LOCUS       sequence-174292-        1230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses syntaxin17 lacking N-terminal domain tagged with GFP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Uematsu M, Nishimura T, Sakamaki Y, Yamamoto H, Mizushima N
  TITLE     Accumulation of undegraded autophagosomes by expression of 
            dominant-negative STX17 (syntaxin 17) mutants.
  JOURNAL   Autophagy. 2017 Aug 3;13(8):1452-1464. doi: 
            10.1080/15548627.2017.1327940. Epub 2017 Jun 9.
  PUBMED    28598244
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2017.1327940"; journalName: "Autophagy"; date: 
            "2017-08-3- 3"; volume: "13"; issue: "8"; pages: "1452-1464"
COMMENT     Sequence Label: pMRXIP-GFP-STX17-Delta-NTD
FEATURES             Location/Qualifiers
     source          1..1230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt
      721 ggcggtggct cgggcggtgg tgggtcgggt ggcggcggat ctatcgatga attgaattca
      781 cgcgttcgcg agttaacgga tccgattcct caagatcaaa atgctgcaga atcgtgggaa
      841 accttagaag cggacttaat tgaacttagc caactggtca ctgacttctc tctcctagtg
      901 aattctcagc aggagaagat tgacagcatt gcagaccatg tcaacagtgc tgctgtgaat
      961 gttgaagagg gaaccaaaaa cttagggaag gctgcaaaat acaagctggc agctctgcct
     1021 gtggcaggtg cactcatcgg gggaatggta gggggtccta ttggcctcct tgcaggcttc
     1081 aaagtggcag gaattgcagc tgcacttggt ggtggggtgt tgggcttcac aggtggaaaa
     1141 ttgatacaaa gaaagaaaca gaaaatgatg gagaagctca cttccagctg tccagatctt
     1201 cccagccaaa ctgacaagaa atgcagttaa
//
