LOCUS       sequence-174337-       14390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disturb the expression of miR408-5p in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14390)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM408-5p
FEATURES             Location/Qualifiers
     source          1..14390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    506..682
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1244..1262)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1245..1262)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1901..1920
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2023..2028,2029..2045,2046..2051))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2310..2327)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2310..2326)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2319..2341)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2703..3048
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3065..3079,3270..5087)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          3080..3269
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             5094..5111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5146..5398
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5420..5444
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6745..7374
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7803..8876
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8942..9136
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9372..9394)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9480..9620
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9535..9554
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9635..9652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9806..10394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10481..11275)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11700..11724
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11802..11976
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12017..13042)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(13115..13460)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    13651..13672
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13687..13692,13693..13710,13711..13717)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13725..13741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13730..13752
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13749..13765
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13749..13765
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13965..13984
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13965..13983
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacccc atcgaaagga cagtagaaaa ggagggtggc tcctacaaat gccatcattg
      121 cgataaaggg aaggctatca ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacaccca agcttccatg
      361 ctctgcctac tcatccctgg ttgttgttgt tatggtctaa tttaaatatg gtctaaagaa
      421 gaagaatcca tgctctgcct actcatccct ggaattcggt acgctgaaat caccagtctc
      481 tctctacaaa tctatctctc tctattttct ccataaataa tgtgtgagta gtttcccgat
      541 aagggaaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
      601 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
      661 atccagtact aaaatccaga tctcctaaag tccctataga tctttgtcgt gaatataaac
      721 cggacacgag acgactagac ctggagccca gacgccgttc gaagctagaa gtaccgctta
      781 ggcaggaggc cgttagggaa aagatgctaa ggcagggttg gttacgttga ctcccccgta
      841 ggtttggttt aaatatgatg aagtggacgg aaggaaggag gaagacaagg aaggataagg
      901 ttgcaggccc tgtgcaaggt aagaagatgg aaatttgata gaggtacgct actatactta
      961 tactatacgc taagggaatg attgtattta taccctatac cccctaataa ccccttatca
     1021 atttaagaaa taatccgcat aagcccccgc ttaaaaattg gtatcagagc catgaatagg
     1081 tctatgacca aaactcaaga ggataaaacc tcaccaaaat acgaaagagt ccttaactct
     1141 aaagataaaa gatctttcaa gatcaaaact agttccctca caccggtgac ggggatcgca
     1201 tgcgacctct agaccatcac attggagctt cagctgctcg agttctatag tgtcacctaa
     1261 atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct tacgtcgacg
     1321 taccaattac gccccgccct gtcactcatc gcagtactgt tgtaattcat taagcattct
     1381 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1441 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1501 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1561 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1621 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1681 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatattac
     1741 cagctcaccg tctttcatcg ccatacggaa ctccggatga gcattcatca ggcgggcaag
     1801 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1861 cgtaatatcc agctgaacgg cctggttata ggtacattga gcaactgact gaaatgcctc
     1921 aaaatgctct ttacgatgcc attgggatat atcaacggtg gtatatccag tgatttcttt
     1981 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcattaaagc ttatcgatga
     2041 taagctgtca aacatgagaa tttagcccgc ctaatgagcg ggctttttgt tgtgatccgc
     2101 gttgctggcg ttttcccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2161 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc ccccctggaa
     2221 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2281 ttaattaact gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
     2341 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     2401 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     2461 gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca gtttagcttc
     2521 atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac tggcgaacag
     2581 ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
     2641 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
     2701 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
     2761 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
     2821 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
     2881 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     2941 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     3001 caagaccctt cctctatata aggaagttca tttcatttgg agagaacacg ggggactctt
     3061 gaccatggta gatctgaggg taaatttcta gtttttctcc ttcattttct tggttaggac
     3121 ccttttctct ttttattttt ttgagctttg atctttcttt aaactgatct attttttaat
     3181 tgattggtta tggtgtaaat attacatagc tttaactgat aatctgatta ctttatttcg
     3241 tgtgtctatg atgatgatga tagttacaga accgacgact cgtccgtcct gtagaaaccc
     3301 caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact
     3361 gtggaattga tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc
     3421 caggcagttt taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct
     3481 ggtatcagcg cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt
     3541 tcgatgcggt cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc
     3601 agggcggcta tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg
     3661 tacgtatcac cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg
     3721 tgattaccga cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg
     3781 ccggaatcca tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca
     3841 ccgtggtgac gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg
     3901 ccaatggtga tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac
     3961 aaggcactag cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt
     4021 atctctatga actgtgcgtc acagccaaaa gccagacaga gtgtgatatc tacccgcttc
     4081 gcgtcggcat ccggtcagtg gcagtgaagg gcgaacagtt cctgattaac cacaaaccgt
     4141 tctactttac tggctttggt cgtcatgaag atgcggactt gcgtggcaaa ggattcgata
     4201 acgtgctgat ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta
     4261 cctcgcatta cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg
     4321 tgattgatga aactgctgct gtcggcttta acctctcttt aggcattggt ttcgaagcgg
     4381 gcaacaagcc gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc
     4441 acttacaggc gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt
     4501 ggagtattgc caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac
     4561 tggcggaagc aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt
     4621 tctgcgacgc tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt
     4681 attacggatg gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag
     4741 aacttctggc ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg
     4801 atacgttagc cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg
     4861 catggctgga tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg
     4921 tatggaattt cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga
     4981 aagggatctt cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct
     5041 ggactggcat gaacttcggt gaaaaaccgc agcagggagg caaacaagct agccaccacc
     5101 accaccacca cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg ttcaaacatt
     5161 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     5221 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     5281 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     5341 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     5401 gaattaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     5461 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     5521 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     5581 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     5641 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     5701 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     5761 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     5821 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     5881 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     5941 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     6001 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     6061 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     6121 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     6181 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     6241 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     6301 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     6361 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     6421 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     6481 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     6541 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     6601 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     6661 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     6721 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     6781 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     6841 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     6901 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     6961 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     7021 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     7081 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     7141 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     7201 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     7261 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     7321 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     7381 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     7441 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     7501 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     7561 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     7621 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     7681 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     7741 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     7801 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     7861 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     7921 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     7981 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     8041 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     8101 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     8161 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     8221 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     8281 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     8341 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     8401 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     8461 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     8521 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     8581 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     8641 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     8701 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     8761 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     8821 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     8881 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
     8941 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     9001 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     9061 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     9121 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     9181 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     9241 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     9301 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
     9361 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     9421 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     9481 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     9541 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     9601 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9661 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9721 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9781 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9901 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9961 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10021 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10081 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10141 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10201 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    10261 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10321 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10381 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10441 aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta
    10501 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
    10561 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
    10621 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
    10681 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
    10741 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
    10801 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
    10861 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
    10921 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
    10981 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
    11041 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
    11101 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
    11161 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
    11221 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
    11281 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
    11341 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
    11401 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg
    11461 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
    11521 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
    11581 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
    11641 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
    11701 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
    11761 acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg
    11821 gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca
    11881 tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc
    11941 ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga tcgacagatc
    12001 ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt cggtttccac
    12061 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
    12121 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
    12181 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
    12241 ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt
    12301 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
    12361 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
    12421 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
    12481 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
    12541 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
    12601 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
    12661 taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga acagcgggca
    12721 gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag atgcaatagg
    12781 tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
    12841 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
    12901 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
    12961 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
    13021 cggtgagttc aggctttttc atatctcatt gccccccgga tctgcgaaag ctcgagagag
    13081 atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc
    13141 cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
    13201 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
    13261 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
    13321 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
    13381 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
    13441 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
    13501 tggagtagac gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa
    13561 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    13621 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    13681 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    13741 atttcacaca ggaaacagct atgacatgat tacgaattcg agctcggtac ccggggatcc
    13801 tctagctaga ttaattaagt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    13861 ggagatcctt tggatcacaa aaaaaaagcc cgctcattag gcgggttaaa ttcccgggta
    13921 ctgcagccga ttcattaatg caggttaacc tggcttatcg aaattaatac gactcactat
    13981 agggagaccg gcagatctga tccctactcc aaaaatgtca aagatacagt ctcagaagac
    14041 caaagggcta ttgagacttt tcaacaaagg gtaatttcgg gaaacctcct cggattccat
    14101 tgcccagcta tctgtcactt catcgaaagg acagtggaaa aggaaggtgg ctcctacaaa
    14161 tgccatcatt gcgataaagg aaaggctatc attcaagatg cctctgccga cagtggtccc
    14221 aaagatggac ccccacccac gaggggcatc gtgggaaaag aagacgttcc aaccacgtct
    14281 tcaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc acaatcccac
    14341 ccctactcca aaaatgtcaa agatactgtc tcagaagacc aaagggctat
//
