LOCUS       sequence-174373-       14812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disturb the expression of miR396 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14812)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM396
FEATURES             Location/Qualifiers
     source          1..14812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2359..2376)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2359..2375)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2368..2390)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2752..3097
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3114..3128,3319..5136)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          3129..3318
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             5143..5160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5195..5447
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5469..5493
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6794..7423
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7852..8925
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8991..9185
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9421..9443)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9529..9669
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9584..9603
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9684..9701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9855..10443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10530..11324)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11749..11773
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11851..12025
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12066..13091)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(13158..13834)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    14025..14046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14061..14066,14067..14084,14085..14091)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14099..14115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14104..14126
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14123..14139
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14123..14139
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14387..14406
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14387..14405
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttgttc
      361 aagaaagcta cctgtggaga gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatca gttcaagaac taagcctgtg gagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 cggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg tttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagatcatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgccca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaag aaaggcggac aggtatccgg taatctgctg cttgcaaact taattaactg
     2341 caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     2401 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     2461 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag
     2521 cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttagcttca tggagtcaaa
     2581 gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag
     2641 tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact
     2701 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
     2761 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
     2821 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
     2881 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     2941 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     3001 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
     3061 ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactcttg accatggtag
     3121 atctgagggt aaatttctag tttttctcct tcattttctt ggttaggacc cttttctctt
     3181 tttatttttt tgagctttga tctttcttta aactgatcta ttttttaatt gattggttat
     3241 ggtgtaaata ttacatagct ttaactgata atctgattac tttatttcgt gtgtctatga
     3301 tgatgatgat agttacagaa ccgacgactc gtccgtcctg tagaaacccc aacccgtgaa
     3361 atcaaaaaac tcgacggcct gtgggcattc agtctggatc gcgaaaactg tggaattgat
     3421 cagcgttggt gggaaagcgc gttacaagaa agccgggcaa ttgctgtgcc aggcagtttt
     3481 aacgatcagt tcgccgatgc agatattcgt aattatgcgg gcaacgtctg gtatcagcgc
     3541 gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg tgctgcgttt cgatgcggtc
     3601 actcattacg gcaaagtgtg ggtcaataat caggaagtga tggagcatca gggcggctat
     3661 acgccatttg aagccgatgt cacgccgtat gttattgccg ggaaaagtgt acgtatcacc
     3721 gtttgtgtga acaacgaact gaactggcag actatcccgc cgggaatggt gattaccgac
     3781 gaaaacggca agaaaaagca gtcttacttc catgatttct ttaactatgc cggaatccat
     3841 cgcagcgtaa tgctctacac cacgccgaac acctgggtgg acgatatcac cgtggtgacg
     3901 catgtcgcgc aagactgtaa ccacgcgtct gttgactggc aggtggtggc caatggtgat
     3961 gtcagcgttg aactgcgtga tgcggatcaa caggtggttg caactggaca aggcactagc
     4021 gggactttgc aagtggtgaa tccgcacctc tggcaaccgg gtgaaggtta tctctatgaa
     4081 ctgtgcgtca cagccaaaag ccagacagag tgtgatatct acccgcttcg cgtcggcatc
     4141 cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc acaaaccgtt ctactttact
     4201 ggctttggtc gtcatgaaga tgcggacttg cgtggcaaag gattcgataa cgtgctgatg
     4261 gtgcacgacc acgcattaat ggactggatt ggggccaact cctaccgtac ctcgcattac
     4321 ccttacgctg aagagatgct cgactgggca gatgaacatg gcatcgtggt gattgatgaa
     4381 actgctgctg tcggctttaa cctctcttta ggcattggtt tcgaagcggg caacaagccg
     4441 aaagaactgt acagcgaaga ggcagtcaac ggggaaactc agcaagcgca cttacaggcg
     4501 attaaagagc tgatagcgcg tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc
     4561 aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt tcgcgccact ggcggaagca
     4621 acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct
     4681 cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg
     4741 tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga acttctggcc
     4801 tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga tacgttagcc
     4861 gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc atggctggat
     4921 atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc
     4981 gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa agggatcttc
     5041 actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg gactggcatg
     5101 aacttcggtg aaaaaccgca gcagggaggc aaacaagcta gccaccacca ccaccaccac
     5161 gtgtgaatta caggtgacca gctcgaattt ccccgatcgt tcaaacattt ggcaataaag
     5221 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     5281 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     5341 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     5401 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattaaacta
     5461 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     5521 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     5581 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
     5641 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
     5701 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     5761 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     5821 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     5881 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     5941 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     6001 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     6061 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     6121 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
     6181 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
     6241 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
     6301 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
     6361 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
     6421 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     6481 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     6541 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
     6601 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
     6661 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     6721 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     6781 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     6841 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     6901 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     6961 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     7021 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     7081 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     7141 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     7201 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     7261 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
     7321 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
     7381 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
     7441 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
     7501 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
     7561 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
     7621 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
     7681 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
     7741 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
     7801 tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt
     7861 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     7921 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     7981 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     8041 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     8101 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     8161 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     8221 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     8281 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     8341 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     8401 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     8461 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     8521 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     8581 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     8641 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     8701 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     8761 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     8821 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     8881 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     8941 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc
     9001 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     9061 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     9121 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     9181 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     9241 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     9301 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     9361 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     9421 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     9481 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     9541 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     9601 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     9661 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9721 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9781 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9841 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9901 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9961 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10021 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10081 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10141 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10201 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10261 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    10321 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10381 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10441 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10501 taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat
    10561 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
    10621 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
    10681 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
    10741 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
    10801 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa
    10861 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
    10921 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
    10981 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
    11041 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
    11101 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
    11161 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
    11221 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
    11281 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
    11341 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
    11401 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
    11461 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
    11521 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
    11581 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
    11641 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
    11701 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
    11761 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
    11821 gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt
    11881 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
    11941 tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa
    12001 ctactcacac attattatgg agaaactcga gcttgtcgat cgacagatcc cggtcggcat
    12061 ctactctatt tctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
    12121 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
    12181 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
    12241 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
    12301 gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
    12361 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
    12421 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
    12481 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
    12541 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
    12601 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
    12661 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
    12721 gcagcgatcg catccatagc ctccgcgacc ggttgtagaa cagcgggcag ttcggtttca
    12781 ggcaggtctt gcaacgtgac accctgtgaa cggcgggaga tgcaataggt caggctctcg
    12841 ctaaactccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
    12901 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
    12961 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
    13021 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
    13081 ggctttttca tatctcattg ccccccggat ctgcgaaagc tcgagagaga tagatttgta
    13141 gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag
    13201 gaagggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca
    13261 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
    13321 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
    13381 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
    13441 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
    13501 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
    13561 agagtgtcgt gctccaccat gttcacatca atccacttgc tttgaagacg tggttggaac
    13621 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
    13681 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
    13741 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
    13801 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
    13861 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc
    13921 tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
    13981 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    14041 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    14101 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa ttcgagctcg
    14161 gtacccgggg atcctctagc tagattaatt aagaaaggcg gacaggtatc cggtaatctg
    14221 ctgcttgcaa acttaattaa gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    14281 aaggagatcc tttggatcac aaaaaaaaag cccgctcatt aggcgggtta aattcccggg
    14341 tactgcagcc gattcattaa tgcaggttaa cctggcttat cgaaattaat acgactcact
    14401 atagggagac cggcagatct gatccctact ccaaaaatgt caaagataca gtctcagaag
    14461 accaaagggc tattgagact tttcaacaaa gggtaatttc gggaaacctc ctcggattcc
    14521 attgcccagc tatctgtcac ttcatcgaaa ggacagtgga aaaggaaggt ggctcctaca
    14581 aatgccatca ttgcgataaa ggaaaggcta tcattcaaga tgcctctgcc gacagtggtc
    14641 ccaaagatgg acccccaccc acgaggagca tcgtgggaaa agaagacgtt ccaaccacgt
    14701 cttcaaagca agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc
    14761 acccctactc caaaaatgtc aaagatactg tctcagaaga ccaaagggct at
//
