LOCUS       sequence-174297-        8470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5 tagged human JSP1 (DUSP22).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 8470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: pLX302-JSP1-V5 puro
FEATURES             Location/Qualifiers
     source          1..8470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     765..782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    899..920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(935..940,941..958,959..965)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    973..989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     978..1000
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     997..1013
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     997..1013
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1032..1052
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1034..1052
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1080..1306
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1307..1487
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1534..1659
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2152..2385
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2570..2614
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2789..3289
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3208..3226
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3311..3910
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3311..3330)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3807..3827
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3952..4069
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4186..4489
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4490..4693
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4644..4664
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4716..4740
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5293..5317)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5319..5360
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5402..5990
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5455..5475)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5873..5884)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6062..6295
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6373..6494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6410..6429)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6464..6483
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6534..6669
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6689..6708)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6690..6708)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6718..6735)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6718..6734)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6727..6749)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6876..7331
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6963..6982)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7173..7194
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7357..7461
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7462..7530,7531..8322)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7680..7699)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      781 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      841 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      901 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      961 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     1021 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     1081 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     1141 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     1201 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     1261 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     1321 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1381 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1441 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1501 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1561 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1621 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1681 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1741 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1801 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1861 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1921 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1981 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     2041 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     2101 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     2161 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     2221 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     2281 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     2341 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     2401 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     2461 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     2521 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     2581 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     2641 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     2701 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     2761 gacccacctc ccaaccccga ggggaccctt gcgccttttc caaggcagcc ctgggtttgc
     2821 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc
     2881 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
     2941 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
     3001 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
     3061 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
     3121 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
     3181 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
     3241 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg gggtaccacc
     3301 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     3361 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     3421 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     3481 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     3541 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     3601 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     3661 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     3721 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     3781 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     3841 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     3901 cggtgcctga gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     3961 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4021 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     4081 cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt attaatagta
     4141 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     4201 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     4261 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     4321 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     4381 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     4441 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     4501 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     4561 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     4621 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     4681 tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa aaagttggca
     4741 tggggaatgg gatgaacaag atcctgcccg gcctgtacat cggcaacttc aaagatgcca
     4801 gagacgcgga acaattgagc aagaacaagg tgacacatat tctgtctgtc catgatagtg
     4861 ccaggcctat gttggaggga gttaaatacc tgtgcatccc agcagcggat tcaccatctc
     4921 aaaacctgac aagacatttc aaagaaagta ttaaattcat tcacgagtgc cggctccgcg
     4981 gtgagagctg ccttgtacac tgcctggccg gggtctccag gagcgtgaca ctggtgatcg
     5041 catacatcat gaccgtcact gactttggct gggaggatgc cctgcacacc gtgcgtgctg
     5101 ggagatcctg tgccaacccc aacgtgggct tccagagaca gctccaggag tttgagaagc
     5161 atgaggtcca tcagtatcgg cagtggctga aggaagaata tggagagagc cctttgcagg
     5221 atgcagaaga agccaaaaac attctggccg ctccaggaat tctgaagttc tgggcctttc
     5281 tcagaagact gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
     5341 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
     5401 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5461 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5521 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5581 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5641 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5701 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5761 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     5821 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5881 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     5941 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     6001 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6061 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     6121 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6181 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6241 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     6301 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6361 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6421 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6481 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     6541 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6601 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6661 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     6721 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     6781 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     6841 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     6901 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6961 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     7021 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     7081 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     7141 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7201 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     7261 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     7321 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7381 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7441 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7501 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7561 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7621 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7681 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     7741 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7801 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7861 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7921 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7981 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8041 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8101 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8161 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8221 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8281 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8341 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8401 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8461 tcagaccccg
//
