LOCUS       sequence-174438-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an inducible short hairpin targeting human MKP1 sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: tet-pLKO shMKP1.v1 puro
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      18..606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     507..526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     760..777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    894..915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(930..935,936..953,954..960)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    968..984
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     973..995
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     992..1008
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     992..1008
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1027..1047
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1029..1047
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1075..1301
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1302..1482
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1529..1654
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2147..2380
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2565..2609
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2898..2992
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2909..2928
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2946..2964
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2974..2992
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    3093..3210
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3259..3769
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3688..3706
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3778..4350
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4358..4377
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4405..4423
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4434..5054
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4507..4526)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    5089..5662
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5256..5273)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5483..5502
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5682..6281
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5682..5701)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6178..6198
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6409..6642
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6714..6835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6751..6770)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6805..6824
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6875..7010
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7031..7049)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7059..7076)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7059..7075)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7068..7090)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7217..7672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7304..7323)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7514..7535
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7698..7802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7803..7871,7872..8663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8021..8040)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
       61 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      121 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      181 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      241 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      301 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      361 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      421 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      481 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      541 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      601 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      661 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      721 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      781 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      841 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      901 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      961 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     1021 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     1081 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     1141 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     1201 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     1261 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     1321 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     1381 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     1441 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     1501 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     1561 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     1621 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     1681 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     1741 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     1801 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1861 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1921 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     1981 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     2041 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     2101 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     2161 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     2221 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2281 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2341 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2401 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2461 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2521 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2581 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2641 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2701 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2761 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2821 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact
     2881 agcctcgagc ggccgcaata tttgcatgtc gctatgtgtt ctgggaaatc accataaacg
     2941 tgaaatccct atcagtgata gagacttata agttccctat cagtgataga gacaccgggc
     3001 acattcggga ccaatatatc tgcagatata ttggtcccga atgtgctttt tgaattctcg
     3061 acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     3121 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     3181 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     3241 ctttggccgc ggctcgaggg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc
     3301 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc
     3361 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
     3421 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
     3481 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
     3541 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
     3601 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
     3661 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
     3721 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatctgtg
     3781 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga
     3841 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga
     3901 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt
     3961 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt
     4021 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt
     4081 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
     4141 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
     4201 attggtagaa acaactacat cctggtcatc atcctgcctt tctctttatg gttacaatga
     4261 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
     4321 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     4381 tctcatcatt ttggcaaaga attgtaatac gactcactat agggcgaatt gatatgtcta
     4441 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
     4501 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt
     4561 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc
     4621 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg
     4681 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta
     4741 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
     4801 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag
     4861 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
     4921 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag
     4981 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat
     5041 gtgaaagtgg gtccgcgtac agcggctccc gggagttcta gggatctgcc cctctccctc
     5101 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     5161 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     5221 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     5281 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     5341 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     5401 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     5461 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     5521 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     5581 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     5641 cctttgaaaa acacgatgat aaggatccac cggagcttac catgaccgag tacaagccca
     5701 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     5761 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     5821 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5881 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5941 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6001 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6061 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6121 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6181 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6241 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     6301 agcgcccgac cgaaaggagc gcacgacccc atgcatcggt acctttaaga ccaatgactt
     6361 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6421 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     6481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6661 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6721 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6781 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6841 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6901 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6961 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     7021 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     7081 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     7141 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7201 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7261 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7321 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7381 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7441 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7501 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7561 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7621 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     7681 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7741 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7801 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7861 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7921 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7981 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8041 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8101 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8161 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8221 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8281 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8341 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8401 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8461 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8521 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8581 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8641 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8701 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8761 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaa
//
