LOCUS       sequence-174441-       14716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disturb the expression of miR398 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14716)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM398
FEATURES             Location/Qualifiers
     source          1..14716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    191..212
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(227..232,233..250,251..257)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    265..281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     270..292
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     289..305
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     289..305
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     505..524
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        505..523
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        931..1276
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    1437..1613
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(2175..2193)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2176..2193)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2832..2851
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2954..2959,2960..2976,2977..2982))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(3241..3258)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3241..3257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3250..3272)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3634..3979
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3996..4010,4201..6018)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          4011..4200
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             6025..6042
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      6077..6329
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6351..6375
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7676..8305
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8734..9807
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9873..10067
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10303..10325)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10411..10551
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10466..10485
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10566..10583)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10737..11325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11412..12206)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12631..12655
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12733..12907
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12948..13973)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(14040..14716)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
       61 tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt
      121 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      181 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      241 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      301 atgacatgat tacgaattcg agctcggtac ccggggatcc tctagctaga ttaattaagt
      361 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa ggagatcctt tggatcacaa
      421 aaaaaaagcc cgctcattag gcgggttaaa ttcccgggta ctgcagccga ttcattaatg
      481 caggttaacc tggcttatcg aaattaatac gactcactat agggagaccg gcagatctga
      541 tccctactcc aaaaatgcca aagatacagt ctcagaagac caaagggcta ttgagacttt
      601 tcaacaaagg gtaatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
      661 catcgaaagg acagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
      721 aaaggctatc attcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
      781 gaggagcatc gtgggaaaag aagacgctcc aaccacgtct tcaaagcaag tggattgatg
      841 tgacatctcc actgacgtaa gggatgacgc acaatcccac ccctactcca aaaatgtcaa
      901 agatactgtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatttcggg
      961 aaacctcctc ggattccatt gcccagctat ctgtcacctc atcgaaagga cagtagaaaa
     1021 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatgc
     1081 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     1141 agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca ctgacataag
     1201 ggatgacgca caaccccact atccttcgca agacccttcc tctatataag gaagttcatt
     1261 tcatttggag aggacagccc aagcttaagg ggtgacccta tgagaacaca gttgttgttg
     1321 ttatggtcta atttaaatat ggtctaaaga agaagaatca ggggcgaccc tatgagaaca
     1381 cagaattcgg tacgctgaaa tcaccagtct ctctctacaa atctatctct ctctattttc
     1441 tccataaata atgtgtgagt agtttcccga taagggaaat tagggttctt atagggtttc
     1501 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     1561 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atctcctaaa
     1621 gtccctatag atctttgtcg tgaatataaa ccagacacga gacgactaga cctggagccc
     1681 agacgccgtt cgaagctaga agtaccgctt aggcaggagg ccgttaggga aaagatgcta
     1741 aggcagggtt ggttacgttg actcccccgt aggtttggtt taaatatgat gaagtggacg
     1801 gaaggaagga ggaagacaag gaaggataag gttgcaggcc ctgtgcaagg taagaagatg
     1861 gaaatttgat agaggtacgc tactatactt atactatacg ctaagggaat gattgtattt
     1921 ataccctata ccccctaata accccttatc aatttaagaa ataatccgca taagcccccg
     1981 cttaaaaatt ggtatcagag ccatgaatag gtctatgacc aaaactcaag aggataaaac
     2041 ctcaccaaaa tacgaaagag tccttaactc taaagataaa agatctttca agatcaaaac
     2101 tagttccctc acatcggtga cggggatcgc atgcgacctc tagaccatca cattggagct
     2161 tcagctgctc gagttctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     2221 tattaattgt agccgcgttc ttacgtcgac gtaccaatta cgccccgccc tgtcactcat
     2281 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     2341 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     2401 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     2461 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     2521 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     2581 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     2641 tgtaacaagg gtgaacacta tcccatatta ccagctcacc gtctttcatt gccatacgga
     2701 actccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     2761 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     2821 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     2881 tatcaacggt ggtatatcca gtgatttctt tctccatttt agcttcctta gctcctgaaa
     2941 atctcgataa ctcattaaag cttatcgatg ataagctgtc aaacatgaga atttagcccg
     3001 cctaatgagc gggctttttg ttgtgatccg cgttgctggc gtttttccat aggctccgcc
     3061 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3121 tataaagata ccaggcgttt cccccctgga agctccctcg tgcgctctcc tgttccgacc
     3181 ctgccgctta ccggatacct gtccgccttt cttaattaac tgcaggcatg caagcttggc
     3241 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     3301 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     3361 cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca
     3421 gattgtcgtt tcccgccttc agtttagctt catggagtca aagattcaaa tagaggacct
     3481 aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac gactcaatga
     3541 caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact ccaaaaatat
     3601 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc
     3661 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga
     3721 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga
     3781 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     3841 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
     3901 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     3961 atttcatttg gagagaacac gggggactct tgaccatggt agatctgagg gtaaatttct
     4021 agtttttctc cttcattttc ttggttagga cccttttctc tttttatttt tttgagcttt
     4081 gatctttctt taaactgatc tattttttaa ttgattggtt atggtgtaaa tattacatag
     4141 ctttaactga taatctgatt actttatttc gtgtgtctat gatgatgatg atagttacag
     4201 aaccgacgac tcgtccgtcc tgtagaaacc ccaacccgtg aaatcaaaaa actcgacggc
     4261 ctgtgggcat tcagtctgga tcgcgaaaac tgtggaattg atcagcgttg gtgggaaagc
     4321 gcgttacaag aaagccgggc aattgctgtg ccaggcagtt ttaacgatca gttcgccgat
     4381 gcagatattc gtaattatgc gggcaacgtc tggtatcagc gcgaagtctt tataccgaaa
     4441 ggttgggcag gccagcgtat cgtgctgcgt ttcgatgcgg tcactcatta cggcaaagtg
     4501 tgggtcaata atcaggaagt gatggagcat cagggcggct atacgccatt tgaagccgat
     4561 gtcacgccgt atgttattgc cgggaaaagt gtacgtatca ccgtttgtgt gaacaacgaa
     4621 ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg caagaaaaag
     4681 cagtcttact tccatgattt ctttaactat gccggaatcc atcgcagcgt aatgctctac
     4741 accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc gcaagactgt
     4801 aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt tgaactgcgt
     4861 gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt gcaagtggtg
     4921 aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt cacagccaaa
     4981 agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag
     5041 ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa
     5101 gatgcggact tgcgtggcaa aggattcgat aacgtgctga tggtgcacga ccacgcatta
     5161 atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc tgaagagatg
     5221 ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt
     5281 aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa
     5341 gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga gctgatagcg
     5401 cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc ggatacccgt
     5461 ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa actcgacccg
     5521 acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga taccatcagc
     5581 gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca aagcggcgat
     5641 ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga gaaactgcat
     5701 cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca ctcaatgtac
     5761 accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt
     5821 gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt tgcgacctcg
     5881 caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga ccgcaaaccg
     5941 aagtcggcgg cttttctgct gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg
     6001 cagcagggag gcaaacaagc tagccaccac caccaccacc acgtgtgaat tacaggtgac
     6061 cagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     6121 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     6181 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     6241 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     6301 gcgcgcggtg tcatctatgt tactagatcg ggaattaaac tatcagtgtt tgacaggata
     6361 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
     6421 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct
     6481 cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
     6541 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct
     6601 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat
     6661 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg
     6721 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac
     6781 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg
     6841 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta
     6901 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
     6961 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
     7021 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
     7081 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
     7141 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
     7201 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
     7261 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
     7321 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
     7381 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
     7441 tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa
     7501 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
     7561 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
     7621 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
     7681 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
     7741 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
     7801 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
     7861 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
     7921 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
     7981 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
     8041 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
     8101 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
     8161 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
     8221 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
     8281 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
     8341 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
     8401 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
     8461 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
     8521 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
     8581 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
     8641 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa
     8701 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg
     8761 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
     8821 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
     8881 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag
     8941 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
     9001 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
     9061 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
     9121 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
     9181 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
     9241 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
     9301 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
     9361 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
     9421 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
     9481 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
     9541 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
     9601 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
     9661 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
     9721 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
     9781 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
     9841 gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca
     9901 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
     9961 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
    10021 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
    10081 cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc
    10141 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca
    10201 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat
    10261 caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    10321 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    10381 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
    10441 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    10501 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
    10561 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10621 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10681 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10741 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10801 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10861 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10921 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10981 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    11041 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    11101 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    11161 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11221 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11281 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11341 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11401 cattctaggt actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca
    11461 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc
    11521 ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca
    11581 agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg
    11641 ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg
    11701 cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg
    11761 ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa
    11821 tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt
    11881 tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg
    11941 agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt
    12001 ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac
    12061 cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta
    12121 gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat
    12181 tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa
    12241 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
    12301 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
    12361 tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc
    12421 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
    12481 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
    12541 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
    12601 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt
    12661 gacgcttaga caacttaata acacattgcg gacgttttta atgtactgaa ttaacgccga
    12721 attaattcgg gggatctgga ttttagtact ggattttggt tttaggaatt agaaatttta
    12781 ttgatagaag tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca
    12841 tgagcgaaac cctataggaa ccctaattcc cttatctggg aactactcac acattattat
    12901 ggagaaactc gagcttgtcg atcgacagat cccggtcggc atctactcta tttctttgcc
    12961 ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg
    13021 gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat
    13081 cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc
    13141 aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag tcgtggcgat
    13201 cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac
    13261 cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg
    13321 ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa
    13381 tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg
    13441 agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca
    13501 tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc
    13561 ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccata
    13621 gcctccgcga ccggttgtag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg
    13681 acaccctgtg aacggcggga gatgcaatag gtcaggctct cgctaaactc cccaatgtca
    13741 agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg
    13801 tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag
    13861 ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac
    13921 ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatctcat
    13981 tgccccccgg atctgcgaaa gctcgagaga gatagatttg tagagagaga ctggtgattt
    14041 cagcgtgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga
    14101 tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt
    14161 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
    14221 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
    14281 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
    14341 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
    14401 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
    14461 atgttcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
    14521 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
    14581 agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct
    14641 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
    14701 ttgttgaaaa gtctca
//
