LOCUS       sequence-174359-       14484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disturb the expression of miR394 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14484)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM394
FEATURES             Location/Qualifiers
     source          1..14484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    504..680
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1242..1260)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1243..1260)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(join(2021..2026,2027..2043,2044..2049))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2380..2396)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2380..2396)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2393..2415)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2404..2420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2428..2434,2435..2452,2453..2458))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2473..2494
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2685..3030
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3103..4128
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4169..4343
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4421..4445
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4870..5664
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5751..6339
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6493..6510
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6525..6665
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6591..6610)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6751..6773
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7009..7203
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7269..8342)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8771..9400)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10701..10725
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(10747..10999)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11034..11051)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(11058..12875,13066..13080))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          12876..13065
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(13097..13442)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     13804..13826
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13818..13835
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13819..13835
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14059..14078
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14059..14077
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttggag
      361 gtgacctaag aatgccaagt tgttgttgtt atggtctaat ttaaatatgg tctaaagaag
      421 aagaatggag gtggacctaa gaatgccaag aattcggtac gctgaaatca ccagtctctc
      481 tctacaaatc tatctctctc tattttctcc ataaataatg tgtgagtagt ttcccgataa
      541 gggaaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
      601 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
      661 ccagtactaa aatccagatc tcctaaagtc cctatagatc tttgtcgtga atataaacca
      721 gacacgagac gactagacct ggagcccaga cgccgttcga agctagaagt accgcttagg
      781 caggaggccg ttagggaaaa gatgctaagg cagggttggt tacgttgact cccccgtagg
      841 tttggtttaa atatgatgaa gtggacggaa ggaaggagga agacaaggaa ggataaggtt
      901 gcaggccctg tgcaaggtaa gaagatggaa atttgataga ggtacgctac tatacttata
      961 ctatacgcta agggaatgat tgtatttata ccctataccc cctaataacc ccttatcaat
     1021 ttaagaaata atccgcataa gcccccgctt aaaaattggt atcagagcca tgaataggtc
     1081 tatgaccaaa actcaagagg ataaaacctc accaaaatac gaaagagtcc ttaactctaa
     1141 agataaaaga tctttcaaga tcaaaactag ttccctcaca ccggtgacgg ggatcgcatg
     1201 cgacctctag accatcacat tggagcttca gctgctcgag ttctatagtg tcacctaaat
     1261 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctta cgtcgacgta
     1321 ccaattacgc cccgccctgt cactcatcgc agtactgttg taattcatta agcattctgc
     1381 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     1441 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     1501 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     1561 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     1621 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1681 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catattacca
     1741 gctcaccgtc tttcattgcc atacggaact ccggatgagc attcatcagg cgggcaagaa
     1801 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     1861 taatatccag ctgaacggtc tggttatagg tacattgagc aactgaccga aatgcctcaa
     1921 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttctttct
     1981 ccattttagc ttccttagct cctgaaaatc tcgataactc attaaagctt atcgatgata
     2041 agctgtcaaa catgagaatt tagcccgcct aatgagcggg ctttttgttg tgatccgcgt
     2101 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc ccctggaagc
     2221 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctt
     2281 aattaagttt gcaagcagca gattaccgga tacctgtccg cctttcttaa ttaatctagc
     2341 tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg tcatagctgt ttcctgtgtg
     2401 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     2461 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2521 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2581 cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
     2641 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
     2701 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
     2761 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
     2821 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     2881 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     2941 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     3001 taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat
     3061 ctatctctct cgagctttcg cagatccggg gggcaatgag atatgaaaaa gcctgaactc
     3121 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     3181 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     3241 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     3301 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt tagcgagagc
     3361 ctgacctatt gcatctcccg ccgttcacag ggtgtcacgt tgcaagacct gcctgaaacc
     3421 gaactgcccg ctgttctaca accggtcgcg gaggctatgg atgcgatcgc tgcggccgat
     3481 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     3541 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     3601 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     3661 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     3721 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     3781 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     3841 cgctacttcg agcggaggca tccggagctt gcaggatcgc cacgactccg ggcgtatatg
     3901 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     3961 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     4021 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     4081 gatagtggaa accgacgccc cagcactcgt ccgagggcaa agaaatagag tagatgccga
     4141 ccgggatctg tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
     4201 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
     4261 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     4321 aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa
     4381 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     4441 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     4501 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     4561 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     4621 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     4681 tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
     4741 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
     4801 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
     4861 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
     4921 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
     4981 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
     5041 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
     5101 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
     5161 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
     5221 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
     5281 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
     5341 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
     5401 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
     5461 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
     5521 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
     5581 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
     5641 attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag
     5701 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5761 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5821 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5881 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5941 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6001 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6061 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6121 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6181 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6241 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6301 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6361 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6421 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6481 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     6541 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     6601 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     6661 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     6721 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     6781 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     6841 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     6901 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     6961 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     7021 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     7081 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     7141 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     7201 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     7261 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     7321 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     7381 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     7441 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     7501 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     7561 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     7621 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     7681 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     7741 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     7801 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     7861 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     7921 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     7981 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     8041 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     8101 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
     8161 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     8221 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     8281 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     8341 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag gcaacccagc
     8401 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     8461 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     8521 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     8581 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     8641 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     8701 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     8761 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     8821 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     8881 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     8941 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     9001 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
     9061 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
     9121 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
     9181 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
     9241 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
     9301 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
     9361 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
     9421 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
     9481 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
     9541 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
     9601 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
     9661 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
     9721 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
     9781 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
     9841 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
     9901 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
     9961 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    10021 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    10081 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
    10141 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
    10201 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
    10261 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
    10321 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
    10381 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
    10441 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
    10501 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
    10561 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
    10621 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
    10681 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
    10741 attcccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct
    10801 atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc
    10861 atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca
    10921 gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt
    10981 gccaaatgtt tgaacgatcg gggaaattcg agctggtcac ctgtaattca cacgtggtgg
    11041 tggtggtggt ggctagcttg tttgcctccc tgctgcggtt tttcaccgaa gttcatgcca
    11101 gtccagcgtt tttgcagcag aaaagccgcc gacttcggtt tgcggtcgcg agtgaagatc
    11161 cctttcttgt taccgccaac gcgcaatatg ccttgcgagg tcgcaaaatc ggcgaaattc
    11221 catacctgtt caccgacgac ggcgctgacg cgatcaaaga cgcggtgata catatccagc
    11281 catgcacact gatactcttc actccacatg tcggtgtaca ttgagtgcag cccggctaac
    11341 gtatccacgc cgtattcggt gatgataatc ggctgatgca gtttctcctg ccaggccaga
    11401 agttcttttt ccagtacctt ctctgccgtt tccaaatcgc cgctttggac ataccatccg
    11461 taataacggt tcaggcacag cacatcaaag agatcgctga tggtatcggt gtgagcgtcg
    11521 cagaacatta cattgacgca ggtgatcgga cgcgtcgggt cgagtttacg cgttgcttcc
    11581 gccagtggcg cgaaatattc ccgtgcacct tgcggacggg tatccggttc gttggcaata
    11641 ctccacatca ccacgcttgg gtggtttttg tcacgcgcta tcagctcttt aatcgcctgt
    11701 aagtgcgctt gctgagtttc cccgttgact gcctcttcgc tgtacagttc tttcggcttg
    11761 ttgcccgctt cgaaaccaat gcctaaagag aggttaaagc cgacagcagc agtttcatca
    11821 atcaccacga tgccatgttc atctgcccag tcgagcatct cttcagcgta agggtaatgc
    11881 gaggtacggt aggagttggc cccaatccag tccattaatg cgtggtcgtg caccatcagc
    11941 acgttatcga atcctttgcc acgcaagtcc gcatcttcat gacgaccaaa gccagtaaag
    12001 tagaacggtt tgtggttaat caggaactgt tcgcccttca ctgccactga ccggatgccg
    12061 acgcgaagcg ggtagatatc acactctgtc tggcttttgg ctgtgacgca cagttcatag
    12121 agataacctt cacccggttg ccagaggtgc ggattcacca cttgcaaagt cccgctagtg
    12181 ccttgtccag ttgcaaccac ctgttgatcc gcatcacgca gttcaacgct gacatcacca
    12241 ttggccacca cctgccagtc aacagacgcg tggttacagt cttgcgcgac atgcgtcacc
    12301 acggtgatat cgtccaccca ggtgttcggc gtggtgtaga gcattacgct gcgatggatt
    12361 ccggcatagt taaagaaatc atggaagtaa gactgctttt tcttgccgtt ttcgtcggta
    12421 atcaccattc ccggcgggat agtctgccag ttcagttcgt tgttcacaca aacggtgata
    12481 cgtacacttt tcccggcaat aacatacggc gtgacatcgg cttcaaatgg cgtatagccg
    12541 ccctgatgct ccatcacttc ctgattattg acccacactt tgccgtaatg agtgaccgca
    12601 tcgaaacgca gcacgatacg ctggcctgcc caacctttcg gtataaagac ttcgcgctga
    12661 taccagacgt tgcccgcata attacgaata tctgcatcgg cgaactgatc gttaaaactg
    12721 cctggcacag caattgcccg gctttcttgt aacgcgcttt cccaccaacg ctgatcaatt
    12781 ccacagtttt cgcgatccag actgaatgcc cacaggccgt cgagtttttt gatttcacgg
    12841 gttggggttt ctacaggacg gacgagtcgt cggttctgta actatcatca tcatcataga
    12901 cacacgaaat aaagtaatca gattatcagt taaagctatg taatatttac accataacca
    12961 atcaattaaa aaatagatca gtttaaagaa agatcaaagc tcaaaaaaat aaaaagagaa
    13021 aagggtccta accaagaaaa tgaaggagaa aaactagaaa tttaccctca gatctaccat
    13081 ggtcaagagt cccccgtgtt ctctccaaat gaaatgaact tccttatata gaggaagggt
    13141 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
    13201 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
    13261 ggggtccatc tttgggacca ctgtcggcag aggcatcttc aacgatggcc tttcctttat
    13321 cgcaatgatg gcatttgtag gagccacctt ccttttccac tatcttcaca ataaagtgac
    13381 agatagctgg gcaatggaat ccgaggaggt ttccggatat taccctttgt tgaaaagtct
    13441 caattgccct ttggtcttct gagactgtat ctttgatatt tttggagtag acaagtgtgt
    13501 cgtgctccac catgttgacg aagattttct tcttgtcatt gagtcgtaag agactctgta
    13561 tgaactgttc gccagtcttt acggcgagtt ctgttaggtc ctctatttga atctttgact
    13621 ccatgaagct aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc
    13681 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    13741 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    13801 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagttaa
    13861 ttaagaaagg cggacaggta tcccgtaatc tgctgcttgc aaacttaatt aagtttgcaa
    13921 gcagcagatt acgcgcagaa aaaaaggatc tcaaggagat cctttggatc acaaaaaaaa
    13981 agcccgctca ttaggcgggt taaattcccg ggtactgcag ccgattcatt aatgcaggtt
    14041 aacctggctt atcgaaatta atacgactca ctatagggag accggcagat ctgatcccta
    14101 ctccaaaaat gtcaaagata cggtctcaga agaccaaagg gctattgaga cttttcaaca
    14161 aagggtaatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga
    14221 aaggacagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
    14281 tatcattcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
    14341 catcgtggga aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat
    14401 ctccactgac gtaagggatg acgcacaatc ccacccctac tccaaaaatg tcaaagatac
    14461 tgtctcagaa gaccaaaggg ctat
//
