LOCUS       sequence-174400-        8345 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing LexAop2-myr4x::CLIPf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 8345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: LexAop2-myr::4xCLIPf
FEATURES             Location/Qualifiers
     source          1..8345
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(45..63)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        130..234
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(235..303,304..1095)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(453..472)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1266..1854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1755..1774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1941..2010
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2235..2253)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2705..2776,3178..3451,3526..4180,4242..4557,4761..4892,
                     4963..5577)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        6989..7227
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7174..7195
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          7242..7308
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
ORIGIN
        1 agctggcttg gatagcgatt cgagttaacc ttgaagacga aagggcctcg tgatacgcct
       61 atttttatag gttaatgtca tgataataat ggtttcttag actcaggtgg cacttttcgg
      121 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      181 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      241 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      301 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      361 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      421 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      481 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
      541 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      601 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
      661 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
      721 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
      781 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
      841 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
      901 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      961 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1021 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1081 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1141 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1201 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1261 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1321 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1381 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1441 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1501 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1561 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1621 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1681 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1741 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1801 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1861 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttaccg
     1921 tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc
     1981 tccccgggcg cgtactccac ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg
     2041 cggtagttga tcccggcgaa cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg
     2101 ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg
     2161 ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt cgacaagccg aacatatggg
     2221 cgcgcctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
     2281 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
     2341 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
     2401 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
     2461 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
     2521 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     2581 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     2641 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     2701 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     2761 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     2821 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     2881 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     2941 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     3001 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     3061 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     3121 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     3181 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     3241 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     3301 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     3361 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     3421 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     3481 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     3541 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     3601 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     3661 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     3721 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     3781 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     3841 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     3901 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     3961 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     4021 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     4081 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     4141 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     4201 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     4261 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     4321 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     4381 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     4441 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     4501 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     4561 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     4621 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     4681 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     4741 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     4801 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     4861 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     4921 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     4981 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     5041 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     5101 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     5161 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     5221 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     5281 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     5341 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     5401 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     5461 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     5521 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     5581 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     5641 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     5701 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     5761 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     5821 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     5881 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     5941 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     6001 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     6061 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     6121 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     6181 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     6241 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     6301 agaatacatt taatttagaa aatgcttgga tttcactgga actagggcgc gcctccggaa
     6361 cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa
     6421 gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
     6481 ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
     6541 cctcaacgac aggagcacga tcatgcgcac ccgtggccag ggccgcaagc ttgcatgcct
     6601 gcaggttact gtacatccat acagtaagta ctgtacatcc atacagtaag tactgtacat
     6661 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga
     6721 gactctagcc ctagggcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca
     6781 tccatacagt aagtactgta catccataca gtaagcggag actctagcgc tagcgcatgc
     6841 ctgcaggtta ctgtacatcc atacagtaag tactgtacat ccatacagta agtactgtac
     6901 atccatacag taagtactgt acatccatac agtaagtact gtacatccat acagtaagcg
     6961 gagactctag cactagtgac gtcgagcgcc ggagtataaa tagaggcgct tcgtctacgg
     7021 agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat
     7081 aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca
     7141 attaaaagta accagcaacc aagtaaatca actgcaacta ctgaaatctg ccaagaagta
     7201 attattgaat acaagaagag aactctgaat agatctaaaa ggtaggttca accactgatg
     7261 cctaggcaca ccgaaacgac taaccctaat tcttatcctt tacttcaggc aattacaaat
     7321 tatataacaa aatgggcaac aaatgctgca gcaagcgaca ggatcaggaa ctggcactgg
     7381 cctatcccac tgggggctac aagaaatccg actacacctt tggccagacg cacatcaaca
     7441 gcagcggcgg cggcaacatg ggcggcgttc ttggccagaa gcataacaac ggtggctcgc
     7501 tggactcgcg ctacacgccc gatcccaatc atcggggtcc gttgaaaatc ggcggaaagg
     7561 gcggcgttga catcatcaga ccacgcgtga tggataagga ttgcgagatg aagcgtacaa
     7621 cattggattc cccgttggga aagttggagt tgtccggatg cgagcaggga ttgcaccgca
     7681 ttatcttctt gggaaagggc acctccgctg ctgatgctgt cgaggtcccc gctccggctg
     7741 ctgtcttggg aggccccgag cccttgatcc aggctacggc ttggttgaac gcttacttcc
     7801 accagcccga ggctattgag gagttccccg tccccgcttt gcaccacccc gtcttccagc
     7861 aggagtcctt cacgcgtcag gtcttgtgga agttgctgaa ggtcgtgaag ttcggcgagg
     7921 tgatctccga gtcccacttg gctgccttgg tgggaaaccc ggctgccacg gccgctgtca
     7981 acacggcttt ggatggcaac cccgtcccga tcttgatccc gtgccaccgt gtggtccagg
     8041 gagattccga tgtcggaccc tacctgggag gcctggctgt caaggagtgg ttgttggctc
     8101 acgagggaca ccgcttggga aagcccggct tgggaagcgt aagcggtagt acaatggata
     8161 aggattgcga gatgaagcgt acaacattgg attccccgtt gggaaagttg gagttgtccg
     8221 gatgcgagca gggattgcac cgcattatct tcttgggaaa gggcacctcc gctgctgatg
     8281 ctgtcgaggt ccccgctccg gctgctgtct tgggaggccc cgagcccttg atccaggcta
     8341 cggct
//
