LOCUS       sequence-174286-        9364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9364)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 9364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: pLX302-MKP5-V5 puro
FEATURES             Location/Qualifiers
     source          1..9364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      44..632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     533..552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     786..803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    920..941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(956..961,962..979,980..986)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    994..1010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     999..1021
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1018..1034
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1018..1034
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1053..1073
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1055..1073
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1101..1327
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1328..1508
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1555..1680
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2173..2406
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2591..2635
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2810..3310
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3229..3247
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3332..3931
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3332..3351)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3828..3848
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3973..4090
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4207..4510
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4511..4714
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4665..4685
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4737..4761
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6208..6232)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6234..6275
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6317..6905
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6370..6390)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6788..6799)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6977..7210
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7288..7409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7325..7344)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7379..7398
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7449..7584
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7604..7623)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7605..7623)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7633..7650)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7633..7649)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7642..7664)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7791..8246
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7878..7897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8088..8109
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8272..8376
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8377..8445,8446..9237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8595..8614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
       61 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      121 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      181 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      241 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      301 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      361 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      421 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      481 atccggtaag cggcagggtc ggaacaggag agcgcactag ggagcttcca gggggaaacg
      541 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      601 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      661 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      721 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
      781 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
      841 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      901 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      961 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     1021 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     1081 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     1141 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     1201 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     1261 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     1321 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     1381 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     1441 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     1501 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     1561 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     1621 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     1681 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     1741 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1801 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1861 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1921 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1981 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     2041 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     2101 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     2161 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     2221 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     2281 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     2341 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     2401 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     2461 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     2521 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2581 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     2641 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     2701 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     2761 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccct tgcgcctttt
     2821 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     2881 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     2941 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     3001 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     3061 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     3121 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     3181 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     3241 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     3301 ctctccccag ggggtaccac cggagcttac catgaccgag tacaagccca cggtgcgcct
     3361 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     3421 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     3481 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     3541 cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     3601 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     3661 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     3721 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     3781 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     3841 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     3901 ctggtgcatg acccgcaagc ccggtgcctg agaattctag atcttgagac aaatggcagt
     3961 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     4021 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     4081 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg
     4141 ggcccggggt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     4201 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     4261 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     4321 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     4381 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     4441 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     4501 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     4561 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     4621 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     4681 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactgtcg ggatcaacaa
     4741 gtttgtacaa aaaagttggc atgcctccgt ctcctttaga cgacagggta gtagtggcac
     4801 tatctaggcc cgtccgacct caggatctca acctttgttt agactctagt taccttggct
     4861 ctgccaaccc aggcagtaac agccaccctc ctgtcatcgc caccaccgtt gtgtccctca
     4921 aggctgcgaa tctgacgtat atgccctcat ccagcggctc tgcccgctcg ctgaattgtg
     4981 gatgcagcag tgccagctgc tgcactgtgg caacctacga caaggacaat caggcccaaa
     5041 cccaagccat tgccgctggc accaccacca ctgccatcgg aacctctacc acctgccctg
     5101 ctaaccagat ggtcaacaat aatgagaata caggctctct aagtccatca agtggggtgg
     5161 gcagccctgt gtcagggacc cccaagcagc tagccagcat caaaataatc taccccaatg
     5221 acttggcaaa gaagatgacc aaatgcagca agagtcacct gccgagtcag ggccctgtca
     5281 tcattgactg caggcccttc atggagtaca acaagagtca catccaagga gctgtccaca
     5341 ttaactgtgc cgataagatc agccggcgga gactgcagca gggcaagatc actgtcctag
     5401 acttgatttc ctgtagggaa ggcaaggact ctttcaagag gatcttttcc aaagaaatta
     5461 tagtttatga tgagaatacc aatgaaccaa gccgagtgat gccctcccag ccacttcaca
     5521 tagtcctcga gtccctgaag agagaaggca aagaacctct ggtgttgaaa ggtggactta
     5581 gtagttttaa gcagaaccat gaaaacctct gtgacaactc cctccagctc caagagtgcc
     5641 gggaggtggg gggcggcgca tccgcggcct cgagcttgct acctcagccc atccccacca
     5701 cccctgacat cgagaacgct gagctcaccc ccatcttgcc cttcctgttc cttggcaatg
     5761 agcaggatgc tcaggacctg gacaccatgc agcggctgaa catcggctac gtcatcaacg
     5821 tcaccactca tcttcccctc taccactatg agaaaggcct gttcaactac aagcggctgc
     5881 cagccactga cagcaacaag cagaacctgc ggcagtactt tgaagaggct tttgagttca
     5941 ttgaggaagc tcaccagtgt gggaaggggc ttctcatcca ctgccaggct ggggtgtccc
     6001 gctccgccac catcgtcatc gcttacttga tgaagcacac tcggatgacc atgactgatg
     6061 cttataaatt tgtcaaaggc aaacgaccaa ttatctcccc aaaccttaac ttcatggggc
     6121 agttgctaga gttcgaggaa gacctaaaca acggtgtgac accgagaatc cttacaccaa
     6181 agctgatggg cgtggagacg gttgtgtgcc caactttctt gtacaaagtg gttggtaagc
     6241 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
     6301 ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6361 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6421 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6481 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6541 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6601 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6661 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     6721 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6781 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6841 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     6901 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6961 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     7021 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7081 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7141 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7201 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
     7261 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7321 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7381 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     7441 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7501 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7561 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     7621 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     7681 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     7741 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     7801 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7861 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7921 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7981 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8041 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8101 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8161 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     8221 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     8281 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8341 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8401 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8461 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8521 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8581 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8641 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8701 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8761 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8821 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8881 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8941 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9001 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9061 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9121 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9181 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9241 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9301 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9361 tcgt
//
