LOCUS       sequence-174338-       14464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disturb the expression of miR1432 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14464)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM1432
FEATURES             Location/Qualifiers
     source          1..14464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    527..703
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1265..1283)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1266..1283)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(1354..2013)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKEITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1928..1947
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2050..2055,2056..2072,2073..2078))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2337..2354)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2337..2353)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2346..2368)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2730..3075
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3092..3106,3297..5114)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          3107..3296
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             5121..5138
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5173..5425
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5447..5471
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6772..7401
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7830..8903
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8969..9163
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9399..9421)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9507..9647
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9562..9581
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9662..9679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9833..10421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10508..11302)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11727..11751
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11829..12003
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12044..13069)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(13142..13487)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    13678..13699
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13714..13719,13720..13737,13738..13744)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13752..13768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13757..13779
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13776..13792
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13776..13792
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14039..14058
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14039..14057
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctac
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcatcg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttggta
      361 cctgtcggtg tcattcactc tcctgatgtt gttgttgtta tggtctaatt taaatatggt
      421 ctaaagaaga agaattgtcg gtgtcattca ctctcctgat ggatccacta gtgaattcgg
      481 tacgctgaaa tcaccagtct ctctctacaa atctatctct ctctattttc tccataaata
      541 atgtgtgagt agtttcccga taagggaaat tagggttctt atagggtttc gctcatgtgt
      601 tgagcatata agagaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
      661 tttctaattc ctaaaaccaa aatccagtac taaaatccag atctcctaaa gtccctatag
      721 atctttgtcg tgaatataaa ccagacacga gacgactaga cctggagccc agacgccgtt
      781 cgaagctaga agtaccgctt aggcaggagg ccgttaggga aaagatgcta aggcagggtt
      841 ggttacgttg actcccccgt aggtttggtt taaatatgat gaagtggacg gaaggaagga
      901 ggaagacaag gaaggataag gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat
      961 agaggtacgc tactatactt atactatacg ctaagggaat gattgtattt ataccctata
     1021 ccccctaata accccttatc aatttaagaa ataatccgca taagcccccg cttaaaaatt
     1081 ggtatcagag ccatgaatag gtctatgacc aaaactcaag aggataaaac ctcaccaaaa
     1141 tacgaaagag tccttaactc taaagataaa agatctttca agatcaaaac tagttccctc
     1201 acaccggtga cggggatcgc atgcgacctc tagaccatca cattggagct tcagctgctc
     1261 gagttctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
     1321 agccgcgttc taacgtcgac aggcctgtac caattacgcc ccgccctgcc actcatcgca
     1381 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     1441 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     1501 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1561 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     1621 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1681 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1741 acaagggtga acactatccc atattaccag ctcaccgtct ttcattgcca tacggaactc
     1801 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1861 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1921 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1981 aacggtggta tatccagtga tttctttctc cattttagct tccttagctc ctgaaaatct
     2041 cgataactca ttaaaactta tcgatgataa gctgtcaaac atgagaattt agcccgccta
     2101 atgagcgggc tttttgctgt gatccgcgtt gctggcgttt ttccataggc tccgcccccc
     2161 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2221 aagataccag gcgtttcccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2281 cgcttaccgg atacctgtcc gcctttctta attaactgca ggcatgcaag cttggcactg
     2341 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     2401 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     2461 tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt
     2521 gtcgtttccc gccttcagtt tagcttcatg gagtcaaaga ttcaaataga ggacctaaca
     2581 gaactcgccg taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag
     2641 aagaaaatct tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa
     2701 gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga
     2761 aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag
     2821 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc
     2881 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     2941 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
     3001 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     3061 catttggaga gaacacgggg gactcttgac catggtagat ctgagggtaa atttctagtt
     3121 tttctccttc attttcttgg ttaggaccct tttctctttt tatttttttg agctttgatc
     3181 tttctttaaa ctgatctatt ttttaattga ttggttatgg tgtaaatatt acatagcttt
     3241 aactgataat ctgattactt tatttcgtgt gtctatgatg atgatgatag ttacagaacc
     3301 gacgactcgt ccgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt
     3361 gggcattcag tctggatcgc gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt
     3421 tacaagaaag ccgggcaatt gctgtgccag gcagttttaa cgatcagttc gccgatgcag
     3481 atattcgtaa ttatgcgggc aacgtctggt atcagcgcga agtctttata ccgaaaggtt
     3541 gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg
     3601 tcaataatca ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca
     3661 cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga
     3721 actggcagac tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt
     3781 cttacttcca tgatttcttt aactatgccg gaatccatcg cagcgtaatg ctctacacca
     3841 cgccgaacac ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc
     3901 acgcgtctgt tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg
     3961 cggatcaaca ggtggttgca actggacaag gcactagcgg gactttgcaa gtggtgaatc
     4021 cgcacctctg gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc
     4081 agacagagtg tgatatctac ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg
     4141 aacagttcct gattaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg
     4201 cggacttgcg tggcaaagga ttcgataacg tgctgatggt gcacgaccac gcattaatgg
     4261 actggattgg ggccaactcc taccgtacct cgcattaccc ttacgctgaa gagatgctcg
     4321 actgggcaga tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc
     4381 tctctttagg cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg
     4441 cagtcaacgg ggaaactcag caagcgcact tacaggcgat taaagagctg atagcgcgtg
     4501 acaaaaacca cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc
     4561 aaggtgcacg ggaatatttc gcgccactgg cggaagcaac gcgtaaactc gacccgacgc
     4621 gtccgatcac ctgcgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc
     4681 tctttgatgt gctgtgcctg aaccgttatt acggatggta tgtccaaagc ggcgatttgg
     4741 aaacggcaga gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc
     4801 cgattatcat caccgaatac ggcgtggata cgttagccgg gctgcactca atgtacaccg
     4861 acatgtggag tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc
     4921 gcgtcagcgc cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg acctcgcaag
     4981 gcatattgcg cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt
     5041 cggcggcttt tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc
     5101 agggaggcaa acaagctagc caccaccacc accaccacgt gtgaattaca ggtgaccagc
     5161 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     5221 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     5281 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     5341 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     5401 gcggtgtcat ctatgttact agatcgggaa ttaaactatc agtgtttgac aggatatatt
     5461 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
     5521 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
     5581 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     5641 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     5701 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     5761 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     5821 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     5881 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     5941 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     6001 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     6061 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     6121 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     6181 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
     6241 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     6301 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     6361 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     6421 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     6481 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     6541 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
     6601 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     6661 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     6721 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     6781 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     6841 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     6901 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     6961 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     7021 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     7081 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     7141 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     7201 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     7261 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     7321 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     7381 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     7441 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     7501 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     7561 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     7621 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     7681 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     7741 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc
     7801 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
     7861 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
     7921 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     7981 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     8041 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     8101 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     8161 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     8221 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     8281 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     8341 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     8401 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     8461 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     8521 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     8581 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     8641 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     8701 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     8761 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     8821 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     8881 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     8941 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
     9001 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
     9061 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
     9121 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
     9181 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
     9241 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
     9301 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
     9361 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     9421 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9481 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     9541 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     9601 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     9661 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9721 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9781 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9841 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9901 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9961 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10021 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10081 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10141 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10201 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10261 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10321 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10381 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10441 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt
    10501 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
    10561 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
    10621 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
    10681 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
    10741 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
    10801 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
    10861 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
    10921 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
    10981 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
    11041 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
    11101 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
    11161 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
    11221 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
    11281 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
    11341 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
    11401 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
    11461 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta
    11521 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
    11581 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
    11641 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
    11701 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
    11761 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta
    11821 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga
    11881 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag
    11941 cgaaacccta taggaaccct aattccctta tctgggaact actcacacat tattatggag
    12001 aaactcgagc ttgtcgatcg acagatcccg gtcggcatct actctatttc tttgccctcg
    12061 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
    12121 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
    12181 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
    12241 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg
    12301 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
    12361 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
    12421 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
    12481 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
    12541 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
    12601 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
    12661 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct
    12721 ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
    12781 cctgtgaacg gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca
    12841 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
    12901 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
    12961 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
    13021 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
    13081 ccccggatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc
    13141 gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
    13201 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
    13261 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
    13321 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
    13381 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
    13441 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
    13501 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
    13561 tggcaagctg ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    13621 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    13681 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    13741 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac
    13801 gaattcgagc tcggtacccg gggatcctct agctagatta attaagtttg caagcagcag
    13861 attaccggat acctgtccgc ctttcttaat taagtttgca agcagcagat tacgcgcaga
    13921 aaaaaaggat ctcaaggaga tcctttggat cacaaaaaaa agcccgctca ttaggcgggt
    13981 taaattcccg ggtactgcag ccgattcatt aatgcaggtt aacctggctt atcgaaatta
    14041 atacgactca ctatagggag accggtagat ctgatcccta ctccaaaaat gtcaaagata
    14101 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
    14161 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagtg gaaaaggaag
    14221 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
    14281 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggga aaagaagacg
    14341 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
    14401 acgcacaatc ccgcccctac tccaaaaatg tcaaagatac tgtctcagaa gaccaaaggg
    14461 ctat
//
