LOCUS       sequence-174369-        5406 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  induce expression of dipeptide repeat GR in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Jovicic A, Mertens J, Boeynaems S, Bogaert E, Chai N, Yamada SB, 
            Paul JW 3rd, Sun S, Herdy JR, Bieri G, Kramer NJ, Gage FH, Van Den 
            Bosch L, Robberecht W, Gitler AD
  TITLE     Modifiers of C9orf72 dipeptide repeat toxicity connect 
            nucleocytoplasmic transport defects to FTD/ALS.
  JOURNAL   Nat Neurosci. 2015 Sep;18(9):1226-9. doi: 10.1038/nn.4085.
  PUBMED    26308983
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn"; 
            journalName: "Nat Neurosci"; date: "2015-09"; volume: "18"; issue: 
            "9"; pages: "1226-9"
COMMENT     Sequence Label: pAG303-Gal-GR100
FEATURES             Location/Qualifiers
     source          1..5406
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(97..121)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(154..170)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(155..171)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      173..420
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(189..207)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(456..474)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(495..511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(495..511)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(508..530)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    519..535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(543..549,550..567,568..573))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    588..609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(726..743)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(897..1485)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(977..996)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1656..2447,2448..2516))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2279..2298
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2517..2621)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2689..2707
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2745..2767)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2867..2886
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3033..3220
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             3221..3883
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(4143..4598)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4280..4301)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4492..4511
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4724..4746
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4738..4755
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4739..4755
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4762..4781
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4762..4780
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4800..5241
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    4800..4917
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     5212..5235
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     5256..5272
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5256..5272
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    5280..5304
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaggtagagg tagaggtaga ggtagaggta gaggtagagg tagaggtaga ggtagaggta
       61 gaggtagagg tagaggtaga ggtagaggta gataagaccc agctttcttg tacaaagtgg
      121 tgatgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agtcatgtaa
      181 ttagttatgt cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa
      241 ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta
      301 agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt
      361 aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc
      421 ggcctcgagg gggggcccgg taccagcttt tgttcccttt agtgagggtt aatttcgagc
      481 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      541 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      601 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      661 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      721 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      781 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      841 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      901 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      961 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1021 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1081 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1141 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1201 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1261 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1321 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1381 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1441 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1501 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1561 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1621 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1681 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1741 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1801 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1861 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1921 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1981 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     2041 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     2101 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     2161 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2221 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2281 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2341 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2401 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2461 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2521 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2581 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2641 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2701 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2761 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2821 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2881 attgtactga gagtgcacca taaattcccg ttttaagagc ttggtgagcg ctaggagtca
     2941 ctgccaggta tcgtttgaac acggcattag tcagggaagt cataacacag tcctttcccg
     3001 caattttctt tttctattac tcttggcctc ctctagtaca ctctatattt ttttatgcct
     3061 cggtaatgat tttcattttt ttttttccac ctagcggatg actctttttt tttcttagcg
     3121 attggcatta tcacataatg aattatacat tatataaagt aatgtgattt cttcgaagaa
     3181 tatactaaaa aatgagcagg caagataaac gaaggcaaag atgacagagc agaaagccct
     3241 agtaaagcgt attacaaatg aaaccaagat tcagattgcg atctctttaa agggtggtcc
     3301 cctagcgata gagcactcga tcttcccaga aaaagaggca gaagcagtag cagaacaggc
     3361 cacacaatcg caagtgatta acgtccacac aggtataggg tttctggacc atatgataca
     3421 tgctctggcc aagcattccg gctggtcgct aatcgttgag tgcattggtg acttacacat
     3481 agacgaccat cacaccactg aagactgcgg gattgctctc ggtcaagctt ttaaagaggc
     3541 cctaggggcc gtgcgtggag taaaaaggtt tggatcagga tttgcgcctt tggatgaggc
     3601 actttccaga gcggtggtag atctttcgaa caggccgtac gcagttgtcg aacttggttt
     3661 gcaaagggag aaagtaggag atctctcttg cgagatgatc ccgcattttc ttgaaagctt
     3721 tgcagaggct agcagaatta ccctccacgt tgattgtctg cgaggcaaga atgatcatca
     3781 ccgtagtgag agtgcgttca aggctcttgc ggttgccata agagaagcca cctcgcccaa
     3841 tggtaccaac gatgttccct ccaccaaagg tgttcttatg tagtgacacc gattatttaa
     3901 agctgcagca tacgatatat atacatgtgt atatatgtat acctatgaat gtcagtaagt
     3961 atgtatacga acagtatgat actgaagatg acaaggtaat gcatcattct atacgtgtca
     4021 ttctgaacga ggcgcgcttt ccttttttct ttttgctttt tctttttttt tctcttgaac
     4081 tcgacggatc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     4141 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     4201 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     4261 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     4321 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     4381 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     4441 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     4501 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     4561 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc
     4621 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     4681 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     4741 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattgga gctctagtac
     4801 ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc
     4861 tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac
     4921 aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct
     4981 ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt
     5041 agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta
     5101 acagatatat aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg
     5161 tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct
     5221 ctatacttta acgtcaagga gaaaaaaccc cggattctag aactagtgga tcccccatca
     5281 caagtttgta caaaaaagca ggcttcaaaa atgggtagag gtagaggtag aggtagaggt
     5341 agaggtagag gtagaggtag aggtagaggt agaggtagag gtagaggtag aggtagaggt
     5401 agaggt
//
