LOCUS       sequence-174390-        3953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Apr 5. 108(14):5632-7."
COMMENT     Sequence Label: pEntr-Tbx5flbio
FEATURES             Location/Qualifiers
     source          1..3953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2057..2080
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2093..2137
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     protein_bind    complement(2182..2281)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2393..2412)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2404..3213
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2471..2490)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3303..3891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3792..3811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg
      481 acgccgccgc gatcgcatgg ccgacgcaga cgagggcttt ggcctggcgc acacgcctct
      541 ggagcctgac gcaaaagacc tgccctgcga ttcgaaaccc gagagcgcgc tcggggcccc
      601 cagcaagtcc ccgtcgtccc cgcaggccgc cttcacccag cagggcatgg agggaatcaa
      661 agtgtttctc catgaaagag aactgtggct aaaattccac gaagtgggca cggaaatgat
      721 cataaccaag gctggaaggc ggatgtttcc cagttacaaa gtgaaggtga cgggccttaa
      781 tcccaaaacg aagtacattc ttctcatgga cattgtacct gccgacgatc acagatacaa
      841 attcgcagat aataaatggt ctgtgacggg caaagctgag cccgccatgc ctggccgcct
      901 gtacgtgcac ccagactccc ccgccaccgg ggcgcattgg atgaggcagc tcgtctcctt
      961 ccagaaactc aagctcacca acaaccacct ggacccattt gggcatatta ttctaaattc
     1021 catgcacaaa taccagccta gattacacat cgtgaaagcg gatgaaaata atggatttgg
     1081 ctcaaaaaat acagcgttct gcactcacgt ctttcctgag actgcgttta tagcagtgac
     1141 ttcctaccag aaccacaaga tcacgcaatt aaagattgag aataatccct ttgccaaagg
     1201 atttcggggc agtgatgaca tggagctgca cagaatgtca agaatgcaaa gtaaagaata
     1261 tcccgtggtc cccaggagca ccgtgaggca aaaagtggcc tccaaccaca gtcctttcag
     1321 cagcgagtct cgagctctct ccacctcatc caatttgggg tcccaatacc agtgtgagaa
     1381 tggtgtttcc ggcccctccc aggacctcct gcctccaccc aacccatacc cactgcccca
     1441 ggagcatagc caaatttacc attgtaccaa gaggaaagag gaagaatgtt ccaccacaga
     1501 ccatccctat aagaagccct acatggagac atcacccagt gaagaagatt ccttctaccg
     1561 ctctagctat ccacagcagc agggcctggg tgcctcctac aggacagagt cggcacagcg
     1621 gcaagcttgc atgtatgcca gctctgcgcc ccccagcgag cctgtgccca gcctagagga
     1681 catcagctgc aacacgtggc caagcatgcc ttcctacagc agctgcaccg tcaccaccgt
     1741 gcagcccatg gacaggctac cctaccagca cttctccgct cacttcacct cggggcccct
     1801 ggtccctcgg ctggctggca tggccaacca tggctcccca cagctgggag agggaatgtt
     1861 ccagcaccag acctccgtgg cccaccagcc tgtggtcagg cagtgtgggc ctcagactgg
     1921 cctgcagtcc cctggcaccc ttcagccccc tgagttcctc tactctcatg gcgtgccaag
     1981 gactctatcc cctcatcagt accactctgt gcacggagtt ggcatggtgc cagagtggag
     2041 cgacaatagc acgcgtgact acaaagacga tgacgataaa ggtgatgggt ccggcctgaa
     2101 cgacatcttc gaggctcaga aaatcgaatg gcacgaaggc gcgccgagct cgaggtaggc
     2161 ggccgcactc gagatatcta gacccagctt tcttgtacaa agttggcatt ataagaaagc
     2221 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     2281 gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2341 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2401 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2461 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2521 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2581 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2641 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2701 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2761 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2821 aacagcgatc gtgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2881 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2941 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     3001 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     3061 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3121 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3181 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat
     3241 tattcagatt gggccccgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3301 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3361 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3421 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3481 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3541 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3601 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3661 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3721 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3781 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3841 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3901 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtt
//
