LOCUS       sequence-174323-        8066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral vector encoding cre-dependent expression of kikume.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Lim L, Pakan JMP, Selten MM, Marques-Smith A, Llorca A, Bae SE, 
            Rochefort NL, Marin O
  TITLE     Optimization of interneuron function by direct coupling of cell 
            migration and axonal targeting.
  JOURNAL   Nat Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 
            10.1038/s41593-018-0162-9.
  PUBMED    29915195
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 
            10.1038/s41593-018-0162-9."
COMMENT     Sequence Label: RV-Cag-Dio-kikume
FEATURES             Location/Qualifiers
     source          1..8066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(128..196,197..988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(346..365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1148..1736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1637..1656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1890..1907
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2778..3366
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2884..2895
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3293..3313
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    complement(3376..3409)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3460..3493)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    4287..4320
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4371..4404
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(4447..4466)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          4474..5490
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(4528..4550)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(5491..5766)
                     /label=chicken beta-actin promoter
     enhancer        5768..6147
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6161..6577)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(6177..6193)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6209..6231)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6778..6977
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             7040..7215
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     promoter        complement(7216..7419)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7245..7265)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7420..7799
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 caccaatata aatgtacata ttatgatatg gatacaacgt atgcaatggc caatagccaa
       61 tattgattta tgctatataa ccaatgaata atatggctaa tggccggatc attgaaaaag
      121 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      181 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      241 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      301 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      361 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      421 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      481 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      541 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      601 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      661 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      721 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      781 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      841 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      901 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      961 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1021 gattgatttg cggccgcaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1081 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1261 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1321 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1501 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1741 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1801 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1861 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1921 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc aactatggcc
     1981 atttaatgta aatacttaag aaaaaaaacc aaattaattt tgatacatgc tgcatgtgaa
     2041 agacccccgc tgacgggtag tcaatcactc agaggagacc ctcccaagga acagcgagac
     2101 cacaagtcgg atgcaactgc amgmsgctga cgggtagtca atcactcaga ggagaccctc
     2161 ccaaggaaca gcgagaccac aagtcggatg caactgcaag agggtttatt ggatacacgg
     2221 gtacccgggc gactcagtca atcggaggac tggcgccccg agtgaggggt tgtgggctct
     2281 tttattgagc tcggggagca gaagcgcgcg aacagaagcg agaagcgaac tgattggtta
     2341 gttcaaataa ggcacagggt catttcaggt ccttggggca ccctggaaac atctgatggt
     2401 tctctagaaa ctgctgaggg ctggaccgca tctggggacc atctgttctt ggccctgagc
     2461 cggggcagga actgcttacc acagatatcc tgtttggccc atattcagct gttccatctg
     2521 ttcttggccc tgagccgggg caggaactgc ttaccacaga tatcctgttt ggcccatatt
     2581 cagctgttcc atctgttcct gaccttgatc tgaacttctc tattctcagt tatgtatttt
     2641 tccatgcctt gcaaaatggc gttacttaag ctagcttgcc aaacctacag gtggggtctt
     2701 tcattccccc ctttttctgg agactaaata aaatctttta ttttatcgat gttaacaagc
     2761 tcgagctcga attccaggcg gggaggcggc ccaaagggag atccgactcg tctgagggcg
     2821 aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc
     2881 tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac
     2941 acaggcgagc agccatggaa aggacgtcag cttccccgac aacaccacgg aattgtcagt
     3001 gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt
     3061 ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc
     3121 ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac
     3181 gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa
     3241 agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt
     3301 aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt aatccagagg
     3361 ttgattggtt taaacataac ttcgtatagc atacattata cgaagttatt ctttgcacca
     3421 ttctaaagaa taacagtgat aatttctggg ttaaggcaaa taacttcgta taggatactt
     3481 tatacgaagt tatcgcggcg cgccatggtg agcgtgatca ccagcgagat gaagatcgag
     3541 ctgaggatgg agggctccgt gaacggccac aagttcgtga tcaccggcaa gggcagcggc
     3601 cggccctacg agggcaccca gaccgtggac ctgaccgtga tcgagggcgg ccccctgccc
     3661 ttcgccttcg acatcctgac caccgccttc cactacggca acagggtgtt cgtggagtac
     3721 cccgaggaga tcgtggacta cttcaagcag agcttccccg agggctacag ctgggagagg
     3781 agcatgagct acgaggacgg cggcatctgc ctggccacca acaacatcac catgaagaag
     3841 gacggcagca acaccttcgt gaacgagatc aggttcgacg gcaccaactt ccccgccaac
     3901 ggccccgtga tgcagaggaa gaccgtgaag tgggagccca gcaccgagaa gatgtacgtg
     3961 agggacggcg tgctgaaggg cgacgtggag atggccctgc tgctggaggg cggcggccac
     4021 tacaggtgcg acttcaggac cacctacaag gccaagaagg tggtgcagct gcccgactac
     4081 cactacgtgg accaccagat ggagatcacc agccacgaca aggactacaa caaggtgaag
     4141 gcctacgagc acgccaaggc ctacagcggc acctacaggg gcgccaagta cgagttcgag
     4201 gccaccggta attccgctga cggcggcgga ggatcgggtg gtagtggtgg ttcaggagga
     4261 ggatcgaccc aaggataatt aattaaataa cttcgtataa tgtatgctat acgaagttat
     4321 taagaggttt catattgcta atagcagcta caatccagct accattctgc ataacttcgt
     4381 ataaagtatc ctatacgaag ttattccgga tccgctagcg aattctttgc caaaatgatg
     4441 agacagcaca ataaccagca cgttgcccag gagctgtagg aaaaagaaga aggcatgaac
     4501 atggttagca gaggctctag agccgccggt cacacgccag aagccgaacc ccgccctgcc
     4561 ccgtcccccc cgaaggcagc cgtccccccg cggacagccc cgaggctgga gagggagaag
     4621 gggacggcgg cgcggcgacg cacgaaggcc ctccccgccc atttccttcc tgccggcgcc
     4681 gcaccgcttc gccccgcgcc cgctagaggg ggtgcggcgg cgcctcccag atttcggctc
     4741 cgcacagatt tgggacaaag gaagtccctg cgccctctcg cacgattacc ataaaaggca
     4801 atggctgcgg ctcgccgcgc ctcgacagcc gccggcgctc cgggggccgc cgcgcccctc
     4861 ccccgagccc tccccggccc gaggcggccc cgccccgccc ggcaccccca cctgccgcca
     4921 ccccccgccc ggcacggcga gccccgcgcc acgccccgta cggagccccg cacccgaagc
     4981 cgggccgtgc tcagcaactc ggggaggggg gtgcaggggg ggttgcagcc cgaccgacgc
     5041 gcccacaccc cctgctcacc cccccacgca cacaccccgc acgcagcctt tgttcccctc
     5101 gcagcccccc ccgcaccgcg gggcaccgcc cccggccgcg ctcccctcgc gcacactgcg
     5161 gagcgcacaa agccccgcgc cgcgcccgca gcgctcacag ccgccgggca gcgcggagcc
     5221 gcacgcggcg ctccccacgc acacacacac gcacgcaccc cccgagccgc tccccccgca
     5281 caaagggccc tcccggagcc cctcaaggct ttcacgcagc cacagaaaag aaacaagccg
     5341 tcattaaacc aagcgctaat tacagcccgg aggagaaggg ccgtcccgcc cgctcacctg
     5401 tgggagtaac gcggtcagtc agagccgggg cgggcggcgc gaggcggcgg cggagcgggg
     5461 cacggggcga aggcagcgcg cagcgactcc cgcccgccgc gcgcttcgct ttttataggg
     5521 ccgccgccgc cgccgcctcg ccataaaagg aaactttcgg agcgcgccgc tctgattggc
     5581 tgccgccgca cctctccgcc tcgccccgcc ccgcccctcg ccccgccccg ccccgcctgg
     5641 cgcgcgcccc cccccccccc gcccccatcg ctgcacaaaa taattaaaaa ataaataaat
     5701 acaaaattgg gggtggggag gggggggaga tggggagagt gaagcagaac gtggggctca
     5761 cctcgaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     5821 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     5881 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     5941 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     6001 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     6061 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     6121 attaataact agtcaataat caatgtcgac atcgaattcc ggcgcctaga gaaggagtga
     6181 gggctggata aagggaggat cgaggcgggg tcgaacgagg aggttcaagg gggagagacg
     6241 gggcggatgg aggaagagga ggcggaggct tagggtgtac aaagggcttg acccagggag
     6301 gggggtcaaa agccaaggct tcccaggtca cgatgtaggg gacctggtct gggtgtccat
     6361 gcgggccagg tgaaaagacc ttgatcttaa cctgggtgat gaggtctcgg ttaaaggtgc
     6421 cgtctcgcgg ccatccgacg ttaaaggttg gccattctgc agagcagaag gtaacccaac
     6481 gtctcttctt gacatctacc gactggttgt gagcgatccg ctcgacatct ttccagtgac
     6541 ctaaggtcaa acttaaggga gtggtaacag tctggcccta attttcagac aaatacagaa
     6601 acacagtcag acagagacaa cacagaacga tgctgcagcg ctgcagcaga caagacgcgc
     6661 ggcttcggtt ccaaaccgaa agcaaaaatt cagacggagg cgggaactgt tttaggttct
     6721 cgtctcctac cagaaccaca tatcctgacg gggtcggatt ccacatcgac tcccttcctc
     6781 aggtcgggcc acaaaaacgg cccccaaagt ccctgggacg tctcccaggg ttgcggccgg
     6841 gtgttcagaa ctcgtcagtt ccaccacggg tccgccagat acagagctag ttagctaact
     6901 agtacagacg caggcgcata acatcaaaca tagacactag acaatcggac agacacagat
     6961 aagttgctgg ccagcttacc tcccggtggt gggtcggtgg tccctgggca ggggtctcca
     7021 aatcccggac gagcccccaa atgaaagacc cccgtcgtgg gtagtcaatc actcagagga
     7081 gaccctccca aggaacagcg agaccacgat tcggatgcaa acagcaagag gctttattgg
     7141 gaatacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag tgaggggttg
     7201 tgggctcttt tattgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     7261 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     7321 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     7381 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     7441 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     7501 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     7561 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     7621 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     7681 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     7741 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca
     7801 acatggcggt aatgttggac atgagccaat ataaatgtac atattatgat atggatacaa
     7861 cgtatgcaat ggccaatagc caatattgat ttatgctata taaccaatga ataatatggc
     7921 taatggccgg atcattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     7981 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8041 gtaaaagatg ctgaagatca gttggg
//
