LOCUS       sequence-174392-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Long stokes-shift variant of red fluorescent protein mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Shen Y, Chen Y, Wu J, Shaner NC, Campbell RE
  TITLE     Engineering of mCherry variants with long Stokes shift, red-shifted 
            fluorescence, and low cytotoxicity.
  JOURNAL   PLoS One. 2017 Feb 27;12(2):e0171257. doi: 
            10.1371/journal.pone.0171257. eCollection 2017.
  PUBMED    28241009
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-02-27"; volume: "12(2):e0171257. doi"; pages: " 
            10.1371/journal.pone.0171257. eCollection 2017"
COMMENT     Sequence Label: pBAD-LSSmCherry1
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..711
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMTIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGLEASTERMYPEDGALK
                     GESKERLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERS
                     EGRHSTGGMDELYK"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atggtgagca agggcgagga ggataacatg accatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa ttgggagcgc
      301 gtgatgaact ttgaggacgg cggtgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctctga cggccccgta
      421 atgcagtgtc gtaccatggg cttggaggcc agtactgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagagtaa ggagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 gacatcaagt tggacatcct ttcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagta a
//
