LOCUS       sequence-174370-       14763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disturb the expression of miR319 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14763)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM319
FEATURES             Location/Qualifiers
     source          1..14763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    506..682
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1244..1262)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1245..1262)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1901..1920
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2023..2028,2029..2045,2046..2051))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2332..2348)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2332..2348)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2345..2367)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2356..2372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2380..2386,2387..2404,2405..2410))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2425..2446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3301..3977
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4044..5069
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5110..5284
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5362..5386
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5811..6605
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6692..7280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7434..7451
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7466..7606
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7532..7551)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7692..7714
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7950..8144
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8210..9283)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9712..10341)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11642..11666
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11688..11940)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11975..11992)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(11999..13816,14007..14021))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          13817..14006
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(14040..14086)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     14338..14357
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14338..14356
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttttag
      361 ctcccgccta gtcatccagt gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatgg gagcacccct attcagtcca agaattcggt acgctgaaat caccagtctc
      481 tctctacaaa tctatctctc tctattttct ccataaataa tgtgtgagta gtttcccgat
      541 aagggaaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
      601 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
      661 atccagtact aaaatccaga tctcctaaag tccctataga tctttgtcgt gaatataaac
      721 cagacacgag acgactagac ctggagccca gacgccgttc gaagctagaa gtaccgctta
      781 ggcaggaggc cgttagggaa aagatgctaa ggcagggttg gttacgttga ctcccccgta
      841 ggtttggttt aaatatgatg aagtggacgg aaggaaggag gaagacaagg aaggataagg
      901 ttgcaggccc tgtgcaaggt aagaagatgg aaatttgata gaggtacgct actatactta
      961 tactatacgc taagggaatg attgtattta taccctatac cccctaataa ccccttatca
     1021 atttaagaaa taatccgcat aagcccccgc ttaaaaattg gtatcagagc catgaatagg
     1081 tctatgacca aaactcaaga ggataaaacc tcaccaaaat acgaaagagt ccttaactct
     1141 aaagataaaa gatctttcaa gatcaaaact agttccctca caccggtgac ggggatcgca
     1201 tgcgacctct agaccatcac attggagctt cagctgctcg agttctatag tgtcacctaa
     1261 atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct tacgtcgacg
     1321 taccaattac gccccgccct gtcactcatc gcagtactgt tgtaattcat taagcattct
     1381 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1441 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1501 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1561 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1621 ttgcgaatat atgtgtagaa actgccggaa atcgccgtgg tattcactcc agagcgatga
     1681 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatattac
     1741 cagctcaccg tctttcattg ccatgcggaa ctccggatga gcattcatca ggcgggcaag
     1801 aatgtgaata aaggccggat aaaacttgtg cttattcttc tttacggtct ttaaaaaggc
     1861 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1921 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgatttcttt
     1981 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcattaaagc ttatcgatga
     2041 taagctgtca aacatgagaa tttagcccgc ctaatgagcg ggctttttgt tgtgatccgc
     2101 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2161 aagtcagagg tggcgaaacc cgacaggact ataaagatac cgggcgtttc ccccctggaa
     2221 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2281 ttataatcta gctagaggat ccccgggtac cgagctcgaa ttcgtaatca tgtcatagct
     2341 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2401 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2461 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2521 cgcggggaga ggcggtttgc gtattggcta gagcagcttg ccaacatggt ggagcacgac
     2581 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
     2641 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
     2701 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
     2761 aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca
     2821 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     2881 tgatgtgaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
     2941 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
     3001 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
     3061 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
     3121 cagtggtccc aaagatggac ccccacccac gaggagcatc gtgggaaaag aagacgttcc
     3181 aaccacgtct tcaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc
     3241 acaatcccac ccctactcca aaaatgtcaa agatactgtc tcagaagacc aaagggctat
     3301 tgagactttt caacaaaggg taatatcsgg aaacctcctc ggattccatt gcccagctat
     3361 ctgtcacttc atcraaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
     3421 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
     3481 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     3541 ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
     3601 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
     3661 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
     3721 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg
     3781 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     3841 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
     3901 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     3961 tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc tcgagctttc
     4021 gcagatccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     4081 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     4141 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     4201 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     4261 cgattccgga agtgcttgac attggggagt ttagcgagag cctgacctat tgcatctccc
     4321 gccgttcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctac
     4381 aaccggtcgc ggaggctatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     4441 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     4501 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     4561 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     4621 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     4681 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     4741 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     4801 atccggagct tgcaggatcg ccacgactcc gggcgtatat gctccgcatt ggtcttgacc
     4861 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     4921 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     4981 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     5041 ccagcactcg tccgagggca aagaaataga gtagatgccg accgggatct gtcgatcgac
     5101 aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc
     5161 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     5221 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca
     5281 gatcccccga attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt
     5341 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     5401 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     5461 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     5521 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     5581 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc
     5641 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     5701 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     5761 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     5821 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     5881 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     5941 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     6001 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     6061 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     6121 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     6181 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     6241 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     6301 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     6361 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     6421 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     6481 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     6541 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     6601 tttagtacct agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6661 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6721 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6781 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6841 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6901 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6961 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7021 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7081 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7141 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7201 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7261 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7321 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7381 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7441 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     7501 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     7561 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     7621 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     7681 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     7741 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
     7801 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
     7861 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
     7921 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
     7981 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
     8041 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
     8101 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc
     8161 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
     8221 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
     8281 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
     8341 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
     8401 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
     8461 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
     8521 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
     8581 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
     8641 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
     8701 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
     8761 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
     8821 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
     8881 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
     8941 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
     9001 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
     9061 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
     9121 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
     9181 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
     9241 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt
     9301 gggggttcca gtgccattgc agggccggca ggcaacccag ccgcttacgc ctggccaacc
     9361 gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc
     9421 catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact
     9481 ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg
     9541 cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg
     9601 ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc
     9661 cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag
     9721 ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc
     9781 tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat
     9841 acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat
     9901 gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac
     9961 ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct
    10021 tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc
    10081 cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg
    10141 gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc
    10201 ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag
    10261 ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt
    10321 aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc
    10381 atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga
    10441 cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca
    10501 gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac
    10561 acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc
    10621 tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca
    10681 gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg
    10741 gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca
    10801 gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga
    10861 tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct
    10921 cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga
    10981 acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc
    11041 tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc
    11101 tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa
    11161 gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct
    11221 tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc
    11281 tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc
    11341 ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg
    11401 aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag
    11461 aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca
    11521 aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg
    11581 ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta
    11641 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aattcccgat ctagtaacat
    11701 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
    11761 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
    11821 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
    11881 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
    11941 ggggaaattc gagctggtca cctgtaattc acacgtggtg gtggtggtgg tggctagctt
    12001 gtttgcctcc ctgctgcggt ttttcaccga agttcatgcc agtccagcgt ttttgcagca
    12061 gaaaagccgc cgacttcggt ttgcggtcgc gagtgaagat ccctttcttg ttaccgccaa
    12121 cgcgcaatat gccttgcgag gtcgcaaaat cggcgaaatt ccatacctgt tcaccgacga
    12181 cggcgctgac gcgatcaaag acgcggtgat acatatccag ccatgcacac tgatactctt
    12241 cactccacat gtcggtgtac attgagtgca gcccggctaa cgtatccacg ccgtattcgg
    12301 tgatgataat cggctgatgc agtttctcct gccaggccag aagttctttt tccagtacct
    12361 tctctgccgt ttccaaatcg ccgctttgga cataccatcc gtaataacgg ttcaggcaca
    12421 gcacatcaaa gagatcgctg atggtatcgg tgtgagcgtc gcagaacatt acattgacgc
    12481 aggtgatcgg acgcgtcggg tcgagtttac gcgttgcttc cgccagtggc gcgaaatatt
    12541 cccgtgcacc ttgcggacgg gtatccggtt cgttggcaat actccacatc accacgcttg
    12601 ggtggttttt gtcacgcgct atcagctctt taatcgcctg taagtgcgct tgctgagttt
    12661 ccccgttgac tgcctcttcg ctgtacagtt ctttcggctt gttgcccgct tcgaaaccaa
    12721 tgcctaaaga gaggttaaag ccgacagcag cagtttcatc aatcaccacg atgccatgtt
    12781 catctgccca gtcgagcatc tcttcagcgt aagggtaatg cgaggtacgg taggagttgg
    12841 ccccaatcca gtccattaat gcgtggtcgt gcaccatcag cacgttatcg aatcctttgc
    12901 cacgcaagtc cgcatcttca tgacgaccaa agccagtaaa gtagaacggt ttgtggttaa
    12961 tcaggaactg ttcgcccttc actgccactg accggatgcc gacgcgaagc gggtagatat
    13021 cacactctgt ctggcttttg gctgtgacgc acagttcata gagataacct tcacccggtt
    13081 gccagaggtg cggattcacc acttgcaaag tcccgctagt gccttgtcca gttgcaacca
    13141 cctgttgatc cgcatcacgc agttcaacgc tgacatcacc attggccacc acctgccagt
    13201 caacagacgc gtggttacag tcttgcgcga catgcgtcac cacggtgata tcgtccaccc
    13261 aggtgttcgg cgtggtgtag agcattacgc tgcgatggat tccggcatag ttaaagaaat
    13321 catggaagta agactgcttt ttcttgccgt tttcgtcggt aatcaccatt cccggcggga
    13381 tagtctgcca gttcagttcg ttgttcacac aaacggtgat acgtacactt ttcccggcaa
    13441 taacatacgg cgtgacatcg gcttcaaatg gcgtatagcc gccctgatgc tccatcactt
    13501 cctgattatt gacccacact ttgccgtaat gagtgaccgc atcgaaacgc agcacgatac
    13561 gctggcctgc ccaacctttc ggtataaaga cttcgcgctg ataccagacg ttgcccgcat
    13621 aattacgaat atctgcatcg gcgaactgat cgttaaaact gcctggcaca gcaattgccc
    13681 ggctttcttg taacgcgctt tcccaccaac gctgatcaat tccacagttt tcgcgatcca
    13741 gactgaatgc ccacaggccg tcgagttttt tgatttcacg ggttggggtt tctacaggac
    13801 ggacgagtcg tcggttctgt aactatcatc atcatcatag acacacgaaa taaagtaatc
    13861 agattatcag ttaaagctat gtaatattta caccataacc aatcaattaa aaaatagatc
    13921 agtttaaaga aagatcaaag ctcaaaaaaa taaaaagaga aaagggtcct aaccaagaaa
    13981 atgaaggaga aaaactagaa atttaccctc agatctacca tggtcaagag tcccccgtgt
    14041 tctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaga gacaaaaggg
    14101 agacaaaagg gagacaaaag ggagacaaaa gggagacaaa agggagacaa aagggagaca
    14161 aaaggaagct tgcatgcctg cagttaatta agtttgcaag cagcagatta cgcgcagaaa
    14221 aaaaggatct caaggagatc cttcggatca caaaaaaaaa gcccgctcat taggcgggtt
    14281 aaattcccgg gtactgcagc cgattcatta atgcaggtta acctggctta tcgaaattaa
    14341 tacgactcac tatagggaga ccggcagatc tgatccctac tccaaaaatg tcaaagatac
    14401 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaattt cgggaaacct
    14461 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtgg aaaaggaagg
    14521 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atgccactgc
    14581 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtgggaa aagaagacgt
    14641 tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgacg taagggatga
    14701 cgcacaatcc cacccctact ccaaaaatgt caaagatact gtctcagaag accaaagggc
    14761 tat
//
