LOCUS       sequence-17440-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Chin L, Tam A, Pomerantz J, Wong M, Holash J, Bardeesy N, Shen Q, 
            O'Hagan R, Pantginis J, Zhou H, Horner JW, Cordon-Cardo C, 
            Yancopoulos GD, DePinho RA
  TITLE     Essential role for oncogenic Ras in tumour maintenance.
  JOURNAL   Nature. 1999 Jul 29. 400(6743):468-72.
  PUBMED    10440378
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1999 Jul 29. 400(6743):468-72."
COMMENT     Sequence Label: tet operator
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    58..76
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    100..118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    142..160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    184..202
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    226..244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        277..315
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     312..336
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(443..459)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(444..460)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(488..506)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(527..543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(527..543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(540..562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    551..567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(575..581,582..599,600..605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    620..641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ccctcgagtt tacactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
       61 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
      121 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      181 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      241 gagaaaagtg aaagtcgagc tcggtacccg ggtcgaggta ggcgtgtacg gtgggaggcc
      301 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgccn tccacgctgt
      361 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga attgggctgc
      421 aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc ggtaccagct
      481 tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc
      541 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      601 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      661 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc ca
//
