LOCUS       sequence-174336-        6114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hybrid McpS[1-295]-Tar[200-553] expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Bi S, Pollard AM, Yang Y, Jin F, Sourjik V
  TITLE     Engineering Hybrid Chemotaxis Receptors in Bacteria.
  JOURNAL   ACS Synth Biol. 2016 Jun 10.
  PUBMED    27285081
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jun 10."
COMMENT     Sequence Label: pSB10
FEATURES             Location/Qualifiers
     source          1..6114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      406..440
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(859..1518)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1433..1452
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1519..1621)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(2013..2030)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2147..2692)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2227..2246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acaatatcgt caatgctgtc actcgcgtga ctgacattat gggcgagatt gcatcggcat
       61 cggatgaaca gagccgtggc atcgatcaag tcgcattggc ggtttcggaa atggatcgcg
      121 tcacgcaaca gaacgcatcg ctggtgcagg aatcagctgc cgccgccgct gcgctggaag
      181 aacaggcgag tcgtttaacg caagcagttt ccgcgttccg tctggcagcc agcccactca
      241 ccaataaacc gcaaacacca tcccgtcctg ccagtgagca accaccggct cagccacgac
      301 tgcgaattgc tgaacaagat ccaaactggg aaacattttg aggatccgcg gataaataag
      361 taacgatccg gtccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt
      421 tgccgccggg cgttttttat tggtgagaat cgcagcaact tgtcgcgcca atcgagccat
      481 gtcgtcgtca acgacccccc attcaagaac agcaagcagc attgagaact ttggaatcca
      541 gtccctcttc cacctgctga ccggatcagc agtccccgga acatcgtagc tgacgccttc
      601 gcgttgctca gttgtccaac cccggaaacg ggaaaaagca agttttcccc gctcccggcg
      661 tttcaataac tgaaaaccat actatttcac agtttaaatc acattaaacg acagtaatcc
      721 ccgttgattt gtgcgccaac acagatccgt cgaccgggtc gaatttgctt tcgaatttct
      781 gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca
      841 ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
      901 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
      961 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     1021 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     1081 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     1141 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     1201 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     1261 atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat
     1321 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     1381 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     1441 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     1501 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     1561 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     1621 aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg
     1681 atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
     1741 gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg
     1801 cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca
     1861 aaagcaccgc cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga
     1921 tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag
     1981 cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc
     2041 gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc
     2101 aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc
     2161 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
     2221 gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg
     2281 cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg
     2341 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     2401 cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat
     2461 gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc
     2521 atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca
     2581 gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc
     2641 ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca
     2701 tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc
     2761 acctgaagtc agccccatac gatataagtt gtaattctca tgtttgacag cttatcatcg
     2821 atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt
     2881 gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg
     2941 ttagcaattt aactgtgata aactaccgca ttaaagcttc ttgcgaaaca gctctcgacc
     3001 aggtttatac tggtcgtgca gatgaaagca gtttttgccg aggtcgattc cgaccagcgc
     3061 catatcgctc atagtgatgg tctccgaatt ataaacaccc tgcgaaagcg tagcctacgc
     3121 agggtgtcgg gtgctcatcc cattacccgt tttcccatct cttaacttaa ggggggaacg
     3181 gtgtcctgtc tttcatgggg ctcgttcact gctaacttac ctgcttccct gtctgtcaat
     3241 tctctctatc ctgcgatccc gcgaagaacc aaaaaagctc gacagagggc gcggtcattt
     3301 taggtcgggc ggatcggcgc cgccggctcg gctggtgtgc cgcacagcac cgcctacgtg
     3361 agctgccagt tgatgaactt cccccgttgc cagctagggc gcaagcgggc tgtataagat
     3421 cactgcccat cacattgatc ggctcggatt ttttctcaat ccgtaaacag gtcaaacatc
     3481 agttgccgca accaaatatt ggctaggtcc ttgtggtact tcgcatgcca gaacatgttg
     3541 atggctattt caggcaagac gactgggtgc ggcaaggcgc ttaggccgaa gggctccaca
     3601 cagcagtcgg ctaaacgtat cggcacagtg gcgagcagat cggtgcgctg gaggatgtgg
     3661 ccaacggcgg cgaagtgcgg cacttccaga cggatgtcgc gccggatgcc gacccgtgtc
     3721 atgtacgtgt ccacctcgcc gtggccggtg ccagcggcga tgacacgcac gtggccgtag
     3781 gaacagaagc gctccagagt caggggttcg cgggtgactg gatggtcctt gcgacatagg
     3841 cacacgtagt gattctggag cagccggcgc tgaaagaagc cagtttgcag attgggaagc
     3901 aggcccacgg ccaagtccac ggttccgttc tgcaaggcct gcatcaggct catcgaactg
     3961 tcgcgcaccg tactgatcac gcaattgggg gcctggtgag ccagcacatc catcagccgc
     4021 ggcatgaagt agatctcgcc aatgtcggtc atggccaggg tgaaggtacg ctcgctggtc
     4081 agcggatcga agctttcatg gtgctgtagg gcgttgcgca gtgcgtgcat ggccgaagtg
     4141 acgggctcgg ccagatgcgc ggcatagggt gtgggttcca ttccctgatg tgtgcgcacg
     4201 aagagtgggt cctgtagcga ggtgcgcagg cgtttcagcg cattgctcac ggcaggctgg
     4261 gtcaggccca ggttctccgc agtgatagag acgcgtctgt cgaccagcaa ctggttgaac
     4321 accaccagca ggtttaaatc caggtcacgc agttccatgg ggcctcgctt gggttattgc
     4381 tggtgcccgg ccgggcgcaa tattcatgtt gatgatttat tatatatcga gtggtgtatt
     4441 tatcaatatt gtttgctccg ttatcgttat taacaagtca tcaataatgc cataaggagt
     4501 accatatgaa cagctggttc gccaacatca gcgtcaacct taaactcggc ctgggcttcg
     4561 gcctggtgct ggtcctcact ggcctgctgg ccctcaccgg ctggaccagc ctgggcagcc
     4621 tgatcgaccg tagcaactgg atgggcgata ttggccagtt gaacaaggac ctcaccgatc
     4681 tacgcattgc gcgcctgcag tacatgatcg ccaacggcga cgataccgcc gccgccaaca
     4741 ccctggccaa gctggacgca ttcagcaagc agcaggccta cctggccacc accttcaaaa
     4801 gcccggagaa cgtcaagctg ctgggtgagc tgggcgatac catcagtgcc tacaagctgt
     4861 cgctgaacaa gatgcgccag ggctacgacg ccacccgcgc agcgcgcgtt tccatggaca
     4921 gttcggccat tcgcgccgac caggccatgg atgcgctcag ccaggaggtg atggctcgcc
     4981 ccgaggctga cagcgtgcgc ctggcccagt accagctgat cagcaaggcc cgccagcaac
     5041 tgctgcaggt acgcatcgat gtgcgtggct acatcgccga gaacagcagc gccaacgagc
     5101 aggccgcctt gcgccagctg gatgcagcac tggccgatac cgacaacctc aagcgccagc
     5161 tgcccagcga agacgcccgc ctgcaacagt tcgaaaacgc agtactggcc tatcgtgacg
     5221 ccgtacgcca gttccgcgac gcggttgcca acatcaccac ctcgcgcgcc gaaatgaccg
     5281 tgcagggcgc cgacatcgtc aagcgcagtg acgcgttgta ccagatccag ctggagcgcc
     5341 gtgacatcga gagcacccag gcccgcagcc tgcaggccat cgccacactg gcggccgcat
     5401 tgattctgct ggtggcgtgg tacggcattc gccgtatgtt gcttactccg ctggcaaaaa
     5461 ttattgctca cattcgcgaa atcgccggtg gtaacctggc gaataccctg accattgacg
     5521 ggcgcagtga aatgggcgac ctggcgcaga gcgtttcaca catgcaacgc tctttgactg
     5581 acaccgtcac tcatgtccgc gaaggttcag atgccatcta tgccggtacc cgtgaaattg
     5641 cggcgggcaa caccgatctt tcctcccgta ctgaacagca ggcatccgcg ctggaagaaa
     5701 ctgccgccag catggagcag ctcaccgcga cagtgaagca aaacgccgat aacgcccgcc
     5761 aggcctcgca actggcgcaa agtgcctccg acaccgccca gcacggcggc aaagtggtgg
     5821 atggcgtagt gaaaacgatg catgagatcg ccgatagttc gaagaaaatt gccgacatta
     5881 tcagcgttat cgacggtatt gccttccaga ctaatatcct cgcgctgaat gccgcggttg
     5941 aagccgcgcg tgcgggtgaa cagggccgtg gttttgccgt ggtggcgggt gaagtgcgta
     6001 atcttgccag tcgcagcgcc caggcggcaa aagagatcaa agccctcatt gaagactccg
     6061 tctcacgcgt tgataccggt tcggtgctgg tcgaaagcgc cggggaaaca atga
//
