LOCUS       sequence-174380-       12640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  microRNA reporter relying on a bidirectional CAG-based promoter. 
            Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert 
            miRNA binding sites. Contains a Hygromycin selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12640)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors 
            in Embryonic Stem Cells.
  JOURNAL   PLoS One. 2016 May 6;11(5):e0155177. doi: 
            10.1371/journal.pone.0155177. eCollection 2016.
  PUBMED    27152616
REFERENCE   2  (bases 1 to 12640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-05-6"; volume: "11(5):e0155177. doi"; pages: " 
            10.1371/journal.pone.0155177. eCollection 2016"
COMMENT     Sequence Label: miReporter-CAG
FEATURES             Location/Qualifiers
     source          1..12640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             509..886
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             893..1600
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1038..1056)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1306..1325
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          1662..2234
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    2431..2486
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     polyA_signal    2823..3047
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3054..4079)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(3054..4079)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4096..4595)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4144..4163)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4537..4560
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      complement(4795..5383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4875..4894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5554..6345,6346..6414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6177..6196
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6415..6519)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6874..6929
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     intron          7126..7698
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             complement(join(7760..8470,8471..8473,8474..8476))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7802..7823)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8151..8170
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8410..8431
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(8483..8860)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     intron          8940..9956
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        complement(9957..10234)
                     /label=chicken beta-actin promoter
     enhancer        10244..10623
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        10631..11010
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        11018..11397
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        11405..11784
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11786..12063
                     /label=chicken beta-actin promoter
ORIGIN
        1 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
       61 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
      121 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
      181 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
      241 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
      301 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
      361 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
      421 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcgttcc atggctcgag
      481 gttacgcgta agctttcgag tcgccaccat gccagagcca gcgaagtctg ctcccgcccc
      541 gaaaaagggc tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa
      601 gcgcagccgc aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc
      661 tgacaccggc atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt
      721 cgagcgcatc gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac
      781 ctccagggag atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc
      841 cgtgtccgag ggtactaagg ccatcaccaa gtacaccagc gctaaggtcg agatggtgag
      901 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
      961 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     1021 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     1081 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     1141 cattcccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     1201 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     1261 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     1321 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     1381 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     1441 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     1501 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     1561 gggccgccac tccaccggcg gcatggacga gctgtacaag tctacagatt gagtcgagtc
     1621 tagaagatct tgatcagtcg agctagactg agaacttcag ggtgagtttg gggacccttg
     1681 attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca
     1741 gggtgttgtt tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt
     1801 gtttctttca ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt
     1861 ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg
     1921 tttatttgtc agattgtaag tactttctct aatctctttt ttttcaaggc aatcagggta
     1981 tattatattg tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta
     2041 gaatatttct gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact
     2101 acaccctggt catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga
     2161 tgaggataaa atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct
     2221 tctctttcct acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca
     2281 aagaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
     2341 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca
     2401 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     2461 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     2521 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     2581 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     2641 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     2701 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     2761 tcctctcctg actactccca gtcatagctg tccctcttct cttatgaact cgactgcgtc
     2821 gaccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt
     2881 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
     2941 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
     3001 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagctc gacctattcc
     3061 tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag
     3121 ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct
     3181 ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg
     3241 tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc
     3301 ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa
     3361 gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc
     3421 gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag
     3481 ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc
     3541 tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga
     3601 tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt
     3661 ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca
     3721 tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc
     3781 aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca
     3841 atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga
     3901 tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca
     3961 tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg
     4021 tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcatg
     4081 gtggcgactc gacgtcgaaa ggcccggaga tgaggaagag gagaacagcg cggcagacgt
     4141 gcgcttttga agcgtgcaga atgccgggcc tccggaggac cttcgggcgc ccgccccgcc
     4201 cctgagcccg cccctgagcc cgcccccgga cccacccctt cccagcctct gagcccagaa
     4261 agcgaaggag caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc
     4321 agcggtgctg tccatctgca cgagactagt gagacgtgct acttccattt gtcacgtcct
     4381 gcacgacgcg agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg
     4441 cgcgaagggg ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt
     4501 gcgaggccag aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc
     4561 tccagactgc cttgggaaaa gcgcctcccc tacccggtag actcgagtta cgcgtttgat
     4621 atcatggctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4681 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4741 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4801 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4861 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4921 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4981 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5041 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5101 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5161 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5221 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5281 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5341 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5401 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5461 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5521 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5581 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5641 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5701 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5761 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5821 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5881 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5941 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6001 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6061 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6121 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6181 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6241 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6301 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6361 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6421 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6481 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaattgctcg
     6541 acgcagtcga gttcataaga gaagagggac agctatgact gggagtagtc aggagaggag
     6601 gaaaaatctg gctagtaaaa catgtaagga aaattttagg gatgttaaag aaaaaaataa
     6661 cacaaaacaa aatataaaaa aaatctaacc tcaagtcaag gcttttctat ggaataagga
     6721 atggacagca gggggctgtt tcatatactg atgacctctt tatagccaac ctttgttcat
     6781 ggcagccagc atatgggcat atgttgccaa actctaaacc aaatactcat tctgatgttt
     6841 taaatgattt gccctcccat atgtccttcc gagtgagaga cacaaaaaat tccaacacac
     6901 tattgcaatg aaaataaatt tcctttatta gccagaagtc agatgctcaa ggggcttcat
     6961 gatgtcccca taatttttgg cagagggaaa aagatctcag tggtatttgt gagccagggc
     7021 attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattcttt
     7081 gccaaaatga tgagacagca caacaaccag cacgttgccc aggagctgta ggaaagagaa
     7141 gaaggcatga acatggttag cagaggggcc cggtttggac tcagagtatt ttatcctcat
     7201 ctcaaacagt gtatatcatt gtaaccataa agagaaaggc aggatgatga ccagggtgta
     7261 gttgtttcta ccaataagaa tatttccacg ccagccagaa tttatatgca gaaatattct
     7321 accttatcat ttaattataa caattgttct ctaaaactgt gctgaagtac aatataatat
     7381 accctgattg ccttgaaaaa aaagagatta gagaaagtac ttacaatctg acaaataaac
     7441 aaaagtgaat ttaaaaattc gttacaaatg caagctaaag tttaacgaaa aagttacaga
     7501 aaatgaaaag aaaataagag gagacaatgg ttgtcaacag agtagaaagt gaaagaaaca
     7561 aaattatcat gagggtccat ggtgatacaa gggacatctt cccattctaa acaacaccct
     7621 gaaaactttg ccccctccat ataacatgaa ttttacaata gcgaaaaaga aagaacaatc
     7681 aagggtcccc aaactcaccc tgaagttctc agtctagctc gacggatcca gatctgctag
     7741 cctcgactca tggatctgtc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     7801 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
     7861 actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     7921 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     7981 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
     8041 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     8101 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     8161 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     8221 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcacatc aggccgtagc
     8281 cgaaggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
     8341 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
     8401 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
     8461 cgcccttgct caccatctcg accttagcgc tggtgtactt ggtgatggcc ttagtaccct
     8521 cggacacggc gtgcttggcc aactccccag gcagcagcag gcgcacggcc gtctggatct
     8581 ccctggaggt gatggtcgag cgcttgttgt aatgcgccag gcgggaagcc tcacctgcga
     8641 tgcgctcgaa aatgtcgttc acaaacgaat tcatgatgcc catggccttg gacgaaatgc
     8701 cggtgtcagg gtggacctgc ttcagaacct tgtacacata gatggaatag ctctccttgc
     8761 ggctgcgctt gcgcttcttg ccgcctttct tctgcgcctt agtcaccgcc ttcttggagc
     8821 cctttttcgg ggcgggagca gacttcgctg gctctggcat ggtggcgact cgaaagcttg
     8881 tcgacgaatt ctttgccaaa atgatgagac agcacaataa ccagcacgtt gcccaggagc
     8941 tgtaggaaaa agaagaaggc atgaacatgg ttagcagagg ctctagagcc gccggtcaca
     9001 cgccagaagc cgaaccccgc cctgccccgt cccccccgaa ggcagccgtc cccccgcgga
     9061 cagccccgag gctggagagg gagaagggga cggcggcgcg gcgacgcacg aaggccctcc
     9121 ccgcccattt ccttcctgcc ggcgccgcac cgcttcgccc cgcgcccgct agagggggtg
     9181 cggcggcgcc tcccagattt cggctccgca cagatttggg acaaaggaag tccctgcgcc
     9241 ctctcgcacg attaccataa aaggcaatgg ctgcggctcg ccgcgcctcg acagccgccg
     9301 gcgctccggg ggccgccgcg cccctccccc gagccctccc cggcccgagg cggccccgcc
     9361 ccgcccggca cccccacctg ccgccacccc ccgcccggca cggcgagccc cgcgccacgc
     9421 cccgtacgga gccccgcacc cgaagccggg ccgtgctcag caactcgggg aggggggtgc
     9481 agggggggtt gcagcccgac cgacgcgccc acaccccctg ctcacccccc cacgcacaca
     9541 ccccgcacgc agcctttgtt cccctcgcag ccccccccgc accgcggggc accgcccccg
     9601 gccgcgctcc cctcgcgcac actgcggagc gcacaaagcc ccgcgccgcg cccgcagcgc
     9661 tcacagccgc cgggcagcgc ggagccgcac gcggcgctcc ccacgcacac acacacgcac
     9721 gcaccccccg agccgctccc cccgcacaaa gggccctccc ggagcccctc aaggctttca
     9781 cgcagccaca gaaaagaaac aagccgtcat taaaccaagc gctaattaca gcccggagga
     9841 gaagggccgt cccgcccgct cacctgtggg agtaacgcgg tcagtcagag ccggggcggg
     9901 cggcgcgagg cggcggcgga gcggggcacg gggcgaaggc agcgcgcagc gactcccgcc
     9961 cgccgcgcgc ttcgcttttt atagggccgc cgccgccgcc gcctcgccat aaaaggaaac
    10021 tttcggagcg cgccgctctg attggctgcc gccgcacctc tccgcctcgc cccgccccgc
    10081 ccctcgcccc gccccgcccc gcctggcgcg cgcccccccc ccccccccgc ccccatcgct
    10141 gcacaaaata attaaaaaat aaataaatac aaaattgggg gtggggaggg gggggagatg
    10201 gggagagtga agcagaacgt ggggctcacc tcgaccatgg ctcgacattg attattgact
    10261 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
    10321 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
    10381 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
    10441 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
    10501 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
    10561 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
    10621 atgggttctc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
    10681 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
    10741 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
    10801 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
    10861 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
    10921 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
    10981 atctacgtat tagtcatcgc tattaccatg ggttctcgac attgattatt gactagttat
    11041 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
    11101 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
    11161 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
    11221 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
    11281 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
    11341 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgggt
    11401 tctcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
    11461 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
    11521 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
    11581 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
    11641 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
    11701 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
    11761 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
    11821 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
    11881 gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
    11941 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
    12001 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
    12061 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
    12121 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
    12181 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
    12241 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
    12301 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
    12361 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
    12421 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
    12481 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
    12541 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
    12601 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
//
