LOCUS       sequence-174296-       11959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5 tagged human DUSP19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: pLX302-DUSP19-V5 puro
FEATURES             Location/Qualifiers
     source          1..11959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      38..626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(956..983)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1075..1161)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1225..1241
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2009..2033)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2035..2076
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2118..2706
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2171..2191)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2589..2600)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2778..3011
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3089..3210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3126..3145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3180..3199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3250..3385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(3406..3424)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3434..3450)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3592..4047
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3679..3698)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3889..3910
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4073..4177
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4178..4246,4247..5038)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4396..4415)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    6033..6054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6069..6074,6075..6092,6093..6099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6107..6123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6112..6134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6131..6147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6166..6186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6168..6186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6214..6440
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6441..6621
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6668..6793
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7286..7519
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7704..7748
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7923..8423
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8342..8360
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8445..9044
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8445..8464)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8941..8961
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9086..9203
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9320..9623
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9624..9827
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9778..9798
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    9850..9874
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10526..10625)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(10643..10661)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10666..10682)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             11113..11904
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
       61 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      181 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      421 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      481 taagcggcag ggtcggaaca ggagagcgca ckagggagct tccaggggga aacgcctggt
      541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      721 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
      781 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      841 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      901 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
      961 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     1021 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     1081 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     1141 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     1201 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa
     1261 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
     1321 tttttataat gccaactttt gtacaaaaaa gttggcatgt actcccttaa ccaggaaatt
     1381 aaagcattct cccggaataa tctcaggaag caatgcacca gggtgacaac gctaactgga
     1441 aagaaaatta tagaaacatg gaaagatgcc agaattcatg ttgtggaaga agtagagccg
     1501 agcagtgggg gtggttgtgg ttatgtgcag gaccttagct cggacctgca agttggcgtt
     1561 attaagccat ggttgctcct agggtcacaa gatgctgctc atgatttgga tacactgaaa
     1621 aagaataagg tgactcatat tcttaatgtt gcatatggag ttgaaaatgc tttcctcagt
     1681 gactttacat ataagagcat ttctatattg gatctgcctg aaaccaacat cctgtcttat
     1741 tttccagaat gttttgaatt tattgaagaa gcaaaaagaa aagatggagt ggttcttgtt
     1801 cattgtaatg caggcgtttc cagggctgct gcaattgtaa taggtttcct gatgaattct
     1861 gaacaaacct catttaccag tgctttttct ttggtgaaaa atgcaagacc ttccatatgt
     1921 ccaaattctg gcttcatgga gcagcttcgt acatatcaag agggcaaaga aagcaataag
     1981 tgtgacagaa tacaggagaa cagttcatgc ccaactttct tgtacaaagt ggttggtaag
     2041 cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg
     2101 tggcgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2161 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2221 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2281 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2341 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2401 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2461 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     2521 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     2581 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2641 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     2701 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     2761 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     2821 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2881 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     2941 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3001 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     3061 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     3121 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3181 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     3241 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3301 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3361 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     3421 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3481 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3541 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     3601 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3661 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3721 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3781 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3841 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3901 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     3961 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4021 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     4081 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4141 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4201 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4261 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4321 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4381 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4441 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4501 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4561 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4621 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4681 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4741 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4801 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4861 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4921 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4981 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5041 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5101 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5161 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5221 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5281 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5341 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5401 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5461 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5521 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccggaga
     5581 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac tagggagctt
     5641 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5701 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5761 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5821 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5881 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     5941 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     6001 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     6061 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     6121 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     6181 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     6241 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     6301 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     6361 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     6421 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     6481 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6541 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6601 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     6661 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     6721 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     6781 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     6841 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     6901 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     6961 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     7021 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     7081 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     7141 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     7201 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     7261 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     7321 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     7381 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     7441 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     7501 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     7561 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     7621 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     7681 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     7741 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     7801 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     7861 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     7921 ccttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     7981 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     8041 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     8101 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     8161 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     8221 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     8281 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     8341 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     8401 gaatcaccga cctctctccc cagggggtac caccggagct taccatgacc gagtacaagc
     8461 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     8521 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     8581 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     8641 tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     8701 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     8761 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     8821 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     8881 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     8941 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     9001 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagaattc tagatcttga
     9061 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9121 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9181 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     9241 gccggctcga gggggcccgg ggttattaat agtaatcaat tacggggtca ttagttcata
     9301 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     9361 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     9421 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     9481 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     9541 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     9601 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     9661 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     9721 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     9781 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaactg
     9841 tcgggatcaa caagtttgta caaaaaagtt ggcatgtact cccttaacca ggaaattaaa
     9901 gcattctccc ggaataatct caggaagcaa tgcaccaggg tgacaacgct aactggaaag
     9961 aaaattatag aaacatggaa agatgccaga attcatgttg tggaagaagt agagccgagc
    10021 agtgggggtg gttgtggtta tgtgcaggac cttagctcgg acctgcaagt tggcgttatt
    10081 aagccatggt tgctcctagg gtcacaagat gctgctcatg atttggatac actgaaaaag
    10141 aataaggtga ctcatattct taatgttgca tatggagttg aaaatgcttt cctcagtgac
    10201 tttacatata agagcatttc tatattggat ctgcctgaaa ccaacatcct gtcttatttt
    10261 ccagaatgtt ttgaatttat tgaagaagca aaaagaaaag atggagtggt tcttgttcat
    10321 tgtaatgcag gcgtttccag ggctgctgca attgtaatag gtttcctgat gaattctgaa
    10381 caaacctcat ttaccagtgc tttttctttg gtgaaaaatg caagaccttc catatgtcca
    10441 aattctggct tcatggagca gcttcgtaca tatcaagagg gcaaagaaag caataagtgt
    10501 gacagaatac aggagaacag ttcatgccca actttcttgt acaaagttgg cattataaga
    10561 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
    10621 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
    10681 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
    10741 tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag
    10801 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
    10861 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
    10921 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
    10981 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
    11041 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
    11101 aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
    11161 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
    11221 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
    11281 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
    11341 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
    11401 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
    11461 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
    11521 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
    11581 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
    11641 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
    11701 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
    11761 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
    11821 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
    11881 atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg
    11941 agttacgcgt cgttccact
//
