LOCUS       sequence-174379-       12669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  microRNA reporter relying on a bidirectional CAG-based promoter. 
            Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert 
            miRNA binding sites. Contains a Hygromycin selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12669)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors 
            in Embryonic Stem Cells.
  JOURNAL   PLoS One. 2016 May 6;11(5):e0155177. doi: 
            10.1371/journal.pone.0155177. eCollection 2016.
  PUBMED    27152616
REFERENCE   2  (bases 1 to 12669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-05-6"; volume: "11(5):e0155177. doi"; pages: " 
            10.1371/journal.pone.0155177. eCollection 2016"
COMMENT     Sequence Label: miReporter-CAG
FEATURES             Location/Qualifiers
     source          1..12669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    339..394
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     intron          591..1163
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             complement(join(1225..1935,1936..1938,1939..1941))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1267..1288)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1616..1635
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1875..1896
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(1948..2325)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     intron          2405..3421
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        complement(3422..3699)
                     /label=chicken beta-actin promoter
     enhancer        3709..4088
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        4096..4475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        4483..4862
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        4870..5249
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5251..5528
                     /label=chicken beta-actin promoter
     intron          5529..6545
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             6643..7020
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             7027..7734
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7172..7190)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7440..7459
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          7796..8368
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    8565..8620
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     polyA_signal    8957..9181
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(9188..10213)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(9188..10213)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10230..10729)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10278..10297)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     10671..10694
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      complement(10929..11517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11009..11028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11688..12479,12480..12548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12311..12330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12549..12653)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctcgacgca gtcgagttca taagagaaga gggacagcta tgactgggag tagtcaggag
       61 aggaggaaaa atctggctag taaaacatgt aaggaaaatt ttagggatgt taaagaaaaa
      121 aataacacaa aacaaaatat aaaaaaaatc taacctcaag tcaaggcttt tctatggaat
      181 aaggaatgga cagcaggggg ctgtttcata tactgatgac ctctttatag ccaacctttg
      241 ttcatggcag ccagcatatg ggcatatgtt gccaaactct aaaccaaata ctcattctga
      301 tgttttaaat gatttgccct cccatatgtc cttccgagtg agagacacaa aaaattccaa
      361 cacactattg caatgaaaat aaatttcctt tattagccag aagtcagatg ctcaaggggc
      421 ttcatgatgt ccccataatt tttggcagag ggaaaaagat ctcagtggta tttgtgagcc
      481 agggcattgg ccacaccagc caccaccttc tgataggcag cctgcacctg aggagtgaat
      541 tctttgccaa aatgatgaga cagcacaaca accagcacgt tgcccaggag ctgtaggaaa
      601 gagaagaagg catgaacatg gttagcagag gggcccggtt tggactcaga gtattttatc
      661 ctcatctcaa acagtgtata tcattgtaac cataaagaga aaggcaggat gatgaccagg
      721 gtgtagttgt ttctaccaat aagaatattt ccacgccagc cagaatttat atgcagaaat
      781 attctacctt atcatttaat tataacaatt gttctctaaa actgtgctga agtacaatat
      841 aatataccct gattgccttg aaaaaaaaga gattagagaa agtacttaca atctgacaaa
      901 taaacaaaag tgaatttaaa aattcgttac aaatgcaagc taaagtttaa cgaaaaagtt
      961 acagaaaatg aaaagaaaat aagaggagac aatggttgtc aacagagtag aaagtgaaag
     1021 aaacaaaatt atcatgaggg tccatggtga tacaagggac atcttcccat tctaaacaac
     1081 accctgaaaa ctttgccccc tccatataac atgaatttta caatagcgaa aaagaaagaa
     1141 caatcaaggg tccccaaact caccctgaag ttctcagtct agctcgacgg atccagatct
     1201 gctagcctcg actcatggat ctgtcttgta cagctcgtcc atgccgagag tgatcccggc
     1261 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
     1321 ggcggactgg tagctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
     1381 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
     1441 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
     1501 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
     1561 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
     1621 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
     1681 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc acatcaggcc
     1741 gtagccgaag gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
     1801 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
     1861 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
     1921 ctcctcgccc ttgctcacca tctcgacctt agcgctggtg tacttggtga tggccttagt
     1981 accctcggac acggcgtgct tggccaactc cccaggcagc agcaggcgca cggccgtctg
     2041 gatctccctg gaggtgatgg tcgagcgctt gttgtaatgc gccaggcggg aagcctcacc
     2101 tgcgatgcgc tcgaaaatgt cgttcacaaa cgaattcatg atgcccatgg ccttggacga
     2161 aatgccggtg tcagggtgga cctgcttcag aaccttgtac acatagatgg aatagctctc
     2221 cttgcggctg cgcttgcgct tcttgccgcc tttcttctgc gccttagtca ccgccttctt
     2281 ggagcccttt ttcggggcgg gagcagactt cgctggctct ggcatggtgg cgactcgaaa
     2341 gcttgtcgac gaattctttg ccaaaatgat gagacagcac aataaccagc acgttgccca
     2401 ggagctgtag gaaaaagaag aaggcatgaa catggttagc agaggctcta gagccgccgg
     2461 tcacacgcca gaagccgaac cccgccctgc cccgtccccc ccgaaggcag ccgtcccccc
     2521 gcggacagcc ccgaggctgg agagggagaa ggggacggcg gcgcggcgac gcacgaaggc
     2581 cctccccgcc catttccttc ctgccggcgc cgcaccgctt cgccccgcgc ccgctagagg
     2641 gggtgcggcg gcgcctccca gatttcggct ccgcacagat ttgggacaaa ggaagtccct
     2701 gcgccctctc gcacgattac cataaaaggc aatggctgcg gctcgccgcg cctcgacagc
     2761 cgccggcgct ccgggggccg ccgcgcccct cccccgagcc ctccccggcc cgaggcggcc
     2821 ccgccccgcc cggcaccccc acctgccgcc accccccgcc cggcacggcg agccccgcgc
     2881 cacgccccgt acggagcccc gcacccgaag ccgggccgtg ctcagcaact cggggagggg
     2941 ggtgcagggg gggttgcagc ccgaccgacg cgcccacacc ccctgctcac ccccccacgc
     3001 acacaccccg cacgcagcct ttgttcccct cgcagccccc cccgcaccgc ggggcaccgc
     3061 ccccggccgc gctcccctcg cgcacactgc ggagcgcaca aagccccgcg ccgcgcccgc
     3121 agcgctcaca gccgccgggc agcgcggagc cgcacgcggc gctccccacg cacacacaca
     3181 cgcacgcacc ccccgagccg ctccccccgc acaaagggcc ctcccggagc ccctcaaggc
     3241 tttcacgcag ccacagaaaa gaaacaagcc gtcattaaac caagcgctaa ttacagcccg
     3301 gaggagaagg gccgtcccgc ccgctcacct gtgggagtaa cgcggtcagt cagagccggg
     3361 gcgggcggcg cgaggcggcg gcggagcggg gcacggggcg aaggcagcgc gcagcgactc
     3421 ccgcccgccg cgcgcttcgc tttttatagg gccgccgccg ccgccgcctc gccataaaag
     3481 gaaactttcg gagcgcgccg ctctgattgg ctgccgccgc acctctccgc ctcgccccgc
     3541 cccgcccctc gccccgcccc gccccgcctg gcgcgcgccc cccccccccc cccgccccca
     3601 tcgctgcaca aaataattaa aaaataaata aatacaaaat tgggggtggg gagggggggg
     3661 agatggggag agtgaagcag aacgtggggc tcacctcgac catggctcga cattgattat
     3721 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     3781 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3841 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3901 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3961 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     4021 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     4081 ttaccatggg ttctcgacat tgattattga ctagttatta atagtaatca attacggggt
     4141 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     4201 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     4261 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     4321 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     4381 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     4441 agtacatcta cgtattagtc atcgctatta ccatgggttc tcgacattga ttattgacta
     4501 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     4561 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     4621 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     4681 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     4741 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     4801 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     4861 tgggttctcg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     4921 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     4981 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     5041 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     5101 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     5161 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     5221 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
     5281 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
     5341 gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg
     5401 aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
     5461 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
     5521 ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc
     5581 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc
     5641 cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc
     5701 tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg
     5761 gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct
     5821 gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg
     5881 cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc
     5941 ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc
     6001 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta
     6061 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
     6121 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga
     6181 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
     6241 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc
     6301 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg
     6361 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg
     6421 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct
     6481 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
     6541 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcg
     6601 ttccatggct cgaggttacg cgtaagcttt cgagtcgcca ccatgccaga gccagcgaag
     6661 tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc
     6721 ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg
     6781 aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat gaattcgttt
     6841 gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag
     6901 cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag
     6961 ttggccaagc acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag
     7021 gtcgagatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     7081 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     7141 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     7201 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     7261 aagcaccccg ccgacattcc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     7321 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     7381 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     7441 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     7501 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     7561 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     7621 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     7681 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtctaca
     7741 gattgagtcg agtctagaag atcttgatca gtcgagctag actgagaact tcagggtgag
     7801 tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt tatatggagg
     7861 gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc accatggacc
     7921 ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat
     7981 tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatttt
     8041 taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatctc ttttttttca
     8101 aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat
     8161 taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat
     8221 tggtagaaac aactacaccc tggtcatcat cctgcctttc tctttatggt tacaatgata
     8281 tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca
     8341 tgttcatgcc ttcttctctt tcctacagct cctgggcaac gtgctggttg ttgtgctgtc
     8401 tcatcatttt ggcaaagaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     8461 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     8521 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     8581 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     8641 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     8701 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     8761 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     8821 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     8881 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     8941 aactcgactg cgtcgaccat agagcccacc gcatccccag catgcctgct attgtcttcc
     9001 caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc
     9061 agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc
     9121 agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca
     9181 gctcgaccta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     9241 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     9301 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     9361 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     9421 acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     9481 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     9541 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     9601 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     9661 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     9721 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     9781 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     9841 ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt
     9901 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
     9961 cgctgaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
    10021 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
    10081 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
    10141 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
    10201 caggcttttt catggtggcg actcgacgtc gaaaggcccg gagatgagga agaggagaac
    10261 agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg
    10321 gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc ccttcccagc
    10381 ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca aaggcctacc
    10441 cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg tgctacttcc
    10501 atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct gactagggga
    10561 ggagtagaag gtggcgcgaa ggggccacca aagaacggag ccggttggcg cctaccggtg
    10621 gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc cagcggggct
    10681 gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg gtagactcga
    10741 gttacgcgtt tgatatcatg gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    10801 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    10861 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    10921 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    10981 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    11041 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    11101 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    11161 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    11221 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    11281 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    11341 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    11401 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    11461 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    11521 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    11581 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    11641 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    11701 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    11761 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    11821 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    11881 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    11941 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    12001 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    12061 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    12121 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    12181 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    12241 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    12301 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    12361 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    12421 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    12481 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    12541 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    12601 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    12661 ccccgaatt
//
