 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     2461 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     2521 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     2581 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2641 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     2701 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     2761 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     2821 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     2881 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     2941 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     3001 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     3061 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     3121 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3181 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3241 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     3301 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     3361 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     3421 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     3481 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     3541 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     3601 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     3661 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     3721 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     3781 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     3841 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     3901 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     3961 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     4021 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     4081 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     4141 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     4201 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     4261 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     4321 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4381 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4441 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4501 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4561 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4621 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4681 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4741 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4801 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4861 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4921 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4981 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5041 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5101 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5161 tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
     5221 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     5281 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     5341 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     5401 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     5461 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     5521 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     5581 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     5641 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     5701 cccattgacg caaatgggcg gtaggcgtgt acggtggaag gtctatataa gcagagctgg
     5761 tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca cc
//
