LOCUS       sequence-174439-       14714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disturb the expression of miR529 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14714)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM529
FEATURES             Location/Qualifiers
     source          1..14714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..677
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             744..1769
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1810..1984
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2062..2086
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2511..3305
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3392..3980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4134..4151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4166..4306
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4232..4251)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4392..4414
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4650..4844
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4910..5983)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6412..7041)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8342..8366
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(8388..8640)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8675..8692)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(8699..10516,10707..10721))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          10517..10706
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(10738..11083)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     11445..11467
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11459..11476
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11460..11476
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11652..11671
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11652..11670
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        12078..12423
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    12582..12758
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(13320..13338)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(13321..13338)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     13977..13996
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(14099..14104,14105..14121,14122..14127))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(14410..14426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14410..14426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14423..14445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14434..14450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14458..14464,14465..14482,14483..14488))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14503..14524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
      301 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
      361 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
      421 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg
      481 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      541 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      601 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      661 tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc tcgagctttc
      721 gcagatccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
      781 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
      841 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
      901 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
      961 cgattccgga agtgcttgac attggggagt ttagcgagag cctgacctat tgcatctccc
     1021 gccgttcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctac
     1081 aaccggtcgc ggaggctatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     1141 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     1201 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     1261 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     1321 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     1381 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     1441 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     1501 atccggagct tgcaggatcg ccacgactcc gggcgtatat gctccgcatt ggtcttgacc
     1561 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     1621 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     1681 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     1741 ccagcactcg tccgagggca aagaaataga gtagatgccg accgggatct gtcgatcgac
     1801 aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc
     1861 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     1921 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca
     1981 gatcccccga attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt
     2041 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     2101 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     2161 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     2221 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     2281 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc
     2341 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     2401 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     2461 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     2521 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     2581 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     2641 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     2701 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     2761 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     2821 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     2881 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     2941 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     3001 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     3061 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     3121 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     3181 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     3241 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     3301 tttagtacct agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3361 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3421 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3481 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     3541 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3601 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3661 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3721 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3781 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4141 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     4201 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4261 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     4321 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     4381 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     4441 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
     4501 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
     4561 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
     4621 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
     4681 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
     4741 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
     4801 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc
     4861 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
     4921 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
     4981 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
     5041 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
     5101 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
     5161 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
     5221 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
     5281 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
     5341 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
     5401 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
     5461 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
     5521 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
     5581 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
     5641 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
     5701 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
     5761 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
     5821 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
     5881 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
     5941 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt
     6001 gggggttcca gtgccattgc agggccggca ggcaacccag ccgcttacgc ctggccaacc
     6061 gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc
     6121 catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact
     6181 ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg
     6241 cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg
     6301 ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc
     6361 cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag
     6421 ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc
     6481 tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat
     6541 acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat
     6601 gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac
     6661 ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct
     6721 tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc
     6781 cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg
     6841 gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc
     6901 ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag
     6961 ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt
     7021 aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc
     7081 atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga
     7141 cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca
     7201 gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac
     7261 acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc
     7321 tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca
     7381 gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg
     7441 gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca
     7501 gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga
     7561 tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct
     7621 cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga
     7681 acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc
     7741 tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc
     7801 tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa
     7861 gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct
     7921 tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc
     7981 tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc
     8041 ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg
     8101 aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag
     8161 aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca
     8221 aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg
     8281 ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta
     8341 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aattcccgat ctagtaacat
     8401 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     8461 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     8521 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
     8581 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
     8641 ggggaaattc gagctggtca cctgtaattc acacgtggtg gtggtggtgg tggctagctt
     8701 gtttgcctcc ctgctgcggt ttttcaccga agttcatgcc agtccagcgt ttttgcagca
     8761 gaaaagccgc cgacttcggt ttgcggtcgc gagtgaagat ccctttcttg ttaccgccaa
     8821 cgcgcaatat gccttgcgag gtcgcaaaat cggcgaaatt ccatacctgt tcaccgacga
     8881 cggcgctgac gcgatcaaag acgcggtgat acatatccag ccatgcacac tgatactctt
     8941 cactccacat gtcggtgtac attgagtgca gcccggctaa cgtatccacg ccgtattcgg
     9001 tgatgataat cggctgatgc agtttctcct gccaggccag aagttctttt tccagtacct
     9061 tctctgccgt ttccaaatcg ccgctttgga cataccatcc gtaataacgg ttcaggcaca
     9121 gcacatcaaa gagatcgctg atggtatcgg tgtgagcgtc gcagaacatt acattgacgc
     9181 aggtgatcgg acgcgtcggg tcgagtttac gcgttgcttc cgccagtggc gcgaaatatt
     9241 cccgtgcacc ttgcggacgg gtatccggtt cgttggcaat actccacatc accacgcttg
     9301 ggtggttttt gtcacgcgct atcagctctt taatcgcctg taagtgcgct tgctgagttt
     9361 ccccgttgac tgcctcttcg ctgtacagtt ctttcggctt gttgcccgct tcgaaaccaa
     9421 tgcctaaaga gaggttaaag ccgacagcag cagtttcatc aatcaccacg atgccatgtt
     9481 catctgccca gtcgagcatc tcttcagcgt aagggtaatg cgaggtacgg taggagttgg
     9541 ccccaatcca gtccattaat gcgtggtcgt gcaccatcag cacgttatcg aatcctttgc
     9601 cacgcaagtc cgcatcttca tgacgaccaa agccagtaaa gtagaacggt ttgtggttaa
     9661 tcaggaactg ttcgcccttc actgccactg accggatgcc gacgcgaagc gggtagatat
     9721 cacactctgt ctggcttttg gctgtgacgc acagttcata gagataacct tcacccggtt
     9781 gccagaggtg cggattcacc acttgcaaag tcccgctagt gccttgtcca gttgcaacca
     9841 cctgttgatc cgcatcacgc agttcaacgc tgacatcacc attggccacc acctgccagt
     9901 caacagacgc gtggttacag tcttgcgcga catgcgtcac cacggtgata tcgtccaccc
     9961 aggtgttcgg cgtggtgtag agcattacgc tgcgatggat tccggcatag ttaaagaaat
    10021 catggaagta agactgcttt ttcttgccgt tttcgtcggt aatcaccatt cccggcggga
    10081 tagtctgcca gttcagttcg ttgttcacac aaacggtgat acgtacactt ttcccggcaa
    10141 taacatacgg cgtgacatcg gcttcaaatg gcgtatagcc gccctgatgc tccatcactt
    10201 cctgattatt gacccacact ttgccgtaat gagtgaccgc atcgaaacgc agcacgatac
    10261 gctggcctgc ccaacctttc ggtataaaga cttcgcgctg ataccagacg ttgcccgcat
    10321 aattacgaat atctgcatcg gcgaactgat cgttaaaact gcctggcaca gcaattgccc
    10381 ggctttcttg taacgcgctt tcccaccaac gctgatcaat tccacagttt tcgcgatcca
    10441 gactgaatgc ccacaggccg tcgagttttt tgatttcacg ggttggggtt tctacaggac
    10501 ggacgagtcg tcggttctgt aactatcatc atcatcatag acacacgaaa taaagtaatc
    10561 agattatcag ttaaagctat gtaatattta caccataacc aatcaattaa aaaatagatc
    10621 agtttaaaga aagatcaaag ctcaaaaaaa taaaaagaga aaagggtcct aaccaagaaa
    10681 atgaaggaga aaaactagaa atttaccctc agatctacca tggtcaagag tcccccgtgt
    10741 tctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
    10801 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
    10861 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
    10921 actgtcggca gaggcatctt caacgatggc ctttccttta tcgcaatgat ggcatttgta
    10981 ggagccacct tccttttcca ctatcttcac aataaagtga cagatagctg ggcaatggaa
    11041 tccgaggagg tttccggata ttaccctttg ttgaaaagtc tcaattgccc tttggtcttc
    11101 tgagactgta tctttgatat ttttggagta gacaagtgtg tcgtgctcca ccatgttgac
    11161 gaagattttc ttcttgtcat tgagtcgtaa gagactctgt atgaactgtt cgccagtctt
    11221 tacggcgagt tctgttaggt cctctatttg aatctttgac tccatgaagc taaactgaag
    11281 gcgggaaacg acaatctgat ccaagctcaa gctgctctag cattcgccat tcaggctgcg
    11341 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    11401 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    11461 taaaacgacg gccagtgcca agcttgcatg cctgcagtta attaagtttg caagcagcag
    11521 attacgcgca gaaaaaaagg atctcaagga gatcctttgg atcacaaaaa aaaagcccgc
    11581 tcattaggcg ggttaaattc ccgggtactg cagccgattc attaatgcag gttaacctgg
    11641 cttatcgaaa ttaatacgac tcactatagg gagaccggca gatctgatcc ctactccaaa
    11701 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
    11761 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
    11821 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
    11881 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
    11941 ggaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
    12001 gacgtaaggg atgacgcaca atcccacccc tactccaaaa atgtcaaaga tactgtctca
    12061 gaagaccaga gggctattga gacttttcaa caaagggtaa tttcgggaaa cctcctcgga
    12121 ttccattgcc cagctatctg tcacctcatc gaaaggacag tagaaaagga aggtggctcc
    12181 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatgcctc tgccgacagt
    12241 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
    12301 acgtcttcga agcaagtgga ttgatgtgac atctccactg acataaggga tgacgcacaa
    12361 ccccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
    12421 acagcccaag cttgaagaag gactagaggg tacaggttgt tgttgttatg gtctaattta
    12481 aatatggtct aaagaagaag aataagctgt actcctatct ctcttctgaa ttcggtacgc
    12541 tgaaatcacc agtctctctc tacaaatcta tctctctcta ttttctccat aaataatgtg
    12601 tgagtagttt cccgataagg gaaattaggg ttcttatagg gtttcgctca tgtgttgagc
    12661 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
    12721 aattcctaaa accaaaatcc agtactaaaa tccagatctc ctaaagtccc tatagatctt
    12781 tgtcgtgaat ataaaccaga cacgagacga ctagacctgg agcccagacg ccgttcgaag
    12841 ctagaagtac cgcttaggca ggaggccgtt agggaaaaga tgctaaggca gggttggtta
    12901 cgttgactcc cccgtaggtt tggtttaaat atgatgaagt ggacggaagg aaggaggaag
    12961 acaaggaagg ataaggttgc aggccctgtg caaggtaaga agatggaaat ttgatagagg
    13021 tacgctacta tacttatact atacgctaag ggaatgattg tatttatacc ctataccccc
    13081 taataacccc ttatcaattt aagaaataat ccgcataagc ccccgcttaa aaattggtat
    13141 cagagccatg aataggtcta tgaccaaaac tcaagaggat aaaacctcac caaaatacga
    13201 aagagtcctt aactctaaag ataaaagatc tttcaagatc aaaactagtt ccctcacacc
    13261 ggtgacgggg atcgcatgcg acctctagac catcacattg gagcttcagc tgctcgagtt
    13321 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
    13381 cgttcttacg tcgacgtacc aattacgccc cgccctgtca ctcatcgcag tactgttgta
    13441 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
    13501 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
    13561 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
    13621 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
    13681 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
    13741 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
    13801 cactatccca tattaccagc tcaccgtctt tcattgccat acggaactcc ggatgagcat
    13861 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
    13921 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
    13981 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
    14041 atccagtgat ttctttctcc attttagctt ccttagctcc tgaaaatctc gataactcat
    14101 taaagcttat cgatgataag ctgtcaaaca tgagaattta gcccgcctaa tgagcgggct
    14161 ttttgttgtg atccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    14221 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    14281 cgtttccccc ctggaagctc cctcgtgcgc tctcctgttc tgaccctgcc gcttaccgga
    14341 tacctgtccg cctttcttaa ttaatctagc tagaggatcc ccgggtaccg agctcgaatt
    14401 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    14461 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    14521 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    14581 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
    14641 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
    14701 gaccaaaggg ctat
//
