LOCUS       sequence-174442-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an inducible short hairpin targeting human MKP5 sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: tet-pLKO shMKP5.v1 puro
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      32..620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     521..540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     774..791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    908..929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(944..949,950..967,968..974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    982..998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     987..1009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1006..1022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1006..1022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1041..1061
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1043..1061
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1089..1315
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1316..1496
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1543..1668
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2161..2394
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2579..2623
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2912..3006
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2923..2942
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2960..2978
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2988..3006
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    3107..3224
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3273..3783
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3702..3720
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3792..4364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4372..4391
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4419..4437
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4448..5068
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4521..4540)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    5103..5676
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5270..5287)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5497..5516
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5696..6295
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5696..5715)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6192..6212
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6423..6656
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6728..6849
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6765..6784)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6819..6838
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6889..7024
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7045..7063)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7073..7090)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7073..7089)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7082..7104)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7231..7686
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7318..7337)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7528..7549
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7712..7816
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7817..7885,7886..8677)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8035..8054)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
       61 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      661 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      721 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      781 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
      841 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      901 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      961 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     1021 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     1081 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     1141 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     1201 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     1261 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     1321 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     1381 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     1441 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     1501 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     1561 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     1621 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     1681 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     1741 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1801 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1861 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1921 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1981 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     2041 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     2101 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     2161 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     2221 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     2281 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     2341 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     2401 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     2461 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     2521 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     2581 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2641 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2701 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2761 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2821 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     2881 tcgatcacga gactagcctc gagcggccgc aatatttgca tgtcgctatg tgttctggga
     2941 aatcaccata aacgtgaaat ccctatcagt gatagagact tataagttcc ctatcagtga
     3001 tagagacacc ggcaaaggca aacgaccaat tatctgcaga taattggtcg tttgcctttg
     3061 tttttgaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     3121 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     3181 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     3241 acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc
     3301 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     3361 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     3421 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     3481 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     3541 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     3601 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     3661 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     3721 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     3781 cagggggatc tgtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
     3841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
     3901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
     3961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     4021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     4081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     4141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     4201 gtggaaatat tcttattggt agaaacaact acatcctggt catcatcctg cctttctctt
     4261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     4321 gcccctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     4381 tggttattgt gctgtctcat cattttggca aagaattgta atacgactca ctatagggcg
     4441 aattgatatg tctagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa
     4501 tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca
     4561 gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga
     4621 gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt
     4681 tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
     4741 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
     4801 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg
     4861 gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag
     4921 ggaaacacct actactgata gtatgccgcc attattacga caagctatcg aattatttga
     4981 tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga
     5041 aaaacaactt aaatgtgaaa gtgggtccgc gtacagcggc tcccgggagt tctagggatc
     5101 tgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5161 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     5221 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5281 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5341 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5401 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5461 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5521 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5581 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5641 ggggacgtgg ttttcctttg aaaaacacga tgataaggat ccaccggagc ttaccatgac
     5701 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     5761 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     5821 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     5881 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     5941 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     6001 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     6061 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     6121 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     6181 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     6241 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     6301 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggtaccttt
     6361 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6421 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6481 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6541 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6601 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     6661 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6721 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6781 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6841 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     6901 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6961 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7021 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     7081 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     7141 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     7201 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     7261 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     7321 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7381 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7441 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7501 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7561 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7621 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     7681 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7741 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7801 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7861 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7921 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7981 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     8041 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8101 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8161 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8221 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     8281 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8341 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8401 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8461 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8521 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8581 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8641 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8701 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8761 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgt
//
