LOCUS       sequence-174363-       14716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disturb the expression of miR528 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14716)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM528
FEATURES             Location/Qualifiers
     source          1..14716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     RBS             317..325
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2335..2351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2335..2351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2348..2370)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2359..2375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2383..2389,2390..2407,2408..2413))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2428..2449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2640..3316
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3383..4408
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4449..4623
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4701..4725
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5150..5944
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6031..6619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6773..6790
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6805..6945
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6871..6890)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7031..7053
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7289..7483
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7549..8622)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9051..9680)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10981..11005
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11027..11279)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11314..11331)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(11338..13155,13346..13360))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          13156..13345
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(13377..13722)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     14084..14106
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14098..14115
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14099..14115
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14291..14310
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14291..14309
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaggcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaggttcatt tcatttggag aggacagccc aagcttctcc
      361 tctgcatcta gccccttcca gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatct cctctgcatc tagccccttc cagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cccggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacccc tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc agacgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaat ctagctagag gatccccggg taccgagctc gaattcgtaa tcatgtcata
     2341 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2401 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2461 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2521 acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc ttgccaacat ggtggagcac
     2581 gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt
     2641 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
     2701 tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc
     2761 gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc
     2821 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     2881 gattgatgtg aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
     2941 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
     3001 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
     3061 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc
     3121 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     3181 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
     3241 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     3301 ggagaggaca cgctgaaatc accagtctct ctctacaaat ctatctctct cgagctttcg
     3361 cagatccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     3421 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     3481 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     3541 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     3601 gattccggaa gtgcttgaca ttggggagtt tagcgagagc ctgacctatt gcatctcccg
     3661 ccgttcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctaca
     3721 accggtcgcg gaggctatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     3781 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     3841 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     3901 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     3961 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     4021 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     4081 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     4141 tccggagctt gcaggatcgc cacgactccg ggcgtatatg ctccgcattg gtcttgacca
     4201 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     4261 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     4321 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     4381 cagcactcgt ccgagggcaa agaaatagag tagatgccga ccgggatctg tcgatcgaca
     4441 agctcgagtt tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct
     4501 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
     4561 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
     4621 atcccccgaa ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
     4681 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     4741 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     4801 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     4861 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     4921 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg
     4981 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
     5041 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
     5101 cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat
     5161 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
     5221 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
     5281 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
     5341 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
     5401 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
     5461 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
     5521 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
     5581 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc
     5641 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
     5701 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
     5761 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
     5821 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
     5881 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
     5941 ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6001 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6061 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6121 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6181 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6241 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6301 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6361 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6421 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6481 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6541 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6601 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6661 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6721 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6781 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     6841 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6901 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     6961 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     7021 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     7081 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     7141 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     7201 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     7261 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     7321 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     7381 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     7441 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     7501 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     7561 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     7621 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     7681 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     7741 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     7801 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     7861 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     7921 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     7981 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     8041 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     8101 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
     8161 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
     8221 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
     8281 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
     8341 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
     8401 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
     8461 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
     8521 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
     8581 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
     8641 ggggttccag tgccattgca gggccggcag gcaacccagc cgcttacgcc tggccaaccg
     8701 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
     8761 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
     8821 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
     8881 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
     8941 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
     9001 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
     9061 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
     9121 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
     9181 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
     9241 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
     9301 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
     9361 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
     9421 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
     9481 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
     9541 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
     9601 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
     9661 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
     9721 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
     9781 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
     9841 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
     9901 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
     9961 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    10021 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    10081 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    10141 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    10201 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    10261 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    10321 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    10381 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    10441 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    10501 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    10561 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    10621 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    10681 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    10741 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    10801 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    10861 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
    10921 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
    10981 gtttacccgc caatatatcc tgtcaaacac tgatagttta attcccgatc tagtaacata
    11041 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
    11101 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
    11161 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
    11221 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatcg
    11281 gggaaattcg agctggtcac ctgtaattca cacgtggtgg tggtggtggt ggctagcttg
    11341 tttgcctccc tgctgcggtt tttcaccgaa gttcatgcca gtccagcgtt tttgcagcag
    11401 aaaagccgcc gacttcggtt tgcggtcgcg agtgaagatc cctttcttgt taccgccaac
    11461 gcgcaatatg ccttgcgagg tcgcaaaatc ggcgaaattc catacctgtt caccgacgac
    11521 ggcgctgacg cgatcaaaga cgcggtgata catatccagc catgcacact gatactcttc
    11581 actccacatg tcggtgtaca ttgagtgcag cccggctaac gtatccacgc cgtattcggt
    11641 gatgataatc ggctgatgca gtttctcctg ccaggccaga agttcttttt ccagtacctt
    11701 ctctgccgtt tccaaatcgc cgctttggac ataccatccg taataacggt tcaggcacag
    11761 cacatcaaag agatcgctga tggtatcggt gtgagcgtcg cagaacatta cattgacgca
    11821 ggtgatcgga cgcgtcgggt cgagtttacg cgttgcttcc gccagtggcg cgaaatattc
    11881 ccgtgcacct tgcggacggg tatccggttc gttggcaata ctccacatca ccacgcttgg
    11941 gtggtttttg tcacgcgcta tcagctcttt aatcgcctgt aagtgcgctt gctgagtttc
    12001 cccgttgact gcctcttcgc tgtacagttc tttcggcttg ttgcccgctt cgaaaccaat
    12061 gcctaaagag aggttaaagc cgacagcagc agtttcatca atcaccacga tgccatgttc
    12121 atctgcccag tcgagcatct cttcagcgta agggtaatgc gaggtacggt aggagttggc
    12181 cccaatccag tccattaatg cgtggtcgtg caccatcagc acgttatcga atcctttgcc
    12241 acgcaagtcc gcatcttcat gacgaccaaa gccagtaaag tagaacggtt tgtggttaat
    12301 caggaactgt tcgcccttca ctgccactga ccggatgccg acgcgaagcg ggtagatatc
    12361 acactctgtc tggcttttgg ctgtgacgca cagttcatag agataacctt cacccggttg
    12421 ccagaggtgc ggattcacca cttgcaaagt cccgctagtg ccttgtccag ttgcaaccac
    12481 ctgttgatcc gcatcacgca gttcaacgct gacatcacca ttggccacca cctgccagtc
    12541 aacagacgcg tggttacagt cttgcgcgac atgcgtcacc acggtgatat cgtccaccca
    12601 ggtgttcggc gtggtgtaga gcattacgct gcgatggatt ccggcatagt taaagaaatc
    12661 atggaagtaa gactgctttt tcttgccgtt ttcgtcggta atcaccattc ccggcgggat
    12721 agtctgccag ttcagttcgt tgttcacaca aacggtgata cgtacacttt tcccggcaat
    12781 aacatacggc gtgacatcgg cttcaaatgg cgtatagccg ccctgatgct ccatcacttc
    12841 ctgattattg acccacactt tgccgtaatg agtgaccgca tcgaaacgca gcacgatacg
    12901 ctggcctgcc caacctttcg gtataaagac ttcgcgctga taccagacgt tgcccgcata
    12961 attacgaata tctgcatcgg cgaactgatc gttaaaactg cctggcacag caattgcccg
    13021 gctttcttgt aacgcgcttt cccaccaacg ctgatcaatt ccacagtttt cgcgatccag
    13081 actgaatgcc cacaggccgt cgagtttttt gatttcacgg gttggggttt ctacaggacg
    13141 gacgagtcgt cggttctgta actatcatca tcatcataga cacacgaaat aaagtaatca
    13201 gattatcagt taaagctatg taatatttac accataacca atcaattaaa aaatagatca
    13261 gtttaaagaa agatcaaagc tcaaaaaaat aaaaagagaa aagggtccta accaagaaaa
    13321 tgaaggagaa aaactagaaa tttaccctca gatctaccat ggtcaagagt cccccgtgtt
    13381 ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat
    13441 tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg
    13501 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
    13561 ctgtcggcag aggcatcttc aacgatggcc tttcctttat cgcaatgatg gcatttgtag
    13621 gagccacctt ccttttccac tatcttcaca ataaagtgac agatagctgg gcaatggaat
    13681 ccgaggaggt ttccggatat taccctttgt tgaaaagtct caattgccct ttggtcttct
    13741 gagactgtat ctttgatatt tttggagtag acaagtgtgt cgtgctccac catgttgacg
    13801 aagattttct tcttgtcatt gagtcgtaag agactctgta tgaactgttc gccagtcttt
    13861 acggcgagtt ctgttaggtc ctctatttga atctttgact ccatgaagct aaactgaagg
    13921 cgggaaacga caatctgatc caagctcaag ctgctctagc attcgccatt caggctgcgc
    13981 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
    14041 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    14101 aaaacgacgg ccagtgccaa gcttgcatgc ctgcagttaa ttaagtttgc aagcagcaga
    14161 ttacgcgcag aaaaaaagga tctcaaggag atcctttgga tcacaaaaaa aaagcccgct
    14221 cattaggcgg gttaaattcc cgggtactgc agccgattca ttaatgcagg ttaacctggc
    14281 ttatcgaaat taatacgact cactataggg agaccggcag atctgatccc tactccaaaa
    14341 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
    14401 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
    14461 tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
    14521 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
    14581 gaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg
    14641 acgtaaggga tgacgcacaa tcccacccct actccaaaaa tgtcaaagat actgtctcag
    14701 aagaccaaag ggctat
//
