LOCUS       sequence-174408-        9401 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing UAS-Syt::3xHalo7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: UAS-Syt::3xHalo7
FEATURES             Location/Qualifiers
     source          1..9401
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(724..742)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        809..913
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(914..982,983..1774)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1132..1151)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1945..2533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2434..2453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2620..2689
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2914..2932)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3384..3455,3857..4130,4205..4859,4921..5236,5440..5571,
                     5642..6256)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    7286..7380
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    7414..7508
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        7539..7777
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7724..7745
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          7792..7858
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
ORIGIN
        1 agatctacta gttaatctag aatgaatcgt ttttaaaata acaaatcaat tgttttataa
       61 tattcgtacg attctttgat tatgtaataa aatgtgatca ttaggaagat tacgaaaaat
      121 ataaaaaata tgagttctgt gtgtataaca aatgctgtaa acgccacaat tgtgtttgtt
      181 gcaaataaac ccatgattat ttgattaaaa ttgttgtttt ctttgttcat agacaatagt
      241 gtgttttgcc taaacgtgta ctgcataaac tccatgcgag tgtatagcga gctagtggct
      301 aacgcttgcc ccaccaaagt agattcgtca aaatcctcaa tttcatcacc ctcctccaag
      361 tttaacattt ggccgtcgga attaacttct aaagatgcca cataatctaa taaatgaaat
      421 agagattcaa acgtggcgtc atcgtccgtt tcgaccattt ccgaaaagaa ctcgggcata
      481 aactctatga tttctctgga cgtggtgttg tcgaaactct caaagtacgc agtcaggaac
      541 gtgcgcgaca tgtcgtcggg aaactcgcgc ggaaacatgt tgttgtaacc gaacgggtcc
      601 catagcgcca aaaccaaatc tgccagcgtc aatagaatga gcacgatgcc gacaatggag
      661 ctggcttgga tagcgattcg agttaacggc cggccgttta aacgaattct tgaagacgaa
      721 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      781 ctcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      841 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      901 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      961 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1021 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1081 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1141 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     1201 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1261 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1321 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1381 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1441 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1501 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1561 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1621 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     1681 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1741 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1801 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1861 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1921 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1981 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2041 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2101 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2161 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2221 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2281 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2341 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2401 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2461 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2521 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2581 ttttgctcac atgttaccgt cgacgatgta ggtcacggtc tcgaagccgc ggtgcgggtg
     2641 ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct ggtccatcat
     2701 gatgaacggg tcgaggtggc ggtagttgat cccggcgaac gcgcggcgca ccgggaagcc
     2761 ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc acgggacgtg cgacggcgtc
     2821 ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg acggtcacgg cgggcatgtc
     2881 gacaagccga acatatgggc gcgcctagta tgtatgtaag ttaataaaac ccatttttgc
     2941 ggaaagtaga taaaaaaaac attttttttt tttactgcac tggatatcat tgaacttatc
     3001 tgatcagttt taaatttact tcgatccaag ggtatttgat gtaccaggtt ctttcgatta
     3061 cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct tctctgtcca
     3121 cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag
     3181 cgttacgtag cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc gtgacgaagc
     3241 tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc ctctcgcttc
     3301 ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg ggtaagcagg
     3361 ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta attcgcggag
     3421 gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt gcatacatct
     3481 taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa
     3541 ctgcgaccca caaaaatccc aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat
     3601 tattctggta gctgttctcg ctatataaga caatttttga gatcatatca tgatcaagac
     3661 atctaaaggc attcattttc gactatattc ttttttacaa aaaatataac aaccagatat
     3721 tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact tcgtaggata
     3781 cttcggggta ctttttgttc ggggttagat gagcataacg cttgtagttg atatttgaga
     3841 tcccctatca ttgcagggtg acagcggagc ggcttcgcag agctgcatta accagggctt
     3901 cgggcaggcc aaaaactacg gcacgctccg gccacccagt ccgccggagg actccggttc
     3961 agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg acatctttgg
     4021 ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac gcggactatt
     4081 ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg gtgagtttct
     4141 attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc aatttgaaac
     4201 tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag ttccggtgcc
     4261 ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg
     4321 ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat gcaggccagg
     4381 tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag ggaacacctg
     4441 attttccaag ccatggtgcg gatgccacga catctgacct atcggcagcg agtggcccgc
     4501 gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat catcggtgtg
     4561 cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt cgcctccgag
     4621 gctctaaccg atccgccgct tctgatctgc gatgagccca cctccggact ggactccttt
     4681 accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc
     4741 ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat ccttctgatg
     4801 gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt cttttcctag
     4861 tgagttcgat gtgtttatta agggtatcta gtattacata acatctcaac tcctatccag
     4921 cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac aggtgttggc
     4981 cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat gcgacaattt
     5041 tgccattagc aaagtagccc gggatatgga gcagttgttg gccaccaaaa atctggagaa
     5101 gccactggag cagccggaga atgggtacac ctacaaggcc acctggttca tgcagttccg
     5161 ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg
     5221 acttattcag acaacggtga gtggttccag tggaaacaaa tgatataacg cttacaattc
     5281 ttggaaacaa attcgctaga ttttagatag aattgcctga ttccacaccc ttcttagttt
     5341 ttttcaatga gatgtatagt ttatagtttt gcagaagata aataaatttc atttaactcg
     5401 cgaatattaa tgagatgcga gtaacatttt aatttgcaga tggttgccat cttgattggc
     5461 ctcatctttt tgggccaaca actcacgcaa gtgggtgtga tgaatatcaa cggagccatc
     5521 ttcctcttcc tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtaagtcat
     5581 gtttagaata catttgcatt tcaataattt actaactttc taatgaatcg attcgattta
     5641 ggtgttcacc tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg
     5701 tgacacatac tttctgggca aaacgattgc cgaattgccg ctttttctca cagtgccact
     5761 ggtcttcacg gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt
     5821 caactgcctg gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat
     5881 atcctgcgcc agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc
     5941 attcctgctc tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg
     6001 gttgtcgtac ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc
     6061 ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg
     6121 caaggtcatc ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg
     6181 tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc
     6241 ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt tttttaccat
     6301 tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg
     6361 ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca
     6421 agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa
     6481 aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa
     6541 tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct
     6601 gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt
     6661 cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt tcgtctcaat
     6721 agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat ttgtatatat
     6781 ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt aattttttgt
     6841 gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc
     6901 cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag tgttgtttat atatatcaaa
     6961 aataacaact ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa
     7021 ctagggcgcg cctccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     7081 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     7141 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     7201 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
     7261 gccgcaagct tgcatgcctg caggtcggag tactgtcctc cgagcggagt actgtcctcc
     7321 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
     7381 cggagactct agccctaggg catgcctgca ggtcggagta ctgtcctccg agcggagtac
     7441 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
     7501 cctccgagcg gagactctag cgctagcgac gtcgagcgcc ggagtataaa tagaggcgct
     7561 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
     7621 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta
     7681 aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta ctgaaatctg
     7741 ccaagaagta attattgaat acaagaagag aactctgaat agatctaaaa ggtaggttca
     7801 accactgatg cctaggcaca ccgaaacgac taaccctaat tcttatcctt tacttcaggc
     7861 ggccgcaact cctaaaaaac cgccaccatg ccgccaaatg caaaatcgga aacggacgcc
     7921 aaaccggaag cggaaccagc gccagcgtcc gagccggcag cggaactgga gtccgtcgac
     7981 cagaagctgg aagaaacaca tcactccaaa ttccgtgaag tggacaggca ggagcaggag
     8041 gtgctcgccg agaaggcggc ggaagcggcc agtcaaagaa tcgcacaggt ggaatcaaca
     8101 acacggagtg ctaccacaga ggctcaggaa tccaccacca ccgcagtgcc ggtgatcaaa
     8161 aagatcgagc atgtcggcga agtggtcacc gaggtgatcg cggagcgcac ggggctgccc
     8221 acatggggcg tggtggccat catcatactc gtgttcctcg tcgtctttgg tataatcttc
     8281 ttctgtgtgc ggagattcct gaagaagcga agaaccaaag atggaaaggg taagaagggt
     8341 gtcgacatga agtcggtaca gttgttggga tcggcgtaca aggagaaagt tcagcctgat
     8401 atggaggaac tcaccgaaaa tgccgaggag ggtgacgagg aggacaagca gagcgagcag
     8461 aagctggggc gcctcaactt caagctggaa tacgatttca actccaacag tttggccgtg
     8521 acggtgatcc aagccgagga gcttcccgcc ctggatatgg gcggtacctc ggatccctat
     8581 gtcaaggtgt acttgctgcc cgacaagaag aagaagttcg agaccaaggt gcaccgcaag
     8641 acactgagtc cggtcttcaa cgagacgttc acctttaagg gtttgcccta cgccgatgcc
     8701 atgaacaaga cgctcgtgtt tgccattttc gacttcgatc gcttctcgaa gcacgaccag
     8761 atcggcgagg tgaaggtgcc gctgtgcacc atcgatctgg cgcagacaat cgaggagtgg
     8821 cgcgacctgg tcagcgttga gggagagggc ggacaggaaa agctgggaga tatctgcttc
     8881 tcgctgcgct acgtgccgac cgccggaaag ctaaccgtcg tcatcctgga ggccaagaac
     8941 ttgaagaaga tggacgtggg cggactgtct gatccatatg tgaaaattgc aatcatgcaa
     9001 aatggcaaac gtttgaaaaa gaggaagaca agtgtcaaaa aatgcaccct caacccttac
     9061 tataatgagt cgttctcatt tgaagtacca tttgaacaaa tgcaaaaaat ctgtctcgct
     9121 gtgaccgtcg tggactacga tcgtattggc acctccgaac ccatcggccg ctgcatactt
     9181 ggctgcatgg gcaccggaac cgagctgcgt cactggtcgg acatgttggc ctcgccccgc
     9241 cggcccatcg cccagtggca caccctcaag gatcccgagg agaccgacga gatcctgaag
     9301 aacatgaaga ccggttctag aggatccatg gcagaaatcg gtactggctt tccattcgac
     9361 ccccattatg tggaagtcct gggcgagcgc atgcactacg t
//
