LOCUS       sequence-174350-       14461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disturb the expression of miR166 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14461)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM166
FEATURES             Location/Qualifiers
     source          1..14461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    525..701
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1263..1281)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1264..1281)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(1352..2011)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKEITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1926..1945
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2048..2053,2054..2070,2071..2076))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2382..2399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2382..2398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2391..2413)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2775..3120
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3137..3151,3342..5159)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          3152..3341
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             5166..5183
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5218..5470
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5492..5516
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6817..7446
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7875..8948
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9014..9208
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9444..9466)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9552..9692
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9607..9626
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9707..9724)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9878..10466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10553..11347)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11772..11796
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11874..12048
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12089..13114)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(13187..13532)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    13723..13744
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13759..13764,13765..13782,13783..13789)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13797..13813
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13802..13824
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13821..13837
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13821..13837
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14036..14055
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14036..14054
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctac
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcatcg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttggta
      361 ccggggaatg aagctacctg gtccgagttg ttgttgttat ggtctaattt aaatatggtc
      421 taaagaagaa gaatggggaa tgaagctacc tggtccgagg atccactagt gaattcggta
      481 cgctgaaatc accagtctct ctctacaaat ctatctctct ctattttctc cataaataat
      541 gtgtgagtag tttcccgata agggaaatta gggttcttat agggtttcgc tcatgtgttg
      601 agcatataag agacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
      661 tctaattcct aaaaccaaaa tccagtacta aaatccagat ctcctaaagt ccctatagat
      721 ctttgtcgtg aatataaacc agacacgaga cgactagacc tggagcccag acgccgttcg
      781 aagctagaag taccgcttag gcaggaggcc gttagggaaa agatgctaag gcagggttgg
      841 ttacgttgac tcccccgtag gtttggttta aatatgatga agtggacgga aggaaggagg
      901 aagacaagga aggataaggt tgcaggccct gtgcaaggta agaagatgga aatttgatag
      961 aggtacgcta ctatacttat actatacgct aagggaatga ttgtatttat accctatacc
     1021 ccctaataac cccttatcaa tttaagaaat aatccgcata agcccccgct taaaaattgg
     1081 tatcagagcc atgaataggt ctatgaccaa aactcaagag gataaaacct caccaaaata
     1141 cgaaagagtc cttaactcta aagataaaag atctttcaag atcaaaacta gttccctcac
     1201 accggtgacg gggatcgcat gcgacctcta gaccatcaca ttggagcttc agctgctcga
     1261 gttctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     1321 ccgcgttcta acgtcgacag gcctgtacca attacgcccc gccctgccac tcatcgcagt
     1381 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     1441 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1501 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1561 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1621 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1681 cgtggtactc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1741 aagggtgaac actatcccat attaccagct caccgtcttt cattgccata cggaactccg
     1801 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1861 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1921 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1981 cggtggtata tccagtgatt tctttctcca ttttagcttc cttagctcct gaaaatctcg
     2041 ataactcatt aaaacttatc gatgataagc tgtcaaacat gagaatttag cccgcctaat
     2101 gagcgggctt tttgctgtga tccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2161 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2221 gataccaggc gtttcccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2281 cttaccggat acctgtccgc ctttcttaat taagtttgca agcagcagat tacggatacc
     2341 tgtccgcctt tcttaattaa ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
     2401 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     2461 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     2521 agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt
     2581 cagtttagct tcatggagtc aaagattcaa atagaggacc taacagaact cgccgtaaag
     2641 actggcgaac agttcataca gagtctctta cgactcaatg acaagaagaa aatcttcgtc
     2701 aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa
     2761 gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc
     2821 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac
     2881 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt
     2941 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
     3001 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     3061 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagagaaca
     3121 cgggggactc ttgaccatgg tagatctgag ggtaaatttc tagtttttct ccttcatttt
     3181 cttggttagg acccttttct ctttttattt ttttgagctt tgatctttct ttaaactgat
     3241 ctatttttta attgattggt tatggtgtaa atattacata gctttaactg ataatctgat
     3301 tactttattt cgtgtgtcta tgatgatgat gatagttaca gaaccgacga ctcgtccgtc
     3361 ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg
     3421 atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg
     3481 caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt cgtaattatg
     3541 cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca ggccagcgta
     3601 tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat aatcaggaag
     3661 tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg tatgttattg
     3721 ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg cagactatcc
     3781 cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt
     3841 tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg aacacctggg
     3901 tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg tctgttgact
     3961 ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg
     4021 ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac ctctggcaac
     4081 cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca gagtgtgata
     4141 tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag ttcctgatta
     4201 accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac ttgcgtggca
     4261 aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg attggggcca
     4321 actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg gcagatgaac
     4381 atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct ttaggcattg
     4441 gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc aacggggaaa
     4501 ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa aaccacccaa
     4561 gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt gcacgggaat
     4621 atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg
     4681 tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt gatgtgctgt
     4741 gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg gcagagaagg
     4801 tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt atcatcaccg
     4861 aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg tggagtgaag
     4921 agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg
     4981 tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg
     5041 gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc
     5101 tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaag
     5161 ctagccacca ccaccaccac cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat
     5221 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     5281 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     5341 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     5401 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     5461 ttactagatc gggaattaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     5521 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     5581 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
     5641 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
     5701 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
     5761 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
     5821 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
     5881 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
     5941 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
     6001 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
     6061 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
     6121 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
     6181 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
     6241 ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
     6301 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
     6361 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
     6421 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
     6481 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
     6541 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
     6601 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
     6661 tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct
     6721 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
     6781 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
     6841 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
     6901 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
     6961 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
     7021 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
     7081 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
     7141 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
     7201 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
     7261 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
     7321 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
     7381 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
     7441 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
     7501 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
     7561 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
     7621 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
     7681 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
     7741 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
     7801 tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag
     7861 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     7921 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
     7981 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     8041 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     8101 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     8161 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     8221 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
     8281 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     8341 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     8401 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     8461 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     8521 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     8581 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     8641 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     8701 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     8761 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     8821 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     8881 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     8941 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     9001 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
     9061 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
     9121 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
     9181 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
     9241 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
     9301 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
     9361 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
     9421 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     9481 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     9541 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     9601 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     9661 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     9721 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9781 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9841 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9901 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9961 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10021 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10081 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10141 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10201 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10261 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    10321 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10381 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10441 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10501 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca
    10561 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt
    10621 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga
    10681 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta
    10741 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt
    10801 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag
    10861 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca
    10921 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt
    10981 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt
    11041 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat
    11101 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca
    11161 tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta
    11221 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg
    11281 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt
    11341 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
    11401 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
    11461 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
    11521 tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
    11581 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
    11641 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
    11701 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
    11761 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
    11821 aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg
    11881 attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca
    11941 aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga
    12001 accctaattc ccttatctgg gaactactca cacattatta tggagaaact cgagcttgtc
    12061 gatcgacaga tcccggtcgg catctactct atttctttgc cctcggacga gtgctggggc
    12121 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc
    12181 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc
    12241 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
    12301 caagaccaat gcggagcata tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc
    12361 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga
    12421 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg
    12481 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc
    12541 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg
    12601 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc
    12661 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc
    12721 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat agcctccgcg accggttgta
    12781 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gaacggcggg
    12841 agatgcaata ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc ggaatcggga
    12901 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc
    12961 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt
    13021 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
    13081 cgacagacgt cgcggtgagt tcaggctttt tcatatctca ttgccccccg gatctgcgaa
    13141 agctcgagag agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat
    13201 gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat
    13261 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
    13321 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
    13381 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
    13441 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
    13501 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
    13561 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta
    13621 gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    13681 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    13741 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    13801 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt cgagctcggt
    13861 acccggggat cctctagcta gattaattaa gtttgcaagc agcagattac gcgcagaaaa
    13921 aaaggatctc aaggagatcc tttggatcac aaaaaaaagc ccgctcatta ggcgggttaa
    13981 attcccgggt actgcagccg attcattaat gcaggttaac ctggcttatc gaaattaata
    14041 cgactcacta tagggagacc ggtagatctg atccctactc caaaaatgtc aaagatacag
    14101 tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatttcg ggaaacctcc
    14161 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtggaa aaggaaggtg
    14221 gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat gcctctgccg
    14281 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtgggaaaa gaagacgttc
    14341 caaccacgtc ttcaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg
    14401 cacaatcccg cccctactcc aaaaatgtca aagatactgt ctcagaagac caaagggcta
    14461 t
//
