LOCUS       sequence-174295-        9903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5 tagged human MAPK phosphatase 7 (MKP7/DUSP16).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 9903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: pLX302-MKP7-V5 puro
FEATURES             Location/Qualifiers
     source          1..9903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     517..536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     770..787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    904..925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(940..945,946..963,964..970)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    978..994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     983..1005
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1002..1018
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1002..1018
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1037..1057
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1039..1057
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1085..1311
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1312..1492
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1539..1664
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2157..2390
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2575..2619
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2794..3294
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3213..3231
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3316..3915
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3316..3335)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3812..3832
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3957..4074
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4191..4494
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4495..4698
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4649..4669
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4721..4745
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6731..6755)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6757..6798
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6840..7428
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6893..6913)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7311..7322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7500..7733
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7811..7932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7848..7867)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7902..7921
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7972..8107
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8127..8146)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8128..8146)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8156..8173)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8156..8172)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8165..8187)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8314..8769
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8401..8420)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8611..8632
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8795..8899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8900..8968,8969..9760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9118..9137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
       61 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      121 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      181 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      241 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      301 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      361 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      421 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      481 ggtcggaaca ggagagcgca ctagggagct tccaggggga aacgcctggt atctttatag
      541 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      601 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      661 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      721 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      781 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      841 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      901 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      961 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     1021 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     1081 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     1141 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     1201 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     1261 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     1321 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     1381 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     1441 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1501 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1561 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1621 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1681 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1741 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1801 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1861 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1921 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1981 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     2041 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     2101 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     2161 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     2221 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     2281 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     2341 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     2401 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     2461 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2521 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2581 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2641 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2701 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2761 tttcagaccc acctcccaac cccgagggga cccttgcgcc ttttccaagg cagccctggg
     2821 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
     2881 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
     2941 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
     3001 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
     3061 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
     3121 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
     3181 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
     3241 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggta
     3301 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     3361 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     3421 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     3481 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc
     3541 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     3601 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     3661 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     3721 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     3781 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     3841 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     3901 aagcccggtg cctgagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt
     3961 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     4021 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     4081 tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa
     4141 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     4201 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     4261 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     4321 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     4381 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     4441 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     4501 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     4561 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     4621 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     4681 gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt acaaaaaagt
     4741 tggcatggcc catagatgat tggaactcaa attgttactg agaggttggt ggctctgctg
     4801 gaaagtggaa cggaaaaagt gctgctaatt gatagccggc catttgtgga atacaataca
     4861 tcccacattt tggaagccat taatatcaac tgctccaagc ttatgaagcg aaggttgcaa
     4921 caggacaaag tgttaattac agagctcatc cagcattcag cgaaacataa ggttgacatt
     4981 gattgcagtc agaaggttgt agtttacgat caaagctccc aagatgttgc ctctctctct
     5041 tcagactgtt ttctcactgt acttctgggt aaactggaga agagcttcaa ctctgttcac
     5101 ctgcttgcag gtgggtttgc tgagttctct cgttgtttcc ctggcctctg tgaaggaaaa
     5161 tccactctag tccctacctg catttctcag ccttgcttac ctgttgccaa cattgggcca
     5221 acccgaattc ttcccaatct ttatcttggc tgccagcgag atgtcctcaa caaggagctg
     5281 atgcagcaga atgggattgg ttatgtgtta aatgccagca atacctgtcc aaagcctgac
     5341 tttatccccg agtctcattt cctgcgtgtg cctgtgaatg acagcttttg tgagaaaatt
     5401 ttgccgtggt tggacaaatc agtagatttc attgagaaag caaaagcctc caatggatgt
     5461 gttctagtgc actgtttagc tgggatctcc cgctccgcca ccatcgctat cgcctacatc
     5521 atgaagagga tggacatgtc tttagatgaa gcttacagat ttgtgaaaga aaaaagacct
     5581 actatatctc caaacttcaa ttttctgggc caactcctgg actatgagaa gaagattaag
     5641 aaccagactg gagcatcagg gccaaagagc aaactcaagc tgctgcacct ggagaagcca
     5701 aatgaacctg tccctgctgt ctcagagggt ggacagaaaa gcgagacgcc cctcagtcca
     5761 ccctgtgccg actctgctac ctcagaggca gcaggacaaa ggcccgtgca tcccgccagc
     5821 gtgcccagcg tgcagccgtc gctgttagag gacagcccgc tggtacaggc gctcagtggg
     5881 ctgcacctgt ccgcagacag gctggaagac agcaataagc tcaagcgttc cttctctctg
     5941 gatatcaaat cagtttcata ttcagccagc atggcagcat ccttacatgg cttctcctca
     6001 tcagaagatg ctttggaata ctacaaacct tccactactc tggatgggac caacaagcta
     6061 tgccagttct cccctgttca ggaactatcg gagcagactc ccgaaaccag tcctgataag
     6121 gaggaagcca gcatccccaa gaagctgcag accgccaggc cttcagacag ccagagcaag
     6181 cgattgcatt cggtcagaac cagcagcagt ggcaccgccc agaggtccct tttatctcca
     6241 ctgcatcgaa gtgggagcgt ggaggacaat taccacacca gcttcctttt cggcctttcc
     6301 accagccagc agcacctcac gaagtctgct ggcctgggcc ttaagggctg gcactcggat
     6361 atcttggccc cccagacctc taccccttcc ctgaccagca gctggtattt tgccacagag
     6421 tcctcacact tctactctgc ctcagccatc tacggaggca gtgccagtta ctctgcctac
     6481 agctgcagcc agctgcccac ttgcggagac caagtctatt ctgtgcgcag gcggcagaag
     6541 ccaagtgaca gagctgactc gcggcggagc tggcatgaag agagcccctt tgaaaagcag
     6601 tttaaacgca gaagctgcca aatggaattt ggagagagca tcatgtcaga gaacaggtca
     6661 cgggaagagc tggggaaagt gggcagtcag tctagctttt cgggcagcat ggaaatcatt
     6721 gaggtctcct gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc
     6781 ctcggtctcg attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca
     6841 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6901 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6961 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     7021 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     7081 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     7141 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     7201 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     7261 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     7321 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     7381 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag
     7441 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7501 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     7561 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7621 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7681 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta
     7741 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     7801 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7861 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7921 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     7981 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8041 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8101 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     8161 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     8221 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     8281 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     8341 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     8401 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     8461 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     8521 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     8581 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     8641 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     8701 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     8761 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8821 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8881 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8941 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     9001 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     9061 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     9121 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     9181 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     9241 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     9301 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     9361 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     9421 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     9481 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     9541 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     9601 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     9661 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9721 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9781 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9841 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9901 aga
//
