LOCUS       sequence-17439-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Chin L, Tam A, Pomerantz J, Wong M, Holash J, Bardeesy N, Shen Q, 
            O'Hagan R, Pantginis J, Zhou H, Horner JW, Cordon-Cardo C, 
            Yancopoulos GD, DePinho RA
  TITLE     Essential role for oncogenic Ras in tumour maintenance.
  JOURNAL   Nature. 1999 Jul 29. 400(6743):468-72.
  PUBMED    10440378
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1999 Jul 29. 400(6743):468-72."
COMMENT     Sequence Label: tet operator
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(84..108)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(105..143)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    176..194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    218..236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    302..320
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    344..362
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    386..404
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(422..438)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(422..438)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(470..489)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(471..489)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(496..513)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(496..512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(505..527)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(741..760)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgccaattc ggggccgcgg aaggctggat cggtcccggt gtcttctatg gaggtcaaaa
       61 cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct gcttatatag
      121 gcctcccacc gtacacgcct acctcgaccc gggtaccgag ctcgactttc acttttctct
      181 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
      241 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
      301 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
      361 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgaggg
      421 ggatccacta gttctagagc ggccgccacc gcggtggagc tccaattcgc cctatagtga
      481 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      541 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      601 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
      661 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      721 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      781 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
      841 acggcacctc gaccccaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
      901 tgaatagacg
//
