LOCUS       sequence-174371-       14391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disturb the expression of miR171 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14391)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM171
FEATURES             Location/Qualifiers
     source          1..14391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2335..2351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2335..2351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2348..2370)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2359..2375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2383..2389,2390..2407,2408..2413))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2428..2449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2640..2985
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3058..4083
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4124..4298
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4376..4400
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4825..5619
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5706..6294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6448..6465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6480..6620
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6546..6565)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6706..6728
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6964..7158
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7224..8297)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8726..9355)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10656..10680
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(10702..10954)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(10989..11006)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(11013..12830,13021..13035))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          12831..13020
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(13052..13397)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     13759..13781
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13773..13790
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13774..13790
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13966..13985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13966..13984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 cygtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttgata
      361 ttggcgccta ggctcaatca gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatga tattggcacc taggctcaat cagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactgga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat ggttgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaat ctagctagag gatccccggg taccgagctc gaattcgtaa tcatgtcata
     2341 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2401 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2461 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2521 acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc ttgccaacat ggtggagcac
     2581 gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt
     2641 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
     2701 tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc
     2761 gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc
     2821 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     2881 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     2941 gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg aaatcaccag
     3001 tctctctcta caaatctatc tctctcgagc tttcgcagat ccggggggca atgagatatg
     3061 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     3121 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     3181 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     3241 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     3301 gagtttagcg agagcctgac ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa
     3361 gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg
     3421 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     3481 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     3541 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     3601 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     3661 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     3721 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     3781 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccacga
     3841 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     3901 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     3961 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     4021 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa
     4081 tagagtagat gccgaccggg atctgtcgat cgacaagctc gagtttctcc ataataatgt
     4141 gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc
     4201 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
     4261 aattcctaaa accaaaatcc agtactaaaa tccagatccc ccgaattaat tcggcgttaa
     4321 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
     4381 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
     4441 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
     4501 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
     4561 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
     4621 cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta tgttatacgc
     4681 caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag
     4741 gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg
     4801 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
     4861 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
     4921 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
     4981 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
     5041 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
     5101 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
     5161 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
     5221 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
     5281 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
     5341 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
     5401 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
     5461 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
     5521 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
     5581 aaaataaaat attatatttt actggatgaa ttgttttagt acctagaatg catgaccaaa
     5641 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5701 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5761 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5821 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     5881 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5941 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6001 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6061 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6121 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6181 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6241 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6301 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6361 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6421 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6481 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     6541 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     6601 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6661 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6721 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc
     6781 cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg
     6841 ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg
     6901 gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac
     6961 agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg
     7021 gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg
     7081 tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca
     7141 atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt
     7201 tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg
     7261 cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc
     7321 ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg
     7381 gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg
     7441 gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg
     7501 gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca
     7561 gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg
     7621 ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta
     7681 cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc
     7741 tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac
     7801 acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg
     7861 gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg
     7921 gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca
     7981 cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg
     8041 tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc
     8101 gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct
     8161 tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg
     8221 gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat
     8281 ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc
     8341 ggcaggcaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt
     8401 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa
     8461 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat
     8521 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc
     8581 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac
     8641 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg
     8701 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg
     8761 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg
     8821 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt
     8881 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca
     8941 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca
     9001 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt
     9061 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta
     9121 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga
     9181 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat
     9241 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg
     9301 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt
     9361 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa
     9421 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc
     9481 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag
     9541 ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc
     9601 ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct
     9661 tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc
     9721 tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt
     9781 gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg
     9841 ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg
     9901 gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg
     9961 attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc
    10021 ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg
    10081 atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc
    10141 actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg
    10201 cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg
    10261 gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca
    10321 gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt
    10381 atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc
    10441 gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag
    10501 ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc
    10561 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
    10621 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
    10681 aacactgata gtttaattcc cgatctagta acatagatga caccgcgcgc gataatttat
    10741 cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc
    10801 taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct
    10861 taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc
    10921 ttaagaaact ttattgccaa atgtttgaac gatcggggaa attcgagctg gtcacctgta
    10981 attcacacgt ggtggtggtg gtggtggcta gcttgtttgc ctccctgctg cggtttttca
    11041 ccgaagttca tgccagtcca gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg
    11101 tcgcgagtga agatcccttt cttgttaccg ccaacgcgca atatgccttg cgaggtcgca
    11161 aaatcggcga aattccatac ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg
    11221 tgatacatat ccagccatgc acactgatac tcttcactcc acatgtcggt gtacattgag
    11281 tgcagcccgg ctaacgtatc cacgccgtat tcggtgatga taatcggctg atgcagtttc
    11341 tcctgccagg ccagaagttc tttttccagt accttctctg ccgtttccaa atcgccgctt
    11401 tggacatacc atccgtaata acggttcagg cacagcacat caaagagatc gctgatggta
    11461 tcggtgtgag cgtcgcagaa cattacattg acgcaggtga tcggacgcgt cgggtcgagt
    11521 ttacgcgttg cttccgccag tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc
    11581 ggttcgttgg caatactcca catcaccacg cttgggtggt ttttgtcacg cgctatcagc
    11641 tctttaatcg cctgtaagtg cgcttgctga gtttccccgt tgactgcctc ttcgctgtac
    11701 agttctttcg gcttgttgcc cgcttcgaaa ccaatgccta aagagaggtt aaagccgaca
    11761 gcagcagttt catcaatcac cacgatgcca tgttcatctg cccagtcgag catctcttca
    11821 gcgtaagggt aatgcgaggt acggtaggag ttggccccaa tccagtccat taatgcgtgg
    11881 tcgtgcacca tcagcacgtt atcgaatcct ttgccacgca agtccgcatc ttcatgacga
    11941 ccaaagccag taaagtagaa cggtttgtgg ttaatcagga actgttcgcc cttcactgcc
    12001 actgaccgga tgccgacgcg aagcgggtag atatcacact ctgtctggct tttggctgtg
    12061 acgcacagtt catagagata accttcaccc ggttgccaga ggtgcggatt caccacttgc
    12121 aaagtcccgc tagtgccttg tccagttgca accacctgtt gatccgcatc acgcagttca
    12181 acgctgacat caccattggc caccacctgc cagtcaacag acgcgtggtt acagtcttgc
    12241 gcgacatgcg tcaccacggt gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt
    12301 acgctgcgat ggattccggc atagttaaag aaatcatgga agtaagactg ctttttcttg
    12361 ccgttttcgt cggtaatcac cattcccggc gggatagtct gccagttcag ttcgttgttc
    12421 acacaaacgg tgatacgtac acttttcccg gcaataacat acggcgtgac atcggcttca
    12481 aatggcgtat agccgccctg atgctccatc acttcctgat tattgaccca cactttgccg
    12541 taatgagtga ccgcatcgaa acgcagcacg atacgctggc ctgcccaacc tttcggtata
    12601 aagacttcgc gctgatacca gacgttgccc gcataattac gaatatctgc atcggcgaac
    12661 tgatcgttaa aactgcctgg cacagcaatt gcccggcttt cttgtaacgc gctttcccac
    12721 caacgctgat caattccaca gttttcgcga tccagactga atgcccacag gccgtcgagt
    12781 tttttgattt cacgggttgg ggtttctaca ggacggacga gtcgtcggtt ctgtaactat
    12841 catcatcatc atagacacac gaaataaagt aatcagatta tcagttaaag ctatgtaata
    12901 tttacaccat aaccaatcaa ttaaaaaata gatcagttta aagaaagatc aaagctcaaa
    12961 aaaataaaaa gagaaaaggg tcctaaccaa gaaaatgaag gagaaaaact agaaatttac
    13021 cctcagatct accatggtca agagtccccc gtgttctctc caaatgaaat gaacttcctt
    13081 atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg
    13141 agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
    13201 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttcaacga
    13261 tggcctttcc tttatcgcaa tgatggcatt tgtaggagcc accttccttt tccactatct
    13321 tcacaataaa gtgacagata gctgggcaat ggaatccgag gaggtttccg gatattaccc
    13381 tttgttgaaa agtctcaatt gccctttggt cttctgagac tgtatctttg atatttttgg
    13441 agtagacaag tgtgtcgtgc tccaccatgt tgacgaagat tttcttcttg tcattgagtc
    13501 gtaagagact ctgtatgaac tgttcgccag tctttacggc gagttctgtt aggtcctcta
    13561 tttgaatctt tgactccatg aagctaaact gaaggcggga aacgacaatc tgatccaagc
    13621 tcaagctgct ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    13681 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    13741 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttg
    13801 catgcctgca gttaattaag tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    13861 aggagatcct ttggatcaca aaaaaaaagc ccgctcatta ggcgggttaa attcccgggt
    13921 actgcagccg attcattaat gcaggttaac ctggcttatc gaaattaata cgactcacta
    13981 tagggagacc ggcagatctg atccctactc caaaaatgtc aaagatacag tctcagaaga
    14041 ccaaagggct attgagactt ttcaacaaag ggtaatttcg ggaaacctcc tcggattcca
    14101 ttgcccagct atctgtcact tcatcgaaag gacagtggaa aaggaaggtg gctcctacaa
    14161 atgccatcat tgcgataaag gaaaggctat cattcaagat gcctctgccg acagtggtcc
    14221 caaagatgga cccccaccca cgaggagcat cgtgggaaaa gaagacgttc caaccacgtc
    14281 ttcaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca
    14341 cccctactcc aaaaatgtca aagatactgt ctcagaagac caaagggcta t
//
