LOCUS       sequence-174340-       14722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disturb the expression of miR827 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14722)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM827
FEATURES             Location/Qualifiers
     source          1..14722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    513..689
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1251..1269)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1252..1269)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(join(2030..2035,2036..2052,2053..2058))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2341..2357)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2341..2357)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2354..2376)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2365..2381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2389..2395,2396..2413,2414..2419))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2434..2455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2646..3322
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3389..4414
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4455..4629
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4707..4731
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5156..5950
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6037..6625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6779..6796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6811..6951
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6877..6896)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7037..7059
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7295..7489
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7555..8628)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9057..9686)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10987..11011
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11033..11285)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11320..11337)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(11344..13161,13352..13366))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          13162..13351
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(13383..13728)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     14090..14112
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14104..14121
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14105..14121
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14297..14316
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14297..14315
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt cagagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagctttgtt
      361 tgctgatcta ggtcatctaa gttgttgttg ttatggtcta atttaaatgg aaatatggtc
      421 taaagaagaa gaattgtttg ctgatctagg tcatctaaaa attcggtacg ctgaaatcac
      481 cagtctctct ctacaaatct atctctctct attttctcca taaataatgt gtgagtagtt
      541 tcccgataag ggaaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa
      601 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
      661 aaccaaaatc cagtactaaa atccagatct cctaaagtcc ctatagatct ttgtcgtgaa
      721 tataaaccag acacgagacg actagacctg gagcccagac gccgttcgaa gctagaagta
      781 ccgcttaggc aggaggccgt tagggaaaag atgctaaggc agggttggtt acgttgactc
      841 ccccgtaggt ttggtttaaa tatgatgaag tggacggaag gaaggaggaa gacaaggaag
      901 gataaggttg caggccctgt gcaaggtaag aagatggaaa tttgatagag gtacgctact
      961 atacttatac tatacgctaa gggaatgatt gtatttatac cctatacccc ctaataaccc
     1021 cttatcaatt taagaaataa tccgcataag cccccgctta aaaattggta tcagagccat
     1081 gaataggtct atgaccaaaa ctcaagagga taaaacctca ccaaaatacg aaagagtcct
     1141 taactctaaa gataaaagat ctttcaagat caaaactagt tccctcacac cggtgacggg
     1201 gatcgcatgc gacctctaga ccatcacatt ggagcttcag ctgctcgagt tctatagtgt
     1261 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttcttac
     1321 gtcgacgtac caattacgcc ccgccctgtc actcatcgca gtactgttgt aattcattaa
     1381 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     1441 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1501 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     1561 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1621 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1681 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1741 atattaccag ctcaccgtct ttcattgcca tacggaactc cggatgagca ttcatcaggc
     1801 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1861 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgga
     1921 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tacccagtga
     1981 tttctttctc cattttagct tccttagctc ctgaaaatct cgataactca ttaaagctta
     2041 tcgatgataa gctgtcaaac atgagaattt agcccgccta atgagcgggc tttttgttgt
     2101 gatccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2161 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2221 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2281 gcctttctta attaatctag ctagaggatc cccgggtacc gagctcgaat tcgtaatcat
     2341 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2401 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2461 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2521 cggccaacgc gcggggagag gcggtttgcg tattggctag agcagcttgc caacatggtg
     2581 gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg
     2641 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
     2701 gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat
     2761 cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat
     2821 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     2881 caagtggatt gatgtgaaca tggtggagca cgacactctc gtctactcca agaatatcaa
     2941 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
     3001 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
     3061 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     3121 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     3181 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     3241 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     3301 tcatttggag aggacacgct gaaatcacca gtctctctct acaaatctat ctctctcgag
     3361 ctttcgcaga tccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt
     3421 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     3481 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     3541 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     3601 gctcccgatt ccggaagtgc ttgacattgg ggagtttagc gagagcctga cctattgcat
     3661 ctcccgccgt tcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     3721 tctacaaccg gtcgcggagg ctatggatgc gatcgctgcg gccgatctta gccagacgag
     3781 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     3841 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     3901 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     3961 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     4021 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     4081 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     4141 gaggcatccg gagcttgcag gatcgccacg actccgggcg tatatgctcc gcattggtct
     4201 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     4261 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     4321 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     4381 acgccccagc actcgtccga gggcaaagaa atagagtaga tgccgaccgg gatctgtcga
     4441 tcgacaagct cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg
     4501 gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
     4561 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa
     4621 atccagatcc cccgaattaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg
     4681 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc
     4741 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca
     4801 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
     4861 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
     4921 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca
     4981 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     5041 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     5101 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     5161 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     5221 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     5281 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     5341 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     5401 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     5461 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     5521 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     5581 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     5641 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     5701 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     5761 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     5821 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     5881 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     5941 attgttttag tacctagaat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6181 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6661 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6721 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6781 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     6841 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     6901 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     6961 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7021 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7081 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     7141 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     7201 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     7261 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     7321 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     7381 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     7441 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     7501 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     7561 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     7621 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     7681 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     7741 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     7801 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     7861 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     7921 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     7981 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     8041 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     8101 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     8161 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     8221 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     8281 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     8341 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     8401 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     8461 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     8521 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     8581 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     8641 ggcttggggg ttccagtgcc attgcagggc cggcaggcaa cccagccgct tacgcctggc
     8701 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     8761 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     8821 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     8881 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     8941 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     9001 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
     9061 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
     9121 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
     9181 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
     9241 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
     9301 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
     9361 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
     9421 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
     9481 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
     9541 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
     9601 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
     9661 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
     9721 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
     9781 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
     9841 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
     9901 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
     9961 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    10021 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    10081 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    10141 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    10201 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    10261 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    10321 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    10381 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    10441 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    10501 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    10561 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    10621 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    10681 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    10741 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    10801 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    10861 gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg
    10921 aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc
    10981 tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaattc ccgatctagt
    11041 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
    11101 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
    11161 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
    11221 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
    11281 cgatcgggga aattcgagct ggtcacctgt aattcacacg tggtggtggt ggtggtggct
    11341 agcttgtttg cctccctgct gcggtttttc accgaagttc atgccagtcc agcgtttttg
    11401 cagcagaaaa gccgccgact tcggtttgcg gtcgcgagtg aagatccctt tcttgttacc
    11461 gccaacgcgc aatatgcctt gcgaggtcgc aaaatcggcg aaattccata cctgttcacc
    11521 gacgacggcg ctgacgcgat caaagacgcg gtgatacata tccagccatg cacactgata
    11581 ctcttcactc cacatgtcgg tgtacattga gtgcagcccg gctaacgtat ccacgccgta
    11641 ttcggtgatg ataatcggct gatgcagttt ctcctgccag gccagaagtt ctttttccag
    11701 taccttctct gccgtttcca aatcgccgct ttggacatac catccgtaat aacggttcag
    11761 gcacagcaca tcaaagagat cgctgatggt atcggtgtga gcgtcgcaga acattacatt
    11821 gacgcaggtg atcggacgcg tcgggtcgag tttacgcgtt gcttccgcca gtggcgcgaa
    11881 atattcccgt gcaccttgcg gacgggtatc cggttcgttg gcaatactcc acatcaccac
    11941 gcttgggtgg tttttgtcac gcgctatcag ctctttaatc gcctgtaagt gcgcttgctg
    12001 agtttccccg ttgactgcct cttcgctgta cagttctttc ggcttgttgc ccgcttcgaa
    12061 accaatgcct aaagagaggt taaagccgac agcagcagtt tcatcaatca ccacgatgcc
    12121 atgttcatct gcccagtcga gcatctcttc agcgtaaggg taatgcgagg tacggtagga
    12181 gttggcccca atccagtcca ttaatgcgtg gtcgtgcacc atcagcacgt tatcgaatcc
    12241 tttgccacgc aagtccgcat cttcatgacg accaaagcca gtaaagtaga acggtttgtg
    12301 gttaatcagg aactgttcgc ccttcactgc cactgaccgg atgccgacgc gaagcgggta
    12361 gatatcacac tctgtctggc ttttggctgt gacgcacagt tcatagagat aaccttcacc
    12421 cggttgccag aggtgcggat tcaccacttg caaagtcccg ctagtgcctt gtccagttgc
    12481 aaccacctgt tgatccgcat cacgcagttc aacgctgaca tcaccattgg ccaccacctg
    12541 ccagtcaaca gacgcgtggt tacagtcttg cgcgacatgc gtcaccacgg tgatatcgtc
    12601 cacccaggtg ttcggcgtgg tgtagagcat tacgctgcga tggattccgg catagttaaa
    12661 gaaatcatgg aagtaagact gctttttctt gccgttttcg tcggtaatca ccattcccgg
    12721 cgggatagtc tgccagttca gttcgttgtt cacacaaacg gtgatacgta cacttttccc
    12781 ggcaataaca tacggcgtga catcggcttc aaatggcgta tagccgccct gatgctccat
    12841 cacttcctga ttattgaccc acactttgcc gtaatgagtg accgcatcga aacgcagcac
    12901 gatacgctgg cctgcccaac ctttcggtat aaagacttcg cgctgatacc agacgttgcc
    12961 cgcataatta cgaatatctg catcggcgaa ctgatcgtta aaactgcctg gcacagcaat
    13021 tgcccggctt tcttgtaacg cgctttccca ccaacgctga tcaattccac agttttcgcg
    13081 atccagactg aatgcccaca ggccgtcgag ttttttgatt tcacgggttg gggtttctac
    13141 aggacggacg agtcgtcggt tctgtaacta tcatcatcat catagacaca cgaaataaag
    13201 taatcagatt atcagttaaa gctatgtaat atttacacca taaccaatca attaaaaaat
    13261 agatcagttt aaagaaagat caaagctcaa aaaaataaaa agagaaaagg gtcctaacca
    13321 agaaaatgaa ggagaaaaac tagaaattta ccctcagatc taccatggtc aagagtcccc
    13381 cgtgttctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag
    13441 tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa
    13501 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
    13561 ggaccactgt cggcagaggc atcttcaacg atggcctttc ctttatcgca atgatggcat
    13621 ttgtaggagc caccttcctt ttccactatc ttcacaataa agtgacagat agctgggcaa
    13681 tggaatccga ggaggtttcc ggatattacc ctttgttgaa aagtctcaat tgccctttgg
    13741 tcttctgaga ctgtatcttt gatatttttg gagtagacaa gtgtgtcgtg ctccaccatg
    13801 ttgacgaaga ttttcttctt gtcattgagt cgtaagagac tctgtatgaa ctgttcgcca
    13861 gtctttacgg cgagttctgt taggtcctct atttgaatct ttgactccat gaagctaaac
    13921 tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagcattc gccattcagg
    13981 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    14041 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    14101 cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc agttaattaa gtttgcaagc
    14161 agcagattac gcgcagaaaa aaaggatctc aaggagatcc tttggatcac aaaaaaaaag
    14221 cccgctcatt aggcgggtta aattcccggg tactgcagcc gattcattaa tgcaggttaa
    14281 cctggcttat cgaaattaat acgactcact atagggagac cggcagatct gatccctact
    14341 ccaaaaatgt caaagataca gtctcagaag accaaagggc tattgagact tttcaacaag
    14401 gggtaatttc gggaaacctc ctcgggttcc attgcccagc tatctgtcac ttcatcgaaa
    14461 ggacagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta
    14521 tcattcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
    14581 tcgtgggaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgacatct
    14641 ccactgacgt aagggatgac gcacaatccc acccctactc caaaaatgtc aaagatactg
    14701 tctcagaaga ccaaagggct at
//
