LOCUS       sequence-174332-        6183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hybrid ECA0434[1-385]-Tar[267-553] expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Bi S, Pollard AM, Yang Y, Jin F, Sourjik V
  TITLE     Engineering Hybrid Chemotaxis Receptors in Bacteria.
  JOURNAL   ACS Synth Biol. 2016 Jun 10.
  PUBMED    27285081
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jun 10."
COMMENT     Sequence Label: pSB9
FEATURES             Location/Qualifiers
     source          1..6183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1594..1628
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2047..2706)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2621..2640
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2707..2809)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(3201..3218)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3335..3880)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3415..3434)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccacttggt cagcgacggc ggtattgctc agcgtctggc agcctattac aacctgctga
       61 acgtcaaaga acaggcaggg cttgaacgcg ccgtgctttc taataccttc tctgccaata
      121 gttttgctac cgggatgttc gagcgtctga accagatggt aggtaaggga gaggcgtata
      181 tcaccgccta taacatgttc gccaatgccg agctgcgtaa agcttttgaa caggctctta
      241 acaaccctgc ggcccagaac gcactccaga tgcgcaataa ggctatcgct tctcccggcg
      301 gcaatttctc catcgatgcc aaccagtggt ttaaccaaca aacagtaaaa atcgatgagc
      361 tgaagaacgt tgagcagctt gccaccaacg atctgacggc acaggttaac gccttagccg
      421 ccgatgcacg tcaatcctgg attagctatt tggctggtgc gttgatttct ctcctgatgg
      481 cgctgggtct ggcttcgatg attatgcgca gtatcaacga gcagcttcag caaaccctga
      541 cgaccattcg tgagatgggc ggcgatctca cccgtcgcct gcgcgtaccg ggaaccgacg
      601 agctttcaca gctgaatcag gcctataacg cctcactgga aaacatcgct gacatggtgg
      661 tgaatcatgt ccgcgaaggt tcagatgcca tctatgccgg tacccgtgaa attgcggcgg
      721 gcaacaccga tctttcctcc cgtactgaac agcaggcatc cgcgctggaa gaaactgccg
      781 ccagcatgga gcagctcacc gcgacagtga agcaaaacgc cgataacgcc cgccaggcct
      841 cgcaactggc gcaaagtgcc tccgacaccg cccagcacgg cggcaaagtg gtggatggcg
      901 tagtgaaaac gatgcatgag atcgccgata gttcgaagaa aattgccgac attatcagcg
      961 ttatcgacgg tattgccttc cagactaata tcctcgcgct gaatgccgcg gttgaagccg
     1021 cgcgtgcggg tgaacagggc cgtggttttg ccgtggtggc gggtgaagtg cgtaatcttg
     1081 ccagtcgcag cgcccaggcg gcaaaagaga tcaaagccct cattgaagac tccgtctcac
     1141 gcgttgatac cggttcggtg ctggtcgaaa gcgccgggga aacaatgaac aatatcgtca
     1201 atgctgtcac tcgcgtgact gacattatgg gcgagattgc atcggcatcg gatgaacaga
     1261 gccgtggcat cgatcaagtc gcattggcgg tttcggaaat ggatcgcgtc acgcaacaga
     1321 acgcatcgct ggtgcaggaa tcagctgccg ccgccgctgc gctggaagaa caggcgagtc
     1381 gtttaacgca agcagtttcc gcgttccgtc tggcagccag cccactcacc aataaaccgc
     1441 aaacaccatc ccgtcctgcc agtgagcaac caccggctca gccacgactg cgaattgctg
     1501 aacaagatcc aaactgggaa acattttgag gatccgcgga taaataagta acgatccggt
     1561 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     1621 ttttttattg gtgagaatcg cagcaacttg tcgcgccaat cgagccatgt cgtcgtcaac
     1681 gaccccccat tcaagaacag caagcagcat tgagaacttt ggaatccagt ccctcttcca
     1741 cctgctgacc ggatcagcag tccccggaac atcgtagctg acgccttcgc gttgctcagt
     1801 tgtccaaccc cggaaacggg aaaaagcaag ttttccccgc tcccggcgtt tcaataactg
     1861 aaaaccatac tatttcacag tttaaatcac attaaacgac agtaatcccc gttgatttgt
     1921 gcgccaacac agatccgtcg accgggtcga atttgctttc gaatttctgc cattcatccg
     1981 cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta
     2041 aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     2101 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     2161 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     2221 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     2281 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     2341 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     2401 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     2461 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
     2521 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     2581 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     2641 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     2701 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     2761 tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt
     2821 tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc
     2881 tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc
     2941 caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta
     3001 tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg
     3061 gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag
     3121 tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga
     3181 tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
     3241 cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt
     3301 aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca
     3361 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
     3421 accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     3481 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
     3541 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc
     3601 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca
     3661 ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta
     3721 aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt
     3781 caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt
     3841 ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca
     3901 gataaaatat ttctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag
     3961 ccccatacga tataagttgt aattctcatg tttgacagct tatcatcgat cccgcaagag
     4021 gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag
     4081 gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa
     4141 ctgtgataaa ctaccgcatt aaagcttctt gcgaaacagc tctcgaccag gtttatactg
     4201 gtcgtgcaga tgaaagcagt ttttgccgag gtcgattccg accagcgcca tatcgctcat
     4261 agtgatggtc tccgaattat aaacaccctg cgaaagcgta gcctacgcag ggtgtcgggt
     4321 gctcatccca ttacccgttt tcccatctct taacttaagg ggggaacggt gtcctgtctt
     4381 tcatggggct cgttcactgc taacttacct gcttccctgt ctgtcaattc tctctatcct
     4441 gcgatcccgc gaagaaccaa aaaagctcga cagagggcgc ggtcatttta ggtcgggcgg
     4501 atcggcgccg ccggctcggc tggtgtgccg cacagcaccg cctacgtgag ctgccagttg
     4561 atgaacttcc cccgttgcca gctagggcgc aagcgggctg tataagatca ctgcccatca
     4621 cattgatcgg ctcggatttt ttctcaatcc gtaaacaggt caaacatcag ttgccgcaac
     4681 caaatattgg ctaggtcctt gtggtacttc gcatgccaga acatgttgat ggctatttca
     4741 ggcaagacga ctgggtgcgg caaggcgctt aggccgaagg gctccacaca gcagtcggct
     4801 aaacgtatcg gcacagtggc gagcagatcg gtgcgctgga ggatgtggcc aacggcggcg
     4861 aagtgcggca cttccagacg gatgtcgcgc cggatgccga cccgtgtcat gtacgtgtcc
     4921 acctcgccgt ggccggtgcc agcggcgatg acacgcacgt ggccgtagga acagaagcgc
     4981 tccagagtca ggggttcgcg ggtgactgga tggtccttgc gacataggca cacgtagtga
     5041 ttctggagca gccggcgctg aaagaagcca gtttgcagat tgggaagcag gcccacggcc
     5101 aagtccacgg ttccgttctg caaggcctgc atcaggctca tcgaactgtc gcgcaccgta
     5161 ctgatcacgc aattgggggc ctggtgagcc agcacatcca tcagccgcgg catgaagtag
     5221 atctcgccaa tgtcggtcat ggccagggtg aaggtacgct cgctggtcag cggatcgaag
     5281 ctttcatggt gctgtagggc gttgcgcagt gcgtgcatgg ccgaagtgac gggctcggcc
     5341 agatgcgcgg catagggtgt gggttccatt ccctgatgtg tgcgcacgaa gagtgggtcc
     5401 tgtagcgagg tgcgcaggcg tttcagcgca ttgctcacgg caggctgggt caggcccagg
     5461 ttctccgcag tgatagagac gcgtctgtcg accagcaact ggttgaacac caccagcagg
     5521 tttaaatcca ggtcacgcag ttccatgggg cctcgcttgg gttattgctg gtgcccggcc
     5581 gggcgcaata ttcatgttga tgatttatta tatatcgagt ggtgtattta tcaatattgt
     5641 ttgctccgtt atcgttatta acaagtcatc aataatgcca taaggagtac catatgaaat
     5701 cccttcatca catatcgatc cgtagtaagt tcattctcgc cctcctcccg cccatccttg
     5761 ctctgctatg gttcagtttt tccggcgtga tggagcgacg tagcacagag catgagatga
     5821 tccgtatgga gaaactgatt acgctagcac gtgacgcagg cgagttagcc caccagctcc
     5881 agcgtgaacg cggaatgagc gcaggttatt ttggcagcca agggaagaag tttggtgccg
     5941 aactcactac gcagcggcaa gccagcgatc gggcacagca acagtttcaa caaacggctg
     6001 cgaatctcag tgacagtgaa ctcggtaacg atgtgagtgg cgagatcggt aaaattacgc
     6061 agaaaatgca acagcttgac gagtatcgtc gcaatatcga cagcatgtcg atctctgtca
     6121 cgcaggcact cggctactat tccgattccg tcagctatct gctgagtatt gtcggagata
     6181 tga
//
