LOCUS       sequence-174343-        6072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hybrid Tar[1-43]-McpC[49-270]-Tar[184-553] expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Bi S, Pollard AM, Yang Y, Jin F, Sourjik V
  TITLE     Engineering Hybrid Chemotaxis Receptors in Bacteria.
  JOURNAL   ACS Synth Biol. 2016 Jun 10.
  PUBMED    27285081
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jun 10."
COMMENT     Sequence Label: pSB1
FEATURES             Location/Qualifiers
     source          1..6072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      403..437
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(856..1515)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1430..1449
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1516..1618)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(2010..2027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2144..2689)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2224..2243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atatcgtcaa tgctgtcact cgcgtgactg acattatggg cgagattgca tcggcatcgg
       61 atgaacagag ccgtggcatc gatcaagtcg cattggcggt ttcggaaatg gatcgcgtca
      121 cgcaacagaa cgcatcgctg gtgcaggaat cagctgccgc cgccgctgcg ctggaagaac
      181 aggcgagtcg tttaacgcaa gcagtttccg cgttccgtct ggcagccagc ccactcacca
      241 ataaaccgca aacaccatcc cgtcctgcca gtgagcaacc accggctcag ccacgactgc
      301 gaattgctga acaagatcca aactgggaaa cattttgagg atccgcggat aaataagtaa
      361 cgatccggtc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc
      421 cgccgggcgt tttttattgg tgagaatcgc agcaacttgt cgcgccaatc gagccatgtc
      481 gtcgtcaacg accccccatt caagaacagc aagcagcatt gagaactttg gaatccagtc
      541 cctcttccac ctgctgaccg gatcagcagt ccccggaaca tcgtagctga cgccttcgcg
      601 ttgctcagtt gtccaacccc ggaaacggga aaaagcaagt tttccccgct cccggcgttt
      661 caataactga aaaccatact atttcacagt ttaaatcaca ttaaacgaca gtaatccccg
      721 ttgatttgtg cgccaacaca gatccgtcga ccgggtcgaa tttgctttcg aatttctgcc
      781 attcatccgc ttattatcac ttattcaggc gtagcaacca ggcgtttaag ggcaccaata
      841 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
      901 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
      961 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1021 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     1081 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     1141 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     1201 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     1261 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag
     1321 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     1381 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     1441 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     1501 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     1561 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac
     1621 gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt
     1681 tatttattct gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg
     1741 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
     1801 ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
     1861 gcaccgccgg acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga
     1921 gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag
     1981 aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt
     2041 cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg
     2101 aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc
     2161 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     2221 tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct
     2281 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca
     2341 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
     2401 tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca
     2461 aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg
     2521 cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt
     2581 acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt
     2641 tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct
     2701 tattaatcag ataaaatatt tctagatttc agtgcaattt atctcttcaa atgtagcacc
     2761 tgaagtcagc cccatacgat ataagttgta attctcatgt ttgacagctt atcatcgatc
     2821 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac
     2881 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta
     2941 gcaatttaac tgtgataaac taccgcatta aagcttcttg cgaaacagct ctcgaccagg
     3001 tttatactgg tcgtgcagat gaaagcagtt tttgccgagg tcgattccga ccagcgccat
     3061 atcgctcata gtgatggtct ccgaattata aacaccctgc gaaagcgtag cctacgcagg
     3121 gtgtcgggtg ctcatcccat tacccgtttt cccatctctt aacttaaggg gggaacggtg
     3181 tcctgtcttt catggggctc gttcactgct aacttacctg cttccctgtc tgtcaattct
     3241 ctctatcctg cgatcccgcg aagaaccaaa aaagctcgac agagggcgcg gtcattttag
     3301 gtcgggcgga tcggcgccgc cggctcggct ggtgtgccgc acagcaccgc ctacgtgagc
     3361 tgccagttga tgaacttccc ccgttgccag ctagggcgca agcgggctgt ataagatcac
     3421 tgcccatcac attgatcggc tcggattttt tctcaatccg taaacaggtc aaacatcagt
     3481 tgccgcaacc aaatattggc taggtccttg tggtacttcg catgccagaa catgttgatg
     3541 gctatttcag gcaagacgac tgggtgcggc aaggcgctta ggccgaaggg ctccacacag
     3601 cagtcggcta aacgtatcgg cacagtggcg agcagatcgg tgcgctggag gatgtggcca
     3661 acggcggcga agtgcggcac ttccagacgg atgtcgcgcc ggatgccgac ccgtgtcatg
     3721 tacgtgtcca cctcgccgtg gccggtgcca gcggcgatga cacgcacgtg gccgtaggaa
     3781 cagaagcgct ccagagtcag gggttcgcgg gtgactggat ggtccttgcg acataggcac
     3841 acgtagtgat tctggagcag ccggcgctga aagaagccag tttgcagatt gggaagcagg
     3901 cccacggcca agtccacggt tccgttctgc aaggcctgca tcaggctcat cgaactgtcg
     3961 cgcaccgtac tgatcacgca attgggggcc tggtgagcca gcacatccat cagccgcggc
     4021 atgaagtaga tctcgccaat gtcggtcatg gccagggtga aggtacgctc gctggtcagc
     4081 ggatcgaagc tttcatggtg ctgtagggcg ttgcgcagtg cgtgcatggc cgaagtgacg
     4141 ggctcggcca gatgcgcggc atagggtgtg ggttccattc cctgatgtgt gcgcacgaag
     4201 agtgggtcct gtagcgaggt gcgcaggcgt ttcagcgcat tgctcacggc aggctgggtc
     4261 aggcccaggt tctccgcagt gatagagacg cgtctgtcga ccagcaactg gttgaacacc
     4321 accagcaggt ttaaatccag gtcacgcagt tccatggggc ctcgcttggg ttattgctgg
     4381 tgcccggccg ggcgcaatat tcatgttgat gatttattat atatcgagtg gtgtatttat
     4441 caatattgtt tgctccgtta tcgttattaa caagtcatca ataaagccat aaggagtacc
     4501 atatgattaa ccgtatccgc gtagtcacgc tgttggtaat ggtgctgggg gtattcgcac
     4561 tgttacagct tatttccggc agtctgtttt tttcttccct tcaccatagc cagaagagct
     4621 ttgtggtttc ccagtcactt gaacaaaata ttgaattgca attaaagagt tatgccattt
     4681 cgttatcaag attggctaat ggggaattaa cgcatacatt tgtaacgaaa ccaagtaaag
     4741 aagcttctcg gctgtttcac gatgacatta agcaaatcaa agacaacgat gattatgttg
     4801 cgatggctta cataggcacc gcaaaaaaag aaatgtttac atatccaaaa gcggatttcg
     4861 ctgaggatta cgatccaaca tcaagaccat ggtataagct tgcagctgaa accccggatc
     4921 aagttgtttg gacggaacct tataaagatg tcgtgacagg agacatgatt gttactgcat
     4981 cgaaagcgat cttagacagg caaaaagtga taggggttgc cagctatgac ttgaaactct
     5041 ctgctatcca gagtatggtc aataaacaaa aggtgcctta caaaggattt gcctttttag
     5101 ctgatgcgag cggcaatctt ttagcccatc cgtcaaatca aggaaaaaat atatcaaagg
     5161 atcaaacact gcaaaccatc gcatccgaga aaaaagggat acaagatgtg aatgggaaaa
     5221 tggttgtgta ccaaacgatt ggcgagacag gctggaaggt cggaacgcaa tttgataccg
     5281 atcaattaat gtggattgca gatgattacc gatttgccca gtggcaactg gcggttatcg
     5341 cgctggtggt ggtattgatt ctgctggtgg cgtggtacgg cattcgccgt atgttgctta
     5401 ctccgctggc aaaaattatt gctcacattc gcgaaatcgc cggtggtaac ctggcgaata
     5461 ccctgaccat tgacgggcgc agtgaaatgg gcgacctggc gcagagcgtt tcacacatgc
     5521 aacgctcttt gactgacacc gtcactcatg tccgcgaagg ttcagatgcc atctatgccg
     5581 gtacccgtga aattgcggcg ggcaacaccg atctttcctc ccgtactgaa cagcaggcat
     5641 ccgcgctgga agaaattgcc gccagcatgg agcagctcac cgcgacagtg aagcaaaacg
     5701 ccgataacgc ccgccaggcc tcgcaactgg cgcaaagtgc ctccgacacc gcccagcacg
     5761 gcggcaaagt ggtggatggc gtagtgaaaa cgatgcatga gatcgccgat agttcgaaga
     5821 aaattgccga cattatcagc gttatcgacg gtattgcctt ccagactaat atcctcgcgc
     5881 tgaatgccgc ggttgaagcc gcgcgtgcgg gtgaacaggg ccgtggtttt gccgtggtgg
     5941 cgggtgaagt gcgtaatctt gccagtcgca gcgcccaggc ggcaaaagag atcaaagccc
     6001 tcattgaaga ctccgtctca cgcgttgata ccggttcggt gctggtcgaa agcgccgggg
     6061 aaacaatgaa ca
//
