LOCUS       sequence-174333-        5763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hybrid Tar[1-43]-CitA[55-173]-Tar[184-553] expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Bi S, Pollard AM, Yang Y, Jin F, Sourjik V
  TITLE     Engineering Hybrid Chemotaxis Receptors in Bacteria.
  JOURNAL   ACS Synth Biol. 2016 Jun 10.
  PUBMED    27285081
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jun 10."
COMMENT     Sequence Label: pSB7
FEATURES             Location/Qualifiers
     source          1..5763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      301..335
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(754..1413)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1328..1347
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1414..1516)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1908..1925)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2042..2587)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2122..2141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggaaatgga tcgcgtcacg caacagaacg catcgctggt gcaggaatca gctgccgccg
       61 ccgctgcgct ggaagaacag gcgagtcgtt taacgcaagc agtttccgcg ttccgtctgg
      121 cagccagccc actcaccaat aaaccgcaaa caccatcccg tcctgccagt gagcaaccac
      181 cggctcagcc acgactgcga attgctgaac aagatccaaa ctgggaaaca ttttgaggat
      241 ccgcggataa ataagtaacg atccggtcca gtaatgacct cagaactcca tctggatttg
      301 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatcgcag caacttgtcg
      361 cgccaatcga gccatgtcgt cgtcaacgac cccccattca agaacagcaa gcagcattga
      421 gaactttgga atccagtccc tcttccacct gctgaccgga tcagcagtcc ccggaacatc
      481 gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg aaacgggaaa aagcaagttt
      541 tccccgctcc cggcgtttca ataactgaaa accatactat ttcacagttt aaatcacatt
      601 aaacgacagt aatccccgtt gatttgtgcg ccaacacaga tccgtcgacc gggtcgaatt
      661 tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt agcaaccagg
      721 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca
      781 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      841 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      901 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
      961 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     1021 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1081 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1141 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc
     1201 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1261 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1321 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1381 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1441 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1501 tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc
     1561 aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc
     1621 ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
     1681 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
     1741 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta
     1801 ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct
     1861 gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac
     1921 tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat
     1981 ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt
     2041 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
     2101 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc
     2161 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt
     2221 ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg
     2281 cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2341 aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga
     2401 gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg
     2461 cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa
     2521 accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg
     2581 atctcaagaa gatcatctta ttaatcagat aaaatatttc tagatttcag tgcaatttat
     2641 ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgttt
     2701 gacagcttat catcgatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc
     2761 tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca
     2821 cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttcttgcg
     2881 aaacagctct cgaccaggtt tatactggtc gtgcagatga aagcagtttt tgccgaggtc
     2941 gattccgacc agcgccatat cgctcatagt gatggtctcc gaattataaa caccctgcga
     3001 aagcgtagcc tacgcagggt gtcgggtgct catcccatta cccgttttcc catctcttaa
     3061 cttaaggggg gaacggtgtc ctgtctttca tggggctcgt tcactgctaa cttacctgct
     3121 tccctgtctg tcaattctct ctatcctgcg atcccgcgaa gaaccaaaaa agctcgacag
     3181 agggcgcggt cattttaggt cgggcggatc ggcgccgccg gctcggctgg tgtgccgcac
     3241 agcaccgcct acgtgagctg ccagttgatg aacttccccc gttgccagct agggcgcaag
     3301 cgggctgtat aagatcactg cccatcacat tgatcggctc ggattttttc tcaatccgta
     3361 aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg gtacttcgca
     3421 tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa ggcgcttagg
     3481 ccgaagggct ccacacagca gtcggctaaa cgtatcggca cagtggcgag cagatcggtg
     3541 cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat gtcgcgccgg
     3601 atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc ggcgatgaca
     3661 cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt gactggatgg
     3721 tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa gaagccagtt
     3781 tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa ggcctgcatc
     3841 aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg gtgagccagc
     3901 acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc cagggtgaag
     3961 gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt gcgcagtgcg
     4021 tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg ttccattccc
     4081 tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt cagcgcattg
     4141 ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg tctgtcgacc
     4201 agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc catggggcct
     4261 cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga tttattatat
     4321 atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca agtcatcaat
     4381 aaagccataa ggagtaccat atgattaacc gtatccgcgt agtcacgctg ttggtaatgg
     4441 tgctgggggt attcgcactg ttacagctta tttccggcag tctgtttttt tcttcccttc
     4501 accatagcca gaagagcttt gtggtttccg acatggcaat gaatcaggcg aaaattattg
     4561 cctccaatga cagtgtcatc tctgcggtga aaacgcgtga ctacaaacgg ctggcgacca
     4621 tcgctaacaa attacaaaga gataccgatt ttgattatgt ggtgattggg gaccggcact
     4681 cgatccgcct ttaccatcct aatccggaga aaattggtta tcctatgcag ttcaccaaac
     4741 agggcgcgct ggagaaaggg gagagctact tcattaccgg gaaagggtca atggggatgg
     4801 cgatgcgcgc caaaacgcca atctttgatg acgatggaaa agtcatcggc gtggtgtcga
     4861 ttggctacct ggtgagtaaa atcgatgcag atgattaccg atttgcccag tggcaactgg
     4921 cggttatcgc gctggtggtg gtattgattc tgctggtggc gtggtacggc attcgccgta
     4981 tgttgcttac tccgctggca aaaattattg ctcacattcg cgaaatcgcc ggtggtaacc
     5041 tggcgaatac cctgaccatt gacgggcgca gtgaaatggg cgacctggcg cagagcgttt
     5101 cacacatgca acgctctttg actgacaccg tcactcatgt ccgcgaaggt tcagatgcca
     5161 tctatgccgg tacccgtgaa attgcggcgg gcaacaccga tctttcctcc cgtactgaac
     5221 agcaggcatc cgcgctggaa gaaattgccg ccagcatgga gcagctcacc gcgacagtga
     5281 agcaaaacgc cgataacgcc cgccaggcct cgcaactggc gcaaagtgcc tccgacaccg
     5341 cccagcacgg cggcaaagtg gtggatggcg tagtgaaaac gatgcatgag atcgccgata
     5401 gttcgaagaa aattgccgac attatcagcg ttatcgacgg tattgccttc cagactaata
     5461 tcctcgcgct gaatgccgcg gttgaagccg cgcgtgcggg tgaacagggc cgtggttttg
     5521 ccgtggtggc gggtgaagtg cgtaatcttg ccagtcgcag cgcccaggcg gcaaaagaga
     5581 tcaaagccct cattgaagac tccgtctcac gcgttgatac cggttcggtg ctggtcgaaa
     5641 gcgccgggga aacaatgaac aatatcgtca atgctgtcac tcgcgtgact gacattatgg
     5701 gcgagattgc atcggcatcg gatgaacaga gccgtggcat cgatcaagtc gcattggcgg
     5761 ttt
//
