LOCUS       sequence-174383-        6078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hybrid McpC[1-350]-YSYEH-Tar[272-553].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Bi S, Pollard AM, Yang Y, Jin F, Sourjik V
  TITLE     Engineering Hybrid Chemotaxis Receptors in Bacteria.
  JOURNAL   ACS Synth Biol. 2016 Jun 10.
  PUBMED    27285081
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jun 10."
COMMENT     Sequence Label: pSB6
FEATURES             Location/Qualifiers
     source          1..6078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      341..375
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(794..1453)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1368..1387
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1454..1556)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1948..1965)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2082..2627)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2162..2181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaacagagcc gtggcatcga tcaagtcgca ttggcggttt cggaaatgga tcgcgtcacg
       61 caacagaacg catcgctggt gcaggaatca gctgccgccg ccgctgcgct ggaagaacag
      121 gcgagtcgtt taacgcaagc agtttccgcg ttccgtctgg cagccagccc actcaccaat
      181 aaaccgcaaa caccatcccg tcctgccagt gagcaaccac cggctcagcc acgactgcga
      241 attgctgaac aagatccaaa ctgggaaaca ttttgaggat ccgcggataa ataagtaacg
      301 atccggtcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
      361 ccgggcgttt tttattggtg agaatcgcag caacttgtcg cgccaatcga gccatgtcgt
      421 cgtcaacgac cccccattca agaacagcaa gcagcattga gaactttgga atccagtccc
      481 tcttccacct gctgaccgga tcagcagtcc ccggaacatc gtagctgacg ccttcgcgtt
      541 gctcagttgt ccaaccccgg aaacgggaaa aagcaagttt tccccgctcc cggcgtttca
      601 ataactgaaa accatactat ttcacagttt aaatcacatt aaacgacagt aatccccgtt
      661 gatttgtgcg ccaacacaga tccgtcgacc gggtcgaatt tgctttcgaa tttctgccat
      721 tcatccgctt attatcactt attcaggcgt agcaaccagg cgtttaaggg caccaataac
      781 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
      841 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
      901 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
      961 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     1021 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1081 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1141 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1201 atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc
     1261 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1321 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1381 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1441 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1501 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     1561 ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta
     1621 tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg
     1681 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct
     1741 gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc
     1801 accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg
     1861 gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa
     1921 tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     1981 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa
     2041 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc
     2101 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
     2161 taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
     2221 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
     2281 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     2341 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
     2401 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
     2461 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
     2521 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
     2581 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
     2641 ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg
     2701 aagtcagccc catacgatat aagttgtaat tctcatgttt gacagcttat catcgatccc
     2761 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
     2821 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
     2881 aatttaactg tgataaacta ccgcattaaa gcttcttgcg aaacagctct cgaccaggtt
     2941 tatactggtc gtgcagatga aagcagtttt tgccgaggtc gattccgacc agcgccatat
     3001 cgctcatagt gatggtctcc gaattataaa caccctgcga aagcgtagcc tacgcagggt
     3061 gtcgggtgct catcccatta cccgttttcc catctcttaa cttaaggggg gaacggtgtc
     3121 ctgtctttca tggggctcgt tcactgctaa cttacctgct tccctgtctg tcaattctct
     3181 ctatcctgcg atcccgcgaa gaaccaaaaa agctcgacag agggcgcggt cattttaggt
     3241 cgggcggatc ggcgccgccg gctcggctgg tgtgccgcac agcaccgcct acgtgagctg
     3301 ccagttgatg aacttccccc gttgccagct agggcgcaag cgggctgtat aagatcactg
     3361 cccatcacat tgatcggctc ggattttttc tcaatccgta aacaggtcaa acatcagttg
     3421 ccgcaaccaa atattggcta ggtccttgtg gtacttcgca tgccagaaca tgttgatggc
     3481 tatttcaggc aagacgactg ggtgcggcaa ggcgcttagg ccgaagggct ccacacagca
     3541 gtcggctaaa cgtatcggca cagtggcgag cagatcggtg cgctggagga tgtggccaac
     3601 ggcggcgaag tgcggcactt ccagacggat gtcgcgccgg atgccgaccc gtgtcatgta
     3661 cgtgtccacc tcgccgtggc cggtgccagc ggcgatgaca cgcacgtggc cgtaggaaca
     3721 gaagcgctcc agagtcaggg gttcgcgggt gactggatgg tccttgcgac ataggcacac
     3781 gtagtgattc tggagcagcc ggcgctgaaa gaagccagtt tgcagattgg gaagcaggcc
     3841 cacggccaag tccacggttc cgttctgcaa ggcctgcatc aggctcatcg aactgtcgcg
     3901 caccgtactg atcacgcaat tgggggcctg gtgagccagc acatccatca gccgcggcat
     3961 gaagtagatc tcgccaatgt cggtcatggc cagggtgaag gtacgctcgc tggtcagcgg
     4021 atcgaagctt tcatggtgct gtagggcgtt gcgcagtgcg tgcatggccg aagtgacggg
     4081 ctcggccaga tgcgcggcat agggtgtggg ttccattccc tgatgtgtgc gcacgaagag
     4141 tgggtcctgt agcgaggtgc gcaggcgttt cagcgcattg ctcacggcag gctgggtcag
     4201 gcccaggttc tccgcagtga tagagacgcg tctgtcgacc agcaactggt tgaacaccac
     4261 cagcaggttt aaatccaggt cacgcagttc catggggcct cgcttgggtt attgctggtg
     4321 cccggccggg cgcaatattc atgttgatga tttattatat atcgagtggt gtatttatca
     4381 atattgtttg ctccgttatc gttattaaca agtcatcaat aatgccataa ggagtaccat
     4441 atgtttaaaa aacttcacat gaagattgct gtttttgttt cgattatgtt aatcatcaca
     4501 gttgttctct taatgctgtc ttcttatttg acgctaaaac cgatgattac agaggatggg
     4561 aaaaatacaa ctcaaaacgt gacgcagtca cttgaacaaa atattgaatt gcaattaaag
     4621 agttatgcca tttcgttatc aagattggct aatggggaat taacgcatac atttgtaacg
     4681 aaaccaagta aagaagcttc tcggctgttt cacgatgaca ttaagcaaat caaagacaac
     4741 gatgattatg ttgcgatggc ttacataggc accgcaaaaa aagaaatgtt tacatatcca
     4801 aaagcggatt tcgctgagga ttacgatcca acatcaagac catggtataa gcttgcagct
     4861 gaaaccccgg atcaagttgt ttggacggaa ccttataaag atgtcgtgac aggagacatg
     4921 attgttactg catcgaaagc gatcttagac aggcaaaaag tgataggggt tgccagctat
     4981 gacttgaaac tctctgctat ccagagtatg gtcaataaac aaaaggtgcc ttacaaagga
     5041 tttgcctttt tagctgatgc gagcggcaat cttttagccc atccgtcaaa tcaaggaaaa
     5101 aatatatcaa aggatcaaac actgcaaacc atcgcatccg agaaaaaagg gatacaagat
     5161 gtgaatggga aaatggttgt gtaccaaacg attggcgaga caggctggaa ggtcggaacg
     5221 caatttgata ccgatcaatt aatgtggatt tcagataaaa tgaatcgggc caatctttgg
     5281 atatcattga ttgcgcttat cataacaatt attctcagct acttcctggc caaaacgata
     5341 accggtccga tacagcagct gatcgtaaaa acgaaagctg tttccgcagg cgatttaaca
     5401 gtgcgcgcgg aatcaaaatc taaggatgaa gtcggtattt taacaagaga tttcaatctg
     5461 atggttgaaa atatgaagga aatggttgag tattcctacg agcactcaga tgccatctat
     5521 gccggtaccc gtgaaattgc ggcgggcaac accgatcttt cctcccgtac tgaacagcag
     5581 gcatccgcgc tggaagaaac tgccgccagc atggagcagc tcaccgcgac agtgaagcaa
     5641 aacgccgata acgcccgcca ggcctcgcaa ctggcgcaaa gtgcctccga caccgcccag
     5701 cacggcggca aagtggtgga tggcgtagtg aaaacgatgc atgagatcgc cgatagttcg
     5761 aagaaaattg ccgacattat cagcgttatc gacggtattg ccttccagac taatatcctc
     5821 gcgctgaatg ccgcggttga agccgcgcgt gcgggtgaac agggccgtgg ttttgccgtg
     5881 gtggcgggtg aagtgcgtaa tcttgccagt cgcagcgccc aggcggcaaa agagatcaaa
     5941 gccctcattg aagactccgt ctcacgcgtt gataccggtt cggtgctggt cgaaagcgcc
     6001 ggggaaacaa tgaacaatat cgtcaatgct gtcactcgcg tgactgacat tatgggcgag
     6061 attgcatcgg catcggat
//
