LOCUS       sequence-174345-       14714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disturb the expression of miR530-3p in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14714)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM530-3p
FEATURES             Location/Qualifiers
     source          1..14714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2333..2349)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2333..2349)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2346..2368)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2357..2373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2381..2387,2388..2405,2406..2411))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2426..2447
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2638..3314
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3381..4406
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4447..4621
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4699..4723
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5148..5942
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6029..6617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6771..6788
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6803..6943
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6869..6888)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7029..7051
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7287..7481
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7547..8620)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9049..9678)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10979..11003
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11025..11277)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11312..11329)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(11336..13153,13344..13358))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          13154..13343
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(13375..13720)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     14082..14104
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14096..14113
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14097..14113
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14289..14308
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14289..14307
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacgtctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttgttg
      361 catctgccta ctctgcacct gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatgt tgcatctgcc cactctgcac ctgaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taaggaaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc taggccatca cattggagct tcagctgctc gagttctata gtgtcaccca
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtc ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttataatct agctagagga tccccgggta ccgagctcga attcgtaatc atgtcatagc
     2341 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2401 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2461 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2521 gcgcggggag aggcggtttg cgtattggct agagcagctt gccaacatgg tggagcacga
     2581 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
     2641 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
     2701 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
     2761 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     2821 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     2881 ttgatgtgaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
     2941 tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
     3001 tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg
     3061 gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg
     3121 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
     3181 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
     3241 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
     3301 agaggacacg ctgaaatcac cagtctctct ctacaaatct atctctctcg agctttcgca
     3361 gatccggggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     3421 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     3481 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     3541 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     3601 ttccggaagt gcttgacatt ggggagttta gcgagagcct gacctattgc atctcccgcc
     3661 gttcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctacaac
     3721 cggtcgcgga ggctatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     3781 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     3841 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     3901 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     3961 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     4021 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     4081 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     4141 cggagcttgc aggatcgcca cgactccggg cgtatatgct ccgcattggt cttgaccaac
     4201 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     4261 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     4321 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     4381 gcactcgtcc gagggcaaag aaatagagta gatgccgacc gggatctgtc gatcgacaag
     4441 ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat
     4501 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     4561 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat
     4621 cccccgaatt aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
     4681 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     4741 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     4801 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     4861 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     4921 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc
     4981 tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg
     5041 tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct
     5101 tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga
     5161 gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag
     5221 gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa
     5281 tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc
     5341 tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct
     5401 ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg
     5461 aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca
     5521 tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt
     5581 acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat
     5641 ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg
     5701 tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg
     5761 gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg
     5821 tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac
     5881 tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt
     5941 agtacctaga atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6001 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6061 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6121 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     6181 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6241 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6301 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6361 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6421 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6481 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6541 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6601 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6661 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6721 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6781 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     6841 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     6901 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     6961 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     7021 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     7081 gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc
     7141 ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc
     7201 gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct
     7261 cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa
     7321 taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt
     7381 acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc
     7441 caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac
     7501 ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg
     7561 atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg
     7621 cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga
     7681 aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc
     7741 atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc
     7801 cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg
     7861 tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt
     7921 cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc
     7981 ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc
     8041 aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta
     8101 cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc
     8161 ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac
     8221 tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga
     8281 tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc
     8341 tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt
     8401 cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt
     8461 tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc
     8521 gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg
     8581 cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg
     8641 ggttccagtg ccattgcagg gccggcaggc aacccagccg cttacgcctg gccaaccgcc
     8701 cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat
     8761 gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg
     8821 gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt
     8881 acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg
     8941 gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg
     9001 cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt
     9061 tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga
     9121 ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg
     9181 ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg
     9241 cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc
     9301 aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg
     9361 ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc
     9421 ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc
     9481 aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca
     9541 atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg
     9601 cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag
     9661 tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata
     9721 tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg
     9781 cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac
     9841 aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg
     9901 tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg
     9961 ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg
    10021 cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg
    10081 tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg
    10141 cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct
    10201 gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc
    10261 gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca
    10321 cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac
    10381 gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc
    10441 gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca
    10501 tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg
    10561 tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga
    10621 cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt
    10681 tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa
    10741 agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag
    10801 acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag
    10861 tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata
    10921 aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt
    10981 ttacccgcca atatatcctg tcaaacactg atagtttaat tcccgatcta gtaacataga
    11041 tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta
    11101 ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca
    11161 ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcatcgc
    11221 aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg
    11281 gaaattcgag ctggtcacct gtaattcaca cgtggtggtg gtggtggtgg ctagcttgtt
    11341 tgcctccctg ctgcggtttt tcaccgaagt tcatgccagt ccagcgtttt tgcagcagaa
    11401 aagccgccga cttcggtttg cggtcgcgag tgaagatccc tttcttgtta ccgccaacgc
    11461 gcaatatgcc ttgcgaggtc gcaaaatcgg cgaaattcca tacctgttca ccgacgacgg
    11521 cgctgacgcg atcaaagacg cggtgataca tatccagcca tgcacactga tactcttcac
    11581 tccacatgtc ggtgtacatt gagtgcagcc cggctaacgt atccacgccg tattcggtga
    11641 tgataatcgg ctgatgcagt ttctcctgcc aggccagaag ttctttttcc agtaccttct
    11701 ctgccgtttc caaatcgccg ctttggacat accatccgta ataacggttc aggcacagca
    11761 catcaaagag atcgctgatg gtatcggtgt gagcgtcgca gaacattaca ttgacgcagg
    11821 tgatcggacg cgtcgggtcg agtttacgcg ttgcttccgc cagtggcgcg aaatattccc
    11881 gtgcaccttg cggacgggta tccggttcgt tggcaatact ccacatcacc acgcttgggt
    11941 ggtttttgtc acgcgctatc agctctttaa tcgcctgtaa gtgcgcttgc tgagtttccc
    12001 cgttgactgc ctcttcgctg tacagttctt tcggcttgtt gcccgcttcg aaaccaatgc
    12061 ctaaagagag gttaaagccg acagcagcag tttcatcaat caccacgatg ccatgttcat
    12121 ctgcccagtc gagcatctct tcagcgtaag ggtaatgcga ggtacggtag gagttggccc
    12181 caatccagtc cattaatgcg tggtcgtgca ccatcagcac gttatcgaat cctttgccac
    12241 gcaagtccgc atcttcatga cgaccaaagc cagtaaagta gaacggtttg tggttaatca
    12301 ggaactgttc gcccttcact gccactgacc ggatgccgac gcgaagcggg tagatatcac
    12361 actctgtctg gcttttggct gtgacgcaca gttcatagag ataaccttca cccggttgcc
    12421 agaggtgcgg attcaccact tgcaaagtcc cgctagtgcc ttgtccagtt gcaaccacct
    12481 gttgatccgc atcacgcagt tcaacgctga catcaccatt ggccaccacc tgccagtcaa
    12541 cagacgcgtg gttacagtct tgcgcgacat gcgtcaccac ggtgatatcg tccacccagg
    12601 tgttcggcgt ggtgtagagc attacgctgc gatggattcc ggcatagtta aagaaatcat
    12661 ggaagtaaga ctgctttttc ttgccgtttt cgtcggtaat caccattccc ggcgggatag
    12721 tctgccagtt cagttcgttg ttcacacaaa cggtgatacg tacacttttc ccggcaataa
    12781 catacggcgt gacatcggct tcaaatggcg tatagccgcc ctgatgctcc atcacttcct
    12841 gattattgac ccacactttg ccgtaatgag tgaccgcatc gaaacgcagc acgatacgct
    12901 ggcctgccca acctttcggt ataaagactt cgcgctgata ccagacgttg cccgcataat
    12961 tacgaatatc tgcatcggcg aactgatcgt taaaactgcc tggcacagca attgcccggc
    13021 tttcttgtaa cgcgctttcc caccaacgct gatcaattcc acagttttcg cgatccagac
    13081 tgaatgccca caggccgtcg agttttttga tttcacgggt tggggtttct acaggacgga
    13141 cgagtcgtcg gttctgtaac tatcatcatc atcatagaca cacgaaataa agtaatcaga
    13201 ttatcagtta aagctatgta atatttacac cataaccaat caattaaaaa atagatcagt
    13261 ttaaagaaag atcaaagctc aaaaaaataa aaagagaaaa gggtcctaac caagaaaatg
    13321 aaggagaaaa actagaaatt taccctcaga tctaccatgg tcaagagtcc cccgtgttct
    13381 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
    13441 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
    13501 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
    13561 gtcggcagag gcatcttcaa cgatggcctt tcctttatcg caatgatggc atttgtagga
    13621 gccaccttcc ttttccacta tcttcacaat aaagtgacag atagctgggc aatggaatcc
    13681 gaggaggttt ccggatatta ccctttgttg aaaagtctca attgcccttt ggtcttctga
    13741 gactgtatct ttgatatttt tggagtagac aagtgtgtcg tgctccacca tgttgacgaa
    13801 gattttcttc ttgtcattga gtcgtaagag actctgtatg aactgttcgc cagtctttac
    13861 ggcgagttct gttaggtcct ctatttgaat ctttgactcc atgaagctaa actgaaggcg
    13921 ggaaacgaca atctgatcca agctcaagct gctctagcat tcgccattca ggctgcgcaa
    13981 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    14041 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    14101 aacgacggcc agtgccaagc ttgcatgcct gcagttaatt aagtttgcaa gcagcagatt
    14161 acgcgcagaa aaaaaggatc tcaaggagat cctttggatc acaaaaaaaa agcccgctca
    14221 ttaggcgggt taaattcccg ggtactgcag ccgattcatt aatgcaggtt aacctggctt
    14281 atcgaaatta atacgactca ctatagggag accggcagac ctgatcccta ctccaaaaat
    14341 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt
    14401 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagtg
    14461 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa
    14521 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggga
    14581 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac
    14641 gtaagggatg acgcacaatc ccacccctac tccaaaaatg tcaaagatac tgtctcagaa
    14701 gaccaaaggg ctat
//
