LOCUS       sequence-174378-       14399 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disturb the expression of miR535 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14399)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM535
FEATURES             Location/Qualifiers
     source          1..14399
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    709..730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(745..750,751..768,769..775)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    783..799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     788..810
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     807..823
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     807..823
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1021..1040
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1021..1039
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1108..1453
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    1632..1808
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(2370..2388)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2371..2388)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(2459..3118)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKEITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3033..3052
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(3155..3160,3161..3177,3178..3183))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(3442..3459)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3442..3458)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3451..3473)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3835..4180
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(4197..4211,4402..6219)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          4212..4401
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             6226..6243
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      6278..6530
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6552..6576
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7877..8506
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8935..10008
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10074..10268
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10504..10526)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10612..10752
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10667..10686
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10767..10784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10938..11526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11613..12407)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12832..12856
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12934..13108
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(13149..14174)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(14249..14295)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
       61 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
      121 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
      181 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
      241 gacgagagtg tcgtgctcca ccatgttcac atcaatccac ttgctttgaa gacgtggttg
      301 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
      361 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
      421 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
      481 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
      541 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct
      601 gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      661 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      721 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      781 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaattcgag
      841 ctcggtaccc ggggatcctc tagctagatt aattaagttt gcaacagcag attacgcgca
      901 gaaaaaaagg atctcaagga gatcctttgg atcacaaaaa aaagcccgct cattaggcgg
      961 gttaaattcc cgggtactgc agccgattca ttaatgcagg ttaacctggc ttatcgaaat
     1021 taatacgact cactataggg agaccggtag atctgatccc tactccaaaa atgtcaaaga
     1081 tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa tttcgggaaa
     1141 cctcctcgga ttccattgcc cagctatctg tcacytcatc gaaaggacag trgaaaagga
     1201 aggtggctcc tacaaatgcc atcatygcga taaaggaaag gctatcattc aagatgcctc
     1261 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     1321 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg acataaggga
     1381 tgacgcacaa ccccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1441 tttggagagg acagcccaag cttggtaccg cgtgctctct ctactcgttg tcagttgttg
     1501 ttgttatggt ctaatttaaa tatggtctaa agaagaagaa tgcgtgctct ctctactcgt
     1561 tgtcaggatc cactagtgaa ttcggtacgc tgaaatcacc ggtctctctc tacaaatcta
     1621 tctctctcta ttttctccat aaataatgtg tgagtagttt cccgataagg gaaattaggg
     1681 ttcttatagg gtttcgctca tgtgttgagc atataagaga cccttagtat gtatttgtat
     1741 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa
     1801 tccagatctc ctaaagtccc tatagatctt tgtcgtgaat ataaaccaga cacgagacga
     1861 ctagacctgg agcccagacg ccgttcgaag ctagaagtac cgcttaggca ggaggccgtt
     1921 agggaaaaga tgctaaggca gggttggtta cgttgactcc cccgtaggtt tggtttaaat
     1981 atgatgaagt ggacggaagg aaggaggaag acaaggaagg ataaggttgc aggccctgtg
     2041 caaggtaaga agatggaaat ttgatagagg tacgctacta tacttatact atacgctaag
     2101 ggaatgattg tatttatacc ctataccccc taataacccc ttatcaattt aagaaataat
     2161 ccgcataagc ccccgcttaa aaattggtat cagagccatg aataggtcta tgaccaaaac
     2221 tcaagaggat aaaacctcac caaaatacga aagagtcctt aactctaaag ataaaagatc
     2281 tttcaagatc aaaactagtt ccctcacacc ggtgacgggg atcgcatgcg acctctagac
     2341 catcacattg gagcttcagc tgctcgagtt ctatagtgtc acctaaatcg tatgtgtatg
     2401 atacataagg ttatgtatta attgtagccg cgttctaacg tcgacaggcc tgtaccaatt
     2461 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     2521 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     2581 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     2641 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     2701 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     2761 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     2821 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatt accagctcac
     2881 cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca agaatgtgaa
     2941 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     3001 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     3061 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgatttct ttctccattt
     3121 tagcttcctt agctcctgaa aatctcgata actcattaaa acttatcgat gataagctgt
     3181 caaacatgag aatttagccc gcctaatgag cgggcttttt gctgtgatcc gcgttgctgg
     3241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tcccccctgg aagctccctc
     3361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tcttaattaa
     3421 ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3481 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3541 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta
     3601 gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttagct tcatggagtc
     3661 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
     3721 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
     3781 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
     3841 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
     3901 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
     3961 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     4021 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     4081 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     4141 ttcctctata taaggaagtt catttcattt ggagagaaca cgggggactc ttgaccatgg
     4201 tagatctgag ggtaaatttc tagtttttct ccttcatttt cttggttagg acccttttct
     4261 ctttttattt ttttgagctt tgatctttct ttaaactgat ctatttttta attgattggt
     4321 tatggtgtaa atattacata gctttaactg ataatctgat tactttattt cgtgtgtcta
     4381 tgatgatgat gatagttaca gaaccgacga ctcgtccgtc ctgtagaaac cccaacccgt
     4441 gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt
     4501 gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt
     4561 tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag
     4621 cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg
     4681 gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc
     4741 tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc
     4801 accgtttgtg tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc
     4861 gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccggaatc
     4921 catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg
     4981 acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt
     5041 gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact
     5101 agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat
     5161 gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc
     5221 atccggtcag tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt
     5281 actggctttg gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg
     5341 atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat
     5401 tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat
     5461 gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag
     5521 ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag
     5581 gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt
     5641 gccaacgaac cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa
     5701 gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac
     5761 gctcacaccg ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga
     5821 tggtatgtcc aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg
     5881 gcctggcagg agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta
     5941 gccgggctgc actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg
     6001 gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat
     6061 ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc
     6121 ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc
     6181 atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaag ctagccacca ccaccaccac
     6241 cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat cgttcaaaca tttggcaata
     6301 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     6361 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     6421 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     6481 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattaaa
     6541 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     6601 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     6661 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     6721 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     6781 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     6841 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     6901 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     6961 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     7021 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     7081 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     7141 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     7201 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     7261 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     7321 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     7381 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     7441 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     7501 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     7561 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     7621 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     7681 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     7741 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     7801 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     7861 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     7921 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     7981 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     8041 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     8101 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     8161 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     8221 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     8281 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     8341 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     8401 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     8461 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     8521 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     8581 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     8641 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     8701 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     8761 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     8821 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     8881 ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     8941 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     9001 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     9061 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     9121 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     9181 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     9241 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     9301 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     9361 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     9421 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     9481 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     9541 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     9601 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     9661 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     9721 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     9781 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     9841 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     9901 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     9961 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
    10021 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
    10081 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
    10141 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
    10201 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
    10261 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
    10321 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
    10381 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
    10441 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
    10501 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10561 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
    10621 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
    10681 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    10741 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10801 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10861 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10921 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10981 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    11041 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    11101 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    11161 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    11221 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    11281 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    11341 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    11401 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    11461 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    11521 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    11581 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
    11641 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    11701 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    11761 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    11821 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    11881 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    11941 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    12001 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    12061 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    12121 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    12181 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
    12241 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    12301 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    12361 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    12421 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    12481 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    12541 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
    12601 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
    12661 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
    12721 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
    12781 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
    12841 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
    12901 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg
    12961 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
    13021 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg
    13081 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tcccggtcgg
    13141 catctactct atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
    13201 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
    13261 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
    13321 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
    13381 tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
    13441 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
    13501 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
    13561 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
    13621 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
    13681 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
    13741 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
    13801 gccgcagcga tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt
    13861 tcaggcaggt cttgcaacgt gacaccctgt gaacggcggg agatgcaata ggtcaggctc
    13921 tcgctaaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
    13981 cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
    14041 ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
    14101 aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
    14161 tcaggctttt tcatatctca ttgccccccg gatctgcgaa agctcgagag agatagattt
    14221 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
    14281 gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata
    14341 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgct
//
