LOCUS       sequence-174335-        5796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hybrid CitA[1-193]-Tar[204-553] expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Bi S, Pollard AM, Yang Y, Jin F, Sourjik V
  TITLE     Engineering Hybrid Chemotaxis Receptors in Bacteria.
  JOURNAL   ACS Synth Biol. 2016 Jun 10.
  PUBMED    27285081
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jun 10."
COMMENT     Sequence Label: pSB8
FEATURES             Location/Qualifiers
     source          1..5796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      521..555
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(974..1633)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1548..1567
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1634..1736)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(2128..2145)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2262..2807)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2342..2361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatcttgcca gtcgcagcgc ccaggcggca aaagagatca aagccctcat tgaagactcc
       61 gtctcacgcg ttgataccgg ttcggtgctg gtcgaaagcg ccggggaaac aatgaacaat
      121 atcgtcaatg ctgtcactcg cgtgactgac attatgggcg agattgcatc ggcatcggat
      181 gaacagagcc gtggcatcga tcaagtcgca ttggcggttt cggaaatgga tcgcgtcacg
      241 caacagaacg catcgctggt gcaggaatca gctgccgccg ccgctgcgct ggaagaacag
      301 gcgagtcgtt taacgcaagc agtttccgcg ttccgtctgg cagccagccc actcaccaat
      361 aaaccgcaaa caccatcccg tcctgccagt gagcaaccac cggctcagcc acgactgcga
      421 attgctgaac aagatccaaa ctgggaaaca ttttgaggat ccgcggataa ataagtaacg
      481 atccggtcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
      541 ccgggcgttt tttattggtg agaatcgcag caacttgtcg cgccaatcga gccatgtcgt
      601 cgtcaacgac cccccattca agaacagcaa gcagcattga gaactttgga atccagtccc
      661 tcttccacct gctgaccgga tcagcagtcc ccggaacatc gtagctgacg ccttcgcgtt
      721 gctcagttgt ccaaccccgg aaacgggaaa aagcaagttt tccccgctcc cggcgtttca
      781 ataactgaaa accatactat ttcacagttt aaatcacatt aaacgacagt aatccccgtt
      841 gatttgtgcg ccaacacaga tccgtcgacc gggtcgaatt tgctttcgaa tttctgccat
      901 tcatccgctt attatcactt attcaggcgt agcaaccagg cgtttaaggg caccaataac
      961 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     1021 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     1081 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1141 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     1201 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1261 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1321 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1381 atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc
     1441 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1501 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1561 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1621 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1681 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     1741 ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta
     1801 tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg
     1861 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct
     1921 gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc
     1981 accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg
     2041 gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa
     2101 tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     2161 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa
     2221 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc
     2281 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
     2341 taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
     2401 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
     2461 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     2521 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
     2581 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
     2641 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
     2701 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
     2761 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
     2821 ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg
     2881 aagtcagccc catacgatat aagttgtaat tctcatgttt gacagcttat catcgatccc
     2941 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
     3001 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
     3061 aatttaactg tgataaacta ccgcattaaa gcttcttgcg aaacagctct cgaccaggtt
     3121 tatactggtc gtgcagatga aagcagtttt tgccgaggtc gattccgacc agcgccatat
     3181 cgctcatagt gatggtctcc gaattataaa caccctgcga aagcgtagcc tacgcagggt
     3241 gtcgggtgct catcccatta cccgttttcc catctcttaa cttaaggggg gaacggtgtc
     3301 ctgtctttca tggggctcgt tcactgctaa cttacctgct tccctgtctg tcaattctct
     3361 ctatcctgcg atcccgcgaa gaaccaaaaa agctcgacag agggcgcggt cattttaggt
     3421 cgggcggatc ggcgccgccg gctcggctgg tgtgccgcac agcaccgcct acgtgagctg
     3481 ccagttgatg aacttccccc gttgccagct agggcgcaag cgggctgtat aagatcactg
     3541 cccatcacat tgatcggctc ggattttttc tcaatccgta aacaggtcaa acatcagttg
     3601 ccgcaaccaa atattggcta ggtccttgtg gtacttcgca tgccagaaca tgttgatggc
     3661 tatttcaggc aagacgactg ggtgcggcaa ggcgcttagg ccgaagggct ccacacagca
     3721 gtcggctaaa cgtatcggca cagtggcgag cagatcggtg cgctggagga tgtggccaac
     3781 ggcggcgaag tgcggcactt ccagacggat gtcgcgccgg atgccgaccc gtgtcatgta
     3841 cgtgtccacc tcgccgtggc cggtgccagc ggcgatgaca cgcacgtggc cgtaggaaca
     3901 gaagcgctcc agagtcaggg gttcgcgggt gactggatgg tccttgcgac ataggcacac
     3961 gtagtgattc tggagcagcc ggcgctgaaa gaagccagtt tgcagattgg gaagcaggcc
     4021 cacggccaag tccacggttc cgttctgcaa ggcctgcatc aggctcatcg aactgtcgcg
     4081 caccgtactg atcacgcaat tgggggcctg gtgagccagc acatccatca gccgcggcat
     4141 gaagtagatc tcgccaatgt cggtcatggc cagggtgaag gtacgctcgc tggtcagcgg
     4201 atcgaagctt tcatggtgct gtagggcgtt gcgcagtgcg tgcatggccg aagtgacggg
     4261 ctcggccaga tgcgcggcat agggtgtggg ttccattccc tgatgtgtgc gcacgaagag
     4321 tgggtcctgt agcgaggtgc gcaggcgttt cagcgcattg ctcacggcag gctgggtcag
     4381 gcccaggttc tccgcagtga tagagacgcg tctgtcgacc agcaactggt tgaacaccac
     4441 cagcaggttt aaatccaggt cacgcagttc catggggcct cgcttgggtt attgctggtg
     4501 cccggccggg cgcaatattc atgttgatga tttattatat atcgagtggt gtatttatca
     4561 atattgtttg ctccgttatc gttattaaca agtcatcaat aatgccataa ggagtaccat
     4621 atgttgcagc ttaacgagaa taaacagttt gcatttttcc aaagactggc atttccgctg
     4681 cgtatctttt tgctgattct ggtgttctca atatttgtca ttgcagccct ggcgcaatat
     4741 tttacggcca gttttgagga ctatttaacg cttcatgtac gcgacatggc aatgaatcag
     4801 gcgaaaatta ttgcctccaa tgacagtgtc atctctgcgg tgaaaacgcg tgactacaaa
     4861 cggctggcga ccatcgctaa caaattacaa agagataccg attttgatta tgtggtgatt
     4921 ggggaccggc actcgatccg cctttaccat cctaatccgg agaaaattgg ttatcctatg
     4981 cagttcacca aacagggcgc gctggagaaa ggggagagct acttcattac cgggaaaggg
     5041 tcaatgggga tggcgatgcg cgccaaaacg ccaatctttg atgacgatgg aaaagtcatc
     5101 ggcgtggtgt cgattggcta cctggtgagt aaaatcgata gctggcgggc tgagttttta
     5161 ttaccgatgg caggtgtgtt tgtcgtgctg ttagggatta ttctgctggt ggcgtggtac
     5221 ggcattcgcc gtatgttgct tactccgctg gcaaaaatta ttgctcacat tcgcgaaatc
     5281 gccggtggta acctggcgaa taccctgacc attgacgggc gcagtgaaat gggcgacctg
     5341 gcgcagagcg tttcacacat gcaacgctct ttgactgaca ccgtcactca tgtccgcgaa
     5401 ggttcagatg ccatctatgc cggtacccgt gaaattgcgg cgggcaacac cgatctttcc
     5461 tcccgtactg aacagcaggc atccgcgctg gaagaaactg ccgccagcat ggagcagctc
     5521 accgcgacag tgaagcaaaa cgccgataac gcccgccagg cctcgcaact ggcgcaaagt
     5581 gcctccgaca ccgcccagca cggcggcaaa gtggtggatg gcgtagtgaa aacgatgcat
     5641 gagatcgccg atagttcgaa gaaaattgcc gacattatca gcgttatcga cggtattgcc
     5701 ttccagacta atatcctcgc gctgaatgcc gcggttgaag ccgcgcgtgc gggtgaacag
     5761 ggccgtggtt ttgccgtggt ggcgggtgaa gtgcgt
//
