LOCUS       sequence-174347-        9517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Monticelli S, Rao A
  TITLE     NFAT1 and NFAT2 are positive regulators of IL-4 gene transcription.
  JOURNAL   Eur J Immunol. 2002 Oct . 32(10):2971-8.
  PUBMED    12355451
REFERENCE   2  (bases 1 to 9517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Immunol. 2002 Oct . 32(10):2971-8."
COMMENT     Sequence Label: CA-NFAT2
FEATURES             Location/Qualifiers
     source          1..9517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             106..622
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    686..1027
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1535..1559
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1592..1681
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(4463..4482)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4464..4482)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4492..4509)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4492..4508)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4614..4638)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4670..5236
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4686..5238
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4830..4847)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5057..5076
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5250..5252,5253..5255,5256..5969)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5295..5316)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5556..5575)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5903..5924
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6016..6531
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6693..6709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6717..6723,6724..6741,6742..6747))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6762..6783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6900..6917)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7071..7659)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7151..7170)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7830..8621,8622..8690))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8453..8472
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8691..8795)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8863..8881
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8919..8941)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9041..9060
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9404..9423
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc
       61 atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
      121 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaatacataa
      181 ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa tatgggccaa
      241 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
      301 agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
      361 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
      421 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg
      481 ccagtcctcc gatagactgc gtcgcccggg tacccgtatt cccaataaag cctcttgctg
      541 tttgcatccg aatcgtggac tcgctgatcc ttgggagggt ctcctcagat tgattgactg
      601 cccacctcgg gggtctttca tttggaggtt ccaccgagat ttggagaccc ctgcccaggg
      661 accaccgacc cccccgccgg gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt
      721 ctttgtgcgt gtttgtgccg gcatctaatg tttgcgcctg cgtctgtact agttagctaa
      781 ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac ccggccgcaa
      841 ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct gaggaaggga
      901 gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg agaacctaaa
      961 acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg
     1021 tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc ggaattagat ctctcgaggt catcacaagt ttgtacaaaa aagcaggctt
     1561 cgaaggagat agagcttcca ccatgatctt ttacccatac gatgttcctg actatgcggg
     1621 ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc cagattacgc
     1681 tgctcagtgc ggccgcgaca tgacggggct ggagcaggac ccggagttcg acttcgattt
     1741 cctcttcgag ttcgatcaga gcggcggggg cgccgcggct gcagaacact acagttatgt
     1801 gtcccctagt gtcacctcga ccctgcccct tcccacagca cactctgcct tgccagcagc
     1861 atgccacgac ctccagacgt ccaccccggg tatctcagct gttccttcag ccaatcatcc
     1921 ccccagttac ggaggggctg tggacagcgg gccttcggga tacttcctgt cctctggcaa
     1981 caccagaccc aacggggccc cgactctgga gagtccgaga atcgagatca cctcctacct
     2041 gggcctacac catggcagcg gccaattttt ccacgacgtg gaggtggaag acgtacttcc
     2101 tagctgcaag cgctcaccgt ctacagcaac cctgcacctg cccagcctgg aagcctacag
     2161 agacccctcc tgcctggccc cagcggccag tctcgcctcc agagcctgta acgctgaggc
     2221 cgcctcctac gagtccaact actcctaccc atacgcggcc ccccagaccg ctccatggca
     2281 ggcaccctgc gtggctccca agaccacgga cccggaggag ggttttcccc gaagcctggg
     2341 tgcctgccac ctgctaggag cgcctaggca cgccccatcc accgctcctc gggcaagcat
     2401 cacggaggag agctggctcg gtgcccgcgg ctcccggccc acgtccccct gcaacaagcg
     2461 caagtacagt ctcaatggcc ggcagccctc ctgcgcaccc caccacgcac ccacaccagc
     2521 cccccatggc gcccctcggg tcagtgtgac cgaagatacc tggctcggta acaccaccca
     2581 gtataccagc tctgccattg tggcagccat caacgccctg accaccgata gcactctgga
     2641 cctgggtgat ggggtcccta tcaagtctcg aaagacagca ctggagcatg cgccctctgt
     2701 ggccctcaaa gtagagccag ctggggaaga cctgggcacc actccaccca cttctgactt
     2761 cccacccgag gagtacacct tccagcacct tcggaagggt gccttttgcg agcagtatct
     2821 gtcggtgcca caggcctcgt atcagtgggc gaagcccaag tctctttccc cgacatcata
     2881 tatgagccca tccttgcctg cccttgactg gcagctcccg tcacattctg gtccatacga
     2941 gcttcggatc gaggtgcagc ccaagtctca ccacagggct cactatgaga cggaaggcag
     3001 ccggggggct gtgaaggctt cagctggagg acaccccatt gtgcagctac acggttactt
     3061 ggagaatgaa cctctcacgc tacagctgtt cattgggacg gctgacgacc gcctgctgag
     3121 gccccacgcc ttctaccagg tccaccggat cacggggaag accgtctcca ccaccagcca
     3181 cgagatcatc ctgtccaaca ccaaagtcct ggagatcccg ttgcttccag aaaataacat
     3241 gcgagccatc atcgactgtg ctgggatcct gaagctcaga aactctgata ttgagctgag
     3301 gaaaggggag acagacatcg ggaggaagaa caccagggtg aggctggtct tccgagttca
     3361 catcccacag cccaatggcc ggacgctgtc tctccaggtg gcctcgaacc ctatcgagtg
     3421 ttcccagcgg tcagcccagg agctgcccct cgtggagaag cagagcacag acagctaccc
     3481 agtcatcggc gggaagaaga tggtgctgtc tggccataac tttctgcaag actccaaagt
     3541 cattttcgtg gagaaggctc cagatggcca ccacgtctgg gagatggaag caaagactga
     3601 ccgggacctg tgcaagccaa attccctggt ggttgagata ccacctttcc gcaaccagag
     3661 gataaccagc cccgtccaag tcagtttcta tgtctgcaac gggaaacgga agagaagcca
     3721 gtaccagcgt ttcacgtacc ttcctgccaa tggtaactct gtctttctaa ccttaagctc
     3781 tgagagtgag ctgagaggag gtttttactg agcagccgag gctataagag gatgttgttg
     3841 taaacaaaac aaaacaaaac aaaacctacc tgtagcctct tcacaccacg cgatagccct
     3901 attcacaaga ccaagtcgcc caccccctca aagaaaagcg aagcctgggt gtgttttcct
     3961 gtgactggtg catgctgggg tcatcacttg ctcgcctttt gcaaatacag cagcgcggcc
     4021 aaccaagcag ctctgctgcg ctcaggggct gatgcggtct gggggtgtat atctaacctc
     4081 tgtgagtctt tgggttagaa gaaagtattt gtcaacgcag ttttgtaagt agcttcgaaa
     4141 ataagcctgc cgtggtcact ggggaacata catgatgttg tcctcatggt gacgcttcta
     4201 cacagcgtgc ggtgtgtctc cactgaataa tgctgtcccc tggtgacgtg agactttcag
     4261 atggaagctc ttctgctcga gtttactcat ttagggaatg gcttctttca ttcagaagtg
     4321 atcggctcgc cttttcaact ttctagggtg ttttatttac gaaaataccg tttttaactg
     4381 ctcccccccc ccctgacgta gctcgaggga tccactagtc tgcagtctag agcggccgcc
     4441 accgcggtgg agctccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     4501 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     4561 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc tagacccagc
     4621 tttcttgtac aaagtggtga tgacggtatc gataagcttg atatcgaatt ccgccccccc
     4681 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     4741 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     4801 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     4861 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     4921 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     4981 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     5041 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     5101 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     5161 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     5221 aaaaacacga tgataatatg gccacaacca tggtgagcaa gggcgaggag ctgttcaccg
     5281 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     5341 ctggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     5401 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     5461 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     5521 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     5581 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     5641 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     5701 tctatatcat ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca
     5761 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     5821 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     5881 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     5941 ctctcggcat ggacgagctg tacaagtaat gagataaaat aaaagatttt atttagtctc
     6001 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     6061 ccattttgca aggcatggaa aatacataac tgagaataga gaagttcaga tcaaggttag
     6121 gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     6181 cggctcaggg ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga
     6241 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     6301 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     6361 aaagagccca caacccctca ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt
     6421 acccgtattc ccaataaagc ctcttgctgt ttgcatccga cttgtggtct cgctgttcct
     6481 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tgggtaacag
     6541 tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag taatcctgac
     6601 tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt tcattatgga
     6661 cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat tccacacaac
     6721 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6781 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6841 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6901 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6961 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7021 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7081 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7141 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7201 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7261 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7321 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7381 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7441 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7501 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7561 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7621 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7681 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7741 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7801 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7861 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7921 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7981 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8041 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8101 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8161 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8221 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8281 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8341 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8401 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8461 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8521 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8581 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8641 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8701 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8761 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8821 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8881 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     8941 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9001 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     9061 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     9121 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     9181 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     9241 acgccagggt tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca
     9301 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
     9361 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
     9421 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
     9481 ggcgattagt ccaatttgtt aaagacagga tatcagt
//
