LOCUS       sequence-174403-        9735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing 
            LexAop2->Bxb1.STOP>myr::4xSNAPf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 9735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: LexAop2->Bxb1.STOP>myr::4xSNAPf
FEATURES             Location/Qualifiers
     source          1..9735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(882..900)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        967..1071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1072..1140,1141..1932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1290..1309)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2103..2691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2592..2611
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2778..2847
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(3072..3090)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3542..3613,4015..4288,4363..5017,5079..5394,5598..5729,
                     5800..6414)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        7826..8064
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     8011..8032
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          8079..8145
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     protein_bind    8426..8473
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             8478..8567
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(8591..8607)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8592..8608)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     intron          8699..8764
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8894..8914
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9186..9320
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9223..9242)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9277..9296
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9322..9359
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 ctgctacagc cgccgttaaa accgccctga gcggaaatcc cgtgcccatt ctgatcccct
       61 gccaccgggt ggtgcagggc gacctggacg tgggaggcta cgagggcggg ctcgccgtga
      121 aagagtggct gctggcccac gagggccaca gactgggcaa gcctgggctg ggttagtgac
      181 tcgagactag ttaatctaga atgaatcgtt tttaaaataa caaatcaatt gttttataat
      241 attcgtacga ttctttgatt atgtaataaa atgtgatcat taggaagatt acgaaaaata
      301 taaaaaatat gagttctgtg tgtataacaa atgctgtaaa cgccacaatt gtgtttgttg
      361 caaataaacc catgattatt tgattaaaat tgttgttttc tttgttcata gacaatagtg
      421 tgttttgcct aaacgtgtac tgcataaact ccatgcgagt gtatagcgag ctagtggcta
      481 acgcttgccc caccaaagta gattcgtcaa aatcctcaat ttcatcaccc tcctccaagt
      541 ttaacatttg gccgtcggaa ttaacttcta aagatgccac ataatctaat aaatgaaata
      601 gagattcaaa cgtggcgtca tcgtccgttt cgaccatttc cgaaaagaac tcgggcataa
      661 actctatgat ttctctggac gtggtgttgt cgaaactctc aaagtacgca gtcaggaacg
      721 tgcgcgacat gtcgtcggga aactcgcgcg gaaacatgtt gttgtaaccg aacgggtccc
      781 atagcgccaa aaccaaatct gccagcgtca atagaatgag cacgatgccg acaatggagc
      841 tggcttggat agcgattcga gttaaccttg aagacgaaag ggcctcgtga tacgcctatt
      901 tttataggtt aatgtcatga taataatggt ttcttagact caggtggcac ttttcgggga
      961 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1021 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1081 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1141 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1201 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1261 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1321 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1381 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1441 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1501 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     1561 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     1621 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1681 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1741 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     1801 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1861 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1921 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1981 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2041 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2101 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2161 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2221 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2281 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2341 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2401 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2461 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2521 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2581 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2641 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2701 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttaccgtcg
     2761 acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc
     2821 ccgggcgcgt actccacctc acccatctgg tccatcatga tgaacgggtc gaggtggcgg
     2881 tagttgatcc cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa accgctgggc
     2941 gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc
     3001 agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga caagccgaac atatgggcgc
     3061 gcctagtatg tatgtaagtt aataaaaccc atttttgcgg aaagtagata aaaaaaacat
     3121 tttttttttt tactgcactg gatatcattg aacttatctg atcagtttta aatttacttc
     3181 gatccaaggg tatttgatgt accaggttct ttcgattacc tctcactcaa aatgacattc
     3241 cactcaaagt cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc cgtctctttc
     3301 gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg ttacgtagcg tcaatgtccg
     3361 ccttcagttg cattttgtca gcggtttcgt gacgaagctc caagcggttt acgccatcaa
     3421 ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt atttttgttt gttttttgag
     3481 tgattggggt ggtgattggt tttgggtggg taagcagggg aaagtgtgaa aaatcccggc
     3541 aatgggccaa gaggatcagg agctattaat tcgcggaggc agcaaacacc catctgccga
     3601 gcatctgaac aatgtgagta gtacatgtgc atacatctta agttcacttg atctatagga
     3661 actgcgattg caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa
     3721 accgcaattg cacaaacaaa tagtgacacg aaacagatta ttctggtagc tgttctcgct
     3781 atataagaca atttttgaga tcatatcatg atcaagacat ctaaaggcat tcattttcga
     3841 ctatattctt ttttacaaaa aatataacaa ccagatattt taagctgatc ctagatgcac
     3901 aaaaaataaa taaaagtata aacctacttc gtaggatact tcggggtact ttttgttcgg
     3961 ggttagatga gcataacgct tgtagttgat atttgagatc ccctatcatt gcagggtgac
     4021 agcggagcgg cttcgcagag ctgcattaac cagggcttcg ggcaggccaa aaactacggc
     4081 acgctccggc cacccagtcc gccggaggac tccggttcag ggagcggcca actagccgag
     4141 aacctcacct atgcctggca caatatggac atctttgggg cggtcaatca gccgggctcc
     4201 ggatggcggc agctggtcaa ccggacacgc ggactattct gcaacgagcg acacataccg
     4261 gcgcccagga aacatttgct caagaacggt gagtttctat tcgcagtcgg ctgatctgtg
     4321 tgaaatctta ataaagggtc caattaccaa tttgaaactc agtttgcggc gtggcctatc
     4381 cgggcgaact tttggccgtg atgggcagtt ccggtgccgg aaagacgacc ctgctgaatg
     4441 cccttgcctt tcgatcgccg cagggcatcc aagtatcgcc atccgggatg cgactgctca
     4501 atggccaacc tgtggacgcc aaggagatgc aggccaggtg cgcctatgtc cagcaggatg
     4561 acctctttat cggctcccta acggccaggg aacacctgat tttccaagcc atggtgcgga
     4621 tgccacgaca tctgacctat cggcagcgag tggcccgcgt ggatcaggtg atccaggagc
     4681 tttcgctcag caaatgtcag cacacgatca tcggtgtgcc cggcagggtg aaaggtctgt
     4741 ccggcggaga aaggaagcgt ctggcattcg cctccgaggc tctaaccgat ccgccgcttc
     4801 tgatctgcga tgagcccacc tccggactgg actcctttac cgcccacagc gtcgtccagg
     4861 tgctgaagaa gctgtcgcag aagggcaaga ccgtcatcct gaccattcat cagccgtctt
     4921 ccgagctgtt tgagctcttt gacaagatcc ttctgatggc cgagggcagg gtagctttct
     4981 tgggcactcc cagcgaagcc gtcgacttct tttcctagtg agttcgatgt gtttattaag
     5041 ggtatctagt attacataac atctcaactc ctatccagcg tgggtgccca gtgtcctacc
     5101 aactacaatc cggcggactt ttacgtacag gtgttggccg ttgtgcccgg acgggagatc
     5161 gagtcccgtg atcggatcgc caagatatgc gacaattttg ccattagcaa agtagcccgg
     5221 gatatggagc agttgttggc caccaaaaat ctggagaagc cactggagca gccggagaat
     5281 gggtacacct acaaggccac ctggttcatg cagttccggg cggtcctgtg gcgatcctgg
     5341 ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac ttattcagac aacggtgagt
     5401 ggttccagtg gaaacaaatg atataacgct tacaattctt ggaaacaaat tcgctagatt
     5461 ttagatagaa ttgcctgatt ccacaccctt cttagttttt ttcaatgaga tgtatagttt
     5521 atagttttgc agaagataaa taaatttcat ttaactcgcg aatattaatg agatgcgagt
     5581 aacattttaa tttgcagatg gttgccatct tgattggcct catctttttg ggccaacaac
     5641 tcacgcaagt gggtgtgatg aatatcaacg gagccatctt cctcttcctg accaacatga
     5701 cctttcaaaa cgtctttgcc acgataaatg taagtcatgt ttagaataca tttgcatttc
     5761 aataatttac taactttcta atgaatcgat tcgatttagg tgttcacctc agagctgcca
     5821 gtttttatga gggaggcccg aagtcgactt tatcgctgtg acacatactt tctgggcaaa
     5881 acgattgccg aattgccgct ttttctcaca gtgccactgg tcttcacggc gattgcctat
     5941 ccgatgatcg gactgcgggc cggagtgctg cacttcttca actgcctggc gctggtcact
     6001 ctggtggcca atgtgtcaac gtccttcgga tatctaatat cctgcgccag ctcctcgacc
     6061 tcgatggcgc tgtctgtggg tccgccggtt atcataccat tcctgctctt tggcggcttc
     6121 ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc
     6181 cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt
     6241 agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt
     6301 aacttctccg ccgccgatct gccgctggac tacgtgggtc tggccattct catcgtgagc
     6361 ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca
     6421 tatatccgaa ataactgctt gttttttttt tttaccatta ttaccatcgt gtttactgtt
     6481 tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca agatatgacc
     6541 tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg atttgtcttc
     6601 taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat cgctacataa
     6661 aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc ttcataattc
     6721 acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa
     6781 cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc gcgattcggt
     6841 tcgctcaaat ggttccgagt ggttcatttc gtctcaatag aaattagtaa taaatatttg
     6901 tatgtacaat ttatttgctc caatatattt gtatatattt ccctcacagc tatatttatt
     6961 ctaatttaat attatgactt tttaaggtaa ttttttgtga cctgttcgga gtgattagcg
     7021 ttacaatttg aactgaaagt gacatccagt gtttgttcct tgtgtagatg catctcaaaa
     7081 aaatggtggg cataatagtg ttgtttatat atatcaaaaa taacaactat aataataaga
     7141 atacatttaa tttagaaaat gcttggattt cactggaact agggcgcgcc tccggaacat
     7201 aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac
     7261 cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc
     7321 gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct
     7381 caacgacagg agcacgatca tgcgcacccg tggccagggc cgcaagcttg catgcctgca
     7441 ggttactgta catccataca gtaagtactg tacatccata cagtaagtac tgtacatcca
     7501 tacagtaagt actgtacatc catacagtaa gtactgtaca tccatacagt aagcggagac
     7561 tctagcccta gggcatgcct gcaggttact gtacatccat acagtaagta ctgtacatcc
     7621 atacagtaag tactgtacat ccatacagta agcggagact ctagcgctag cgcatgcctg
     7681 caggttactg tacatccata cagtaagtac tgtacatcca tacagtaagt actgtacatc
     7741 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagcggag
     7801 actctagcac tagtgacgtc gagcgccgga gtataaatag aggcgcttcg tctacggagc
     7861 gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa
     7921 caagcgcagc tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt
     7981 aaaagtaacc agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt
     8041 attgaataca agaagagaac tctgaataga tctaaaaggt aggttcaacc actgatgcct
     8101 aggcacaccg aaacgactaa ccctaattct tatcctttac ttcaggcaat tacaaattat
     8161 ataacaaaat gggcaacaaa tgctgcagca agcgacagga tcaggaactg gcactggcct
     8221 atcccactgg gggctacaag aaatccgact acacctttgg ccagacgcac atcaacagca
     8281 gcggcggcgg caacatgggc ggcgttcttg gccagaagca taacaacggt ggctcgctgg
     8341 actcgcgcta cacgcccgat cccaatcatc ggggtccgtt gaaaatcggc ggaaagggcg
     8401 gcgttgacat catcagacca cgcgtggttt gtctggtcaa ccaccgcggt ctcagtggtg
     8461 tacggtacaa acccagttac ccatacgatg ttcctgacta tgcgggctat ccctatgacg
     8521 tcccggacta tgcaggatcc tatccatatg acgttccaga ttacgcttaa gaattcgata
     8581 tcaagcttat cgataccgtc gacctcgagg gtacctctag aggatctttg tgaaggaacc
     8641 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     8701 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     8761 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     8821 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     8881 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     8941 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     9001 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatatttgat
     9061 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     9121 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     9181 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9241 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9301 tatcttatca tgtctggatc cggcttgtcg acgacggcgg tctccgtcgt caggatcatc
     9361 ttatggacaa agactgcgaa atgaagcgca ccaccctgga tagccctctg ggcaagctgg
     9421 aactgtctgg gtgcgaacag ggcctgcacc gtatcatctt cctgggcaaa ggaacatctg
     9481 ccgccgacgc cgtggaagtg cctgccccag ccgccgtgct gggcggacca gagccactga
     9541 tgcaggccac cgcctggctc aacgcctact ttcaccagcc tgaggccatc gaggagttcc
     9601 ctgtgccagc cctgcaccac ccagtgttcc agcaggagag ctttacccgc caggtgctgt
     9661 ggaaactgct gaaagtggtg aagttcggag aggtcatcag ctacagccac ctcgctgccc
     9721 tcgctggcaa tccag
//
